Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice
In this study, a metabolomics approach was applied to screen and identify biomarkers potentially associated with the anti-osteoporotic effect of a novel sialoglycoprotein isolated from the eggs of Carassius auratus (Ca-SGP). The alterations in serum metabolome following the stimulation by either an...
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doaj-2cd58fe05751460eb080673bcf5bf7042021-04-30T07:17:12ZengElsevierJournal of Functional Foods1756-46462019-10-0161Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized miceQiping Zhan0Yufeng Dai1Fei Wang2Xiaoyuan Mai3Meng Fu4Peng Wang5Jingfeng Wang6College of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Corresponding authors at: College of Food Science and Engineering, Ocean University of China, No.5 Yushan Road, Qingdao, Shandong Province, 266003, China.College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Corresponding authors at: College of Food Science and Engineering, Ocean University of China, No.5 Yushan Road, Qingdao, Shandong Province, 266003, China.In this study, a metabolomics approach was applied to screen and identify biomarkers potentially associated with the anti-osteoporotic effect of a novel sialoglycoprotein isolated from the eggs of Carassius auratus (Ca-SGP). The alterations in serum metabolome following the stimulation by either an OVX-induced osteoporosis or the Ca-SGP treatment were investigated using a UPLC/Q-TOF MS platform. Results showed significant differences in the metabolic profiles between the control and the model group, as well as between the model and the Ca-SGP treated group, as revealed by PLS-DA. Further, several metabolites such as xanthine, phosphatidylcholine/phosphatidyl ethanolamine (PC/PE), l-homocystine, oxidized glutathione, taurocholic acid, ceramide (d18:0/18:0) and saccharopine were found to be significantly altered during the progression of osteoporosis. Finally, with the recovery to normal levels upon the treatment with Ca-SGP, l-homocystine was suggested to be a potential biomarker related to the mechanism of anti-osteoporotic activity of Ca-SGP against estrogen deficiency induced bone loss by regulating gut microbiota.http://www.sciencedirect.com/science/article/pii/S1756464619304384Sialoglycoprotein isolated from the eggs of Carassius auratusOsteoporosisEstrogen deficiencySerum metabolomicsl-homocystine |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qiping Zhan Yufeng Dai Fei Wang Xiaoyuan Mai Meng Fu Peng Wang Jingfeng Wang |
spellingShingle |
Qiping Zhan Yufeng Dai Fei Wang Xiaoyuan Mai Meng Fu Peng Wang Jingfeng Wang Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice Journal of Functional Foods Sialoglycoprotein isolated from the eggs of Carassius auratus Osteoporosis Estrogen deficiency Serum metabolomics l-homocystine |
author_facet |
Qiping Zhan Yufeng Dai Fei Wang Xiaoyuan Mai Meng Fu Peng Wang Jingfeng Wang |
author_sort |
Qiping Zhan |
title |
Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice |
title_short |
Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice |
title_full |
Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice |
title_fullStr |
Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice |
title_full_unstemmed |
Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice |
title_sort |
metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2019-10-01 |
description |
In this study, a metabolomics approach was applied to screen and identify biomarkers potentially associated with the anti-osteoporotic effect of a novel sialoglycoprotein isolated from the eggs of Carassius auratus (Ca-SGP). The alterations in serum metabolome following the stimulation by either an OVX-induced osteoporosis or the Ca-SGP treatment were investigated using a UPLC/Q-TOF MS platform. Results showed significant differences in the metabolic profiles between the control and the model group, as well as between the model and the Ca-SGP treated group, as revealed by PLS-DA. Further, several metabolites such as xanthine, phosphatidylcholine/phosphatidyl ethanolamine (PC/PE), l-homocystine, oxidized glutathione, taurocholic acid, ceramide (d18:0/18:0) and saccharopine were found to be significantly altered during the progression of osteoporosis. Finally, with the recovery to normal levels upon the treatment with Ca-SGP, l-homocystine was suggested to be a potential biomarker related to the mechanism of anti-osteoporotic activity of Ca-SGP against estrogen deficiency induced bone loss by regulating gut microbiota. |
topic |
Sialoglycoprotein isolated from the eggs of Carassius auratus Osteoporosis Estrogen deficiency Serum metabolomics l-homocystine |
url |
http://www.sciencedirect.com/science/article/pii/S1756464619304384 |
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