Summary: | In this study, a metabolomics approach was applied to screen and identify biomarkers potentially associated with the anti-osteoporotic effect of a novel sialoglycoprotein isolated from the eggs of Carassius auratus (Ca-SGP). The alterations in serum metabolome following the stimulation by either an OVX-induced osteoporosis or the Ca-SGP treatment were investigated using a UPLC/Q-TOF MS platform. Results showed significant differences in the metabolic profiles between the control and the model group, as well as between the model and the Ca-SGP treated group, as revealed by PLS-DA. Further, several metabolites such as xanthine, phosphatidylcholine/phosphatidyl ethanolamine (PC/PE), l-homocystine, oxidized glutathione, taurocholic acid, ceramide (d18:0/18:0) and saccharopine were found to be significantly altered during the progression of osteoporosis. Finally, with the recovery to normal levels upon the treatment with Ca-SGP, l-homocystine was suggested to be a potential biomarker related to the mechanism of anti-osteoporotic activity of Ca-SGP against estrogen deficiency induced bone loss by regulating gut microbiota.
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