Surveillance Study of Hepatitis E Virus (HEV) in Domestic and Wild Ruminants in Northwestern Italy
In industrialized countries, increasing autochthonous infections of hepatitis E virus (HEV) are caused by zoonotic transmission of genotypes (Gts) 3 and 4, mainly through consumption of contaminated raw or undercooked pork meat. Although swine and wild boar are recognized as the main reservoir for G...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-12-01
|
Series: | Animals |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-2615/10/12/2351 |
id |
doaj-2cabe55739a24be7ac2b2eb4d5dae163 |
---|---|
record_format |
Article |
spelling |
doaj-2cabe55739a24be7ac2b2eb4d5dae1632020-12-10T00:04:00ZengMDPI AGAnimals2076-26152020-12-01102351235110.3390/ani10122351Surveillance Study of Hepatitis E Virus (HEV) in Domestic and Wild Ruminants in Northwestern ItalyAndrea Palombieri0Serena Robetto1Federica Di Profio2Vittorio Sarchese3Paola Fruci4Maria Cristina Bona5Giuseppe Ru6Riccardo Orusa7Fulvio Marsilio8Vito Martella9Barbara Di Martino10Faculty of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta (IZS PLV), 11020 Quart, ItalyFaculty of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyFaculty of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyFaculty of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta (IZS PLV), 11020 Quart, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta (IZS PLV), 11020 Quart, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta (IZS PLV), 11020 Quart, ItalyFaculty of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyDepartment of Veterinary Medicine, Università Aldo Moro di Bari, Valenzano, 70121 Bari, ItalyFaculty of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyIn industrialized countries, increasing autochthonous infections of hepatitis E virus (HEV) are caused by zoonotic transmission of genotypes (Gts) 3 and 4, mainly through consumption of contaminated raw or undercooked pork meat. Although swine and wild boar are recognized as the main reservoir for Gt3 and Gt4, accumulating evidence indicates that other animal species, including domestic and wild ruminants, may harbor HEV. Herein, we screened molecularly and serologically serum and fecal samples from two domestic and four wild ruminant species collected in Valle d’Aosta and Piemonte regions (northwestern Italy. HEV antibodies were found in sheep (21.6%), goats (11.4%), red deer (2.6%), roe deer (3.1%), and in Alpine ibex (6.3%). Molecular screening was performed using different primer sets targeting highly conserved regions of hepeviruses and HEV RNA, although at low viral loads, was detected in four fecal specimens (3.0%, 4/134) collected from two HEV seropositive sheep herds. Taken together, the data obtained document the circulation of HEV in the geographical area assessed both in wild and domestic ruminants, but with the highest seroprevalence in sheep and goats. Consistently with results from other studies conducted in southern Italy, circulation of HEV among small domestic ruminants seems to occur more frequently than expected.https://www.mdpi.com/2076-2615/10/12/2351hepatitis E virus (HEV)domestic and wild ruminantsHEV antibodiesviral RNA. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andrea Palombieri Serena Robetto Federica Di Profio Vittorio Sarchese Paola Fruci Maria Cristina Bona Giuseppe Ru Riccardo Orusa Fulvio Marsilio Vito Martella Barbara Di Martino |
spellingShingle |
Andrea Palombieri Serena Robetto Federica Di Profio Vittorio Sarchese Paola Fruci Maria Cristina Bona Giuseppe Ru Riccardo Orusa Fulvio Marsilio Vito Martella Barbara Di Martino Surveillance Study of Hepatitis E Virus (HEV) in Domestic and Wild Ruminants in Northwestern Italy Animals hepatitis E virus (HEV) domestic and wild ruminants HEV antibodies viral RNA. |
author_facet |
Andrea Palombieri Serena Robetto Federica Di Profio Vittorio Sarchese Paola Fruci Maria Cristina Bona Giuseppe Ru Riccardo Orusa Fulvio Marsilio Vito Martella Barbara Di Martino |
author_sort |
Andrea Palombieri |
title |
Surveillance Study of Hepatitis E Virus (HEV) in Domestic and Wild Ruminants in Northwestern Italy |
title_short |
Surveillance Study of Hepatitis E Virus (HEV) in Domestic and Wild Ruminants in Northwestern Italy |
title_full |
Surveillance Study of Hepatitis E Virus (HEV) in Domestic and Wild Ruminants in Northwestern Italy |
title_fullStr |
Surveillance Study of Hepatitis E Virus (HEV) in Domestic and Wild Ruminants in Northwestern Italy |
title_full_unstemmed |
Surveillance Study of Hepatitis E Virus (HEV) in Domestic and Wild Ruminants in Northwestern Italy |
title_sort |
surveillance study of hepatitis e virus (hev) in domestic and wild ruminants in northwestern italy |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2020-12-01 |
description |
In industrialized countries, increasing autochthonous infections of hepatitis E virus (HEV) are caused by zoonotic transmission of genotypes (Gts) 3 and 4, mainly through consumption of contaminated raw or undercooked pork meat. Although swine and wild boar are recognized as the main reservoir for Gt3 and Gt4, accumulating evidence indicates that other animal species, including domestic and wild ruminants, may harbor HEV. Herein, we screened molecularly and serologically serum and fecal samples from two domestic and four wild ruminant species collected in Valle d’Aosta and Piemonte regions (northwestern Italy. HEV antibodies were found in sheep (21.6%), goats (11.4%), red deer (2.6%), roe deer (3.1%), and in Alpine ibex (6.3%). Molecular screening was performed using different primer sets targeting highly conserved regions of hepeviruses and HEV RNA, although at low viral loads, was detected in four fecal specimens (3.0%, 4/134) collected from two HEV seropositive sheep herds. Taken together, the data obtained document the circulation of HEV in the geographical area assessed both in wild and domestic ruminants, but with the highest seroprevalence in sheep and goats. Consistently with results from other studies conducted in southern Italy, circulation of HEV among small domestic ruminants seems to occur more frequently than expected. |
topic |
hepatitis E virus (HEV) domestic and wild ruminants HEV antibodies viral RNA. |
url |
https://www.mdpi.com/2076-2615/10/12/2351 |
work_keys_str_mv |
AT andreapalombieri surveillancestudyofhepatitisevirushevindomesticandwildruminantsinnorthwesternitaly AT serenarobetto surveillancestudyofhepatitisevirushevindomesticandwildruminantsinnorthwesternitaly AT federicadiprofio surveillancestudyofhepatitisevirushevindomesticandwildruminantsinnorthwesternitaly AT vittoriosarchese surveillancestudyofhepatitisevirushevindomesticandwildruminantsinnorthwesternitaly AT paolafruci surveillancestudyofhepatitisevirushevindomesticandwildruminantsinnorthwesternitaly AT mariacristinabona surveillancestudyofhepatitisevirushevindomesticandwildruminantsinnorthwesternitaly AT giusepperu surveillancestudyofhepatitisevirushevindomesticandwildruminantsinnorthwesternitaly AT riccardoorusa surveillancestudyofhepatitisevirushevindomesticandwildruminantsinnorthwesternitaly AT fulviomarsilio surveillancestudyofhepatitisevirushevindomesticandwildruminantsinnorthwesternitaly AT vitomartella surveillancestudyofhepatitisevirushevindomesticandwildruminantsinnorthwesternitaly AT barbaradimartino surveillancestudyofhepatitisevirushevindomesticandwildruminantsinnorthwesternitaly |
_version_ |
1724387725823442944 |