Comparative Analysis of Milk Fat Globular Membrane (MFGM) Proteome between Saudi Arabia <i>Camelus dromedary</i> Safra and Wadha Breeds
Camel milk is traditionally known to have medicinal properties and many potential health benefits. Natural milk contains many soluble proteins and nanoparticles, such as a milk fat globule membrane (MFGM), a three-layered membrane covering of milk fat globule mainly composed of proteins and lipids,...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-05-01
|
Series: | Molecules |
Subjects: | |
Online Access: | https://www.mdpi.com/1420-3049/25/9/2146 |
id |
doaj-2cc7468e2add45f28864c17cc4a10c5d |
---|---|
record_format |
Article |
spelling |
doaj-2cc7468e2add45f28864c17cc4a10c5d2020-11-25T02:03:05ZengMDPI AGMolecules1420-30492020-05-01252146214610.3390/molecules25092146Comparative Analysis of Milk Fat Globular Membrane (MFGM) Proteome between Saudi Arabia <i>Camelus dromedary</i> Safra and Wadha BreedsBassam H. Sabha0Afshan Masood1Ibrahim O. Alanazi2Assim A. Alfadda3Hussein A. Almehdar4Hicham Benabdelkamel5Elrashdy M. Redwan6Department of Biological Science, Faculty of Science, King Abdulaziz University, Jeddah, P.O. Box 80203, Jeddah 21589, Saudi ArabiaProteomics Unit, Obesity Research Center, College of Medicine, King Saud University, P.O. Box 2925 (98), Riyadh 11461, Saudi ArabiaThe National Center for Genomic Technology (NCGT), Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), PO Box 6086, Riyadh 11461, Saudi ArabiaProteomics Unit, Obesity Research Center, College of Medicine, King Saud University, P.O. Box 2925 (98), Riyadh 11461, Saudi ArabiaDepartment of Biological Science, Faculty of Science, King Abdulaziz University, Jeddah, P.O. Box 80203, Jeddah 21589, Saudi ArabiaProteomics Unit, Obesity Research Center, College of Medicine, King Saud University, P.O. Box 2925 (98), Riyadh 11461, Saudi ArabiaDepartment of Biological Science, Faculty of Science, King Abdulaziz University, Jeddah, P.O. Box 80203, Jeddah 21589, Saudi ArabiaCamel milk is traditionally known to have medicinal properties and many potential health benefits. Natural milk contains many soluble proteins and nanoparticles, such as a milk fat globule membrane (MFGM), a three-layered membrane covering of milk fat globule mainly composed of proteins and lipids, which plays an important role in human health. MFGM proteins account for 1%–4% of total milk proteins, and their nutritive value and distribution depends on the different breeds. The differential composition of these membrane proteins among different camel breeds has not been explored. The current study, therefore, aimed to quantitatively analyze and compare the MFGM proteome between the milk produced by the two most common Saudi camel breeds, <i>Camelus dromedarius</i>: Safra and Wadha. Two-dimensional difference in gel electrophoresis (2D-DIGE) and mass spectrometry analysis revealed a total of 44 MFGM proteins that were identified with a significant difference in abundance (<i>p</i> ≤ 0.05; fold change ≥ 1.5) between the two breeds. Thirty-one proteins were up-regulated and 13 proteins were down-regulated in the Safra breed compared to the Wadha breed. The proteins identified with an increased abundance included α-lactalbumin, lactadherin, and annexin a8, whereas the down-regulated proteins included butyrophilin subfamily 1 member a1, lactotransferrin, and vinculin. The differentially abundant proteins were analyzed by the UNIPROT system and gene ontology (GO) to reveal their associations with known biological functions and pathways. Enzyme-linked immunosorbent assay (ELISA) confirmed the 2D-DIGE findings of butyrophilin (BTN) and α-lactalbumin (α-LA) levels obtained from Safra and Wadha breeds.https://www.mdpi.com/1420-3049/25/9/2146Safra breedWadha breedcamel milkproteomics2D-DIGEMFGM |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bassam H. Sabha Afshan Masood Ibrahim O. Alanazi Assim A. Alfadda Hussein A. Almehdar Hicham Benabdelkamel Elrashdy M. Redwan |
spellingShingle |
