Unipolar (Dendritic) Brush Cells Are Morphologically Complex and Require Tbr2 for Differentiation and Migration
Previous studies demonstrated specific expression of transcription factor Tbr2 in unipolar brush cells (UBCs) of the cerebellum during development and adulthood. To further study UBCs and the role of Tbr2 in their development we examined UBC morphology in transgenic mouse lines (reporter and lineage...
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doaj-2c6db8e45adf44d396ba09b2448b19f22021-01-08T06:32:24ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2021-01-011410.3389/fnins.2020.598548598548Unipolar (Dendritic) Brush Cells Are Morphologically Complex and Require Tbr2 for Differentiation and MigrationAshley McDonough0Gina E. Elsen1Ray M. Daza2Ray M. Daza3Amelia R. Bachleda4Donald Pizzo5Olivia M. DelleTorri6Robert F. Hevner7Robert F. Hevner8Robert F. Hevner9Center for Integrative Brain Research, Seattle Children’s Research Institute, Seattle, WA, United StatesCenter for Integrative Brain Research, Seattle Children’s Research Institute, Seattle, WA, United StatesCenter for Integrative Brain Research, Seattle Children’s Research Institute, Seattle, WA, United StatesDepartment of Pathology, University of California, San Diego, CA, United StatesCenter for Integrative Brain Research, Seattle Children’s Research Institute, Seattle, WA, United StatesDepartment of Pathology, University of California, San Diego, CA, United StatesCalifornia Institute for Regenerative Medicine, California State University San Marcos, San Marcos, CA, United StatesCenter for Integrative Brain Research, Seattle Children’s Research Institute, Seattle, WA, United StatesDepartment of Pathology, University of California, San Diego, CA, United StatesDepartment of Neurological Surgery, University of Washington, Seattle, WA, United StatesPrevious studies demonstrated specific expression of transcription factor Tbr2 in unipolar brush cells (UBCs) of the cerebellum during development and adulthood. To further study UBCs and the role of Tbr2 in their development we examined UBC morphology in transgenic mouse lines (reporter and lineage tracer) and also examined the effects of Tbr2 deficiency in Tbr2 (MGI: Eomes) conditional knock-out (cKO) mice. In Tbr2 reporter and lineage tracer cerebellum, UBCs exhibited more complex morphologies than previously reported including multiple dendrites, bifurcating dendrites, and up to four dendritic brushes. We propose that “dendritic brush cells” (DBCs) may be a more apt nomenclature. In Tbr2 cKO cerebellum, mature UBCs were completely absent. Migration of UBC precursors from rhombic lip to cerebellar cortex and other nuclei was impaired in Tbr2 cKO mice. Our results indicate that UBC migration and differentiation are sensitive to Tbr2 deficiency. To investigate whether UBCs develop similarly in humans as in rodents, we studied Tbr2 expression in mid-gestational human cerebellum. Remarkably, Tbr2+ UBC precursors migrate along the same pathways in humans as in rodent cerebellum and disperse to create the same “fountain-like” appearance characteristic of UBCs exiting the rhombic lip.https://www.frontiersin.org/articles/10.3389/fnins.2020.598548/fullunipolar brush cellsTbr2cerebellumdevelopmentcell migration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ashley McDonough Gina E. Elsen Ray M. Daza Ray M. Daza Amelia R. Bachleda Donald Pizzo Olivia M. DelleTorri Robert F. Hevner Robert F. Hevner Robert F. Hevner |
spellingShingle |
Ashley McDonough Gina E. Elsen Ray M. Daza Ray M. Daza Amelia R. Bachleda Donald Pizzo Olivia M. DelleTorri Robert F. Hevner Robert F. Hevner Robert F. Hevner Unipolar (Dendritic) Brush Cells Are Morphologically Complex and Require Tbr2 for Differentiation and Migration Frontiers in Neuroscience unipolar brush cells Tbr2 cerebellum development cell migration |
author_facet |
Ashley McDonough Gina E. Elsen Ray M. Daza Ray M. Daza Amelia R. Bachleda Donald Pizzo Olivia M. DelleTorri Robert F. Hevner Robert F. Hevner Robert F. Hevner |
author_sort |
Ashley McDonough |
title |
Unipolar (Dendritic) Brush Cells Are Morphologically Complex and Require Tbr2 for Differentiation and Migration |
title_short |
Unipolar (Dendritic) Brush Cells Are Morphologically Complex and Require Tbr2 for Differentiation and Migration |
title_full |
Unipolar (Dendritic) Brush Cells Are Morphologically Complex and Require Tbr2 for Differentiation and Migration |
title_fullStr |
Unipolar (Dendritic) Brush Cells Are Morphologically Complex and Require Tbr2 for Differentiation and Migration |
title_full_unstemmed |
Unipolar (Dendritic) Brush Cells Are Morphologically Complex and Require Tbr2 for Differentiation and Migration |
title_sort |
unipolar (dendritic) brush cells are morphologically complex and require tbr2 for differentiation and migration |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neuroscience |
issn |
1662-453X |
publishDate |
2021-01-01 |
description |
Previous studies demonstrated specific expression of transcription factor Tbr2 in unipolar brush cells (UBCs) of the cerebellum during development and adulthood. To further study UBCs and the role of Tbr2 in their development we examined UBC morphology in transgenic mouse lines (reporter and lineage tracer) and also examined the effects of Tbr2 deficiency in Tbr2 (MGI: Eomes) conditional knock-out (cKO) mice. In Tbr2 reporter and lineage tracer cerebellum, UBCs exhibited more complex morphologies than previously reported including multiple dendrites, bifurcating dendrites, and up to four dendritic brushes. We propose that “dendritic brush cells” (DBCs) may be a more apt nomenclature. In Tbr2 cKO cerebellum, mature UBCs were completely absent. Migration of UBC precursors from rhombic lip to cerebellar cortex and other nuclei was impaired in Tbr2 cKO mice. Our results indicate that UBC migration and differentiation are sensitive to Tbr2 deficiency. To investigate whether UBCs develop similarly in humans as in rodents, we studied Tbr2 expression in mid-gestational human cerebellum. Remarkably, Tbr2+ UBC precursors migrate along the same pathways in humans as in rodent cerebellum and disperse to create the same “fountain-like” appearance characteristic of UBCs exiting the rhombic lip. |
topic |
unipolar brush cells Tbr2 cerebellum development cell migration |
url |
https://www.frontiersin.org/articles/10.3389/fnins.2020.598548/full |
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