Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice

In this study, a metabolomics approach was applied to screen and identify biomarkers potentially associated with the anti-osteoporotic effect of a novel sialoglycoprotein isolated from the eggs of Carassius auratus (Ca-SGP). The alterations in serum metabolome following the stimulation by either an...

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Main Authors:	Qiping Zhan, Yufeng Dai, Fei Wang, Xiaoyuan Mai, Meng Fu, Peng Wang, Jingfeng Wang
Format:	Article
Language:	English
Published:	Elsevier 2019-10-01
Series:	Journal of Functional Foods
Subjects:	Sialoglycoprotein isolated from the eggs of Carassius auratus Osteoporosis Estrogen deficiency Serum metabolomics l-homocystine
Online Access:	http://www.sciencedirect.com/science/article/pii/S1756464619304384

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spelling	doaj-2cd58fe05751460eb080673bcf5bf7042021-04-30T07:17:12ZengElsevierJournal of Functional Foods1756-46462019-10-0161Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized miceQiping Zhan0Yufeng Dai1Fei Wang2Xiaoyuan Mai3Meng Fu4Peng Wang5Jingfeng Wang6College of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Corresponding authors at: College of Food Science and Engineering, Ocean University of China, No.5 Yushan Road, Qingdao, Shandong Province, 266003, China.College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Corresponding authors at: College of Food Science and Engineering, Ocean University of China, No.5 Yushan Road, Qingdao, Shandong Province, 266003, China.In this study, a metabolomics approach was applied to screen and identify biomarkers potentially associated with the anti-osteoporotic effect of a novel sialoglycoprotein isolated from the eggs of Carassius auratus (Ca-SGP). The alterations in serum metabolome following the stimulation by either an OVX-induced osteoporosis or the Ca-SGP treatment were investigated using a UPLC/Q-TOF MS platform. Results showed significant differences in the metabolic profiles between the control and the model group, as well as between the model and the Ca-SGP treated group, as revealed by PLS-DA. Further, several metabolites such as xanthine, phosphatidylcholine/phosphatidyl ethanolamine (PC/PE), l-homocystine, oxidized