Bassam H. Sabha Afshan Masood Ibrahim O. Alanazi Assim A. Alfadda Hussein A. Almehdar Hicham Benabdelkamel Elrashdy M. Redwan Comparative Analysis of Milk Fat Globular Membrane (MFGM) Proteome between Saudi Arabia <i>Camelus dromedary</i> Safra and Wadha Breeds Molecules Safra breed Wadha breed camel milk proteomics 2D-DIGE MFGM |
author_facet |
Bassam H. Sabha Afshan Masood Ibrahim O. Alanazi Assim A. Alfadda Hussein A. Almehdar Hicham Benabdelkamel Elrashdy M. Redwan |
author_sort |
Bassam H. Sabha |
title |
Comparative Analysis of Milk Fat Globular Membrane (MFGM) Proteome between Saudi Arabia <i>Camelus dromedary</i> Safra and Wadha Breeds |
title_short |
Comparative Analysis of Milk Fat Globular Membrane (MFGM) Proteome between Saudi Arabia <i>Camelus dromedary</i> Safra and Wadha Breeds |
title_full |
Comparative Analysis of Milk Fat Globular Membrane (MFGM) Proteome between Saudi Arabia <i>Camelus dromedary</i> Safra and Wadha Breeds |
title_fullStr |
Comparative Analysis of Milk Fat Globular Membrane (MFGM) Proteome between Saudi Arabia <i>Camelus dromedary</i> Safra and Wadha Breeds |
title_full_unstemmed |
Comparative Analysis of Milk Fat Globular Membrane (MFGM) Proteome between Saudi Arabia <i>Camelus dromedary</i> Safra and Wadha Breeds |
title_sort |
comparative analysis of milk fat globular membrane (mfgm) proteome between saudi arabia <i>camelus dromedary</i> safra and wadha breeds |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-05-01 |
description |
Camel milk is traditionally known to have medicinal properties and many potential health benefits. Natural milk contains many soluble proteins and nanoparticles, such as a milk fat globule membrane (MFGM), a three-layered membrane covering of milk fat globule mainly composed of proteins and lipids, which plays an important role in human health. MFGM proteins account for 1%–4% of total milk proteins, and their nutritive value and distribution depends on the different breeds. The differential composition of these membrane proteins among different camel breeds has not been explored. The current study, therefore, aimed to quantitatively analyze and compare the MFGM proteome between the milk produced by the two most common Saudi camel breeds, <i>Camelus dromedarius</i>: Safra and Wadha. Two-dimensional difference in gel electrophoresis (2D-DIGE) and mass spectrometry analysis revealed a total of 44 MFGM proteins that were identified with a significant difference in abundance (<i>p</i> ≤ 0.05; fold change ≥ 1.5) between the two breeds. Thirty-one proteins were up-regulated and 13 proteins were down-regulated in the Safra breed compared to the Wadha breed. The proteins identified with an increased abundance included α-lactalbumin, lactadherin, and annexin a8, whereas the down-regulated proteins included butyrophilin subfamily 1 member a1, lactotransferrin, and vinculin. The differentially abundant proteins were analyzed by the UNIPROT system and gene ontology (GO) to reveal their associations with known biological functions and pathways. Enzyme-linked immunosorbent assay (ELISA) confirmed the 2D-DIGE findings of butyrophilin (BTN) and α-lactalbumin (α-LA) levels obtained from Safra and Wadha breeds. |
topic |
Safra breed Wadha breed camel milk proteomics 2D-DIGE MFGM |
url |
https://www.mdpi.com/1420-3049/25/9/2146 |
work_keys_str_mv |
AT bassamhsabha comparativeanalysisofmilkfatglobularmembranemfgmproteomebetweensaudiarabiaicamelusdromedaryisafraandwadhabreeds AT afshanmasood comparativeanalysisofmilkfatglobularmembranemfgmproteomebetweensaudiarabiaicamelusdromedaryisafraandwadhabreeds AT ibrahimoalanazi comparativeanalysisofmilkfatglobularmembranemfgmproteomebetweensaudiarabiaicamelusdromedaryisafraandwadhabreeds AT assimaalfadda comparativeanalysisofmilkfatglobularmembranemfgmproteomebetweensaudiarabiaicamelusdromedaryisafraandwadhabreeds AT husseinaalmehdar comparativeanalysisofmilkfatglobularmembranemfgmproteomebetweensaudiarabiaicamelusdromedaryisafraandwadhabreeds AT hichambenabdelkamel comparativeanalysisofmilkfatglobularmembranemfgmproteomebetweensaudiarabiaicamelusdromedaryisafraandwadhabreeds AT elrashdymredwan comparativeanalysisofmilkfatglobularmembranemfgmproteomebetweensaudiarabiaicamelusdromedaryisafraandwadhabreeds |
_version_ |
1724949606945521664 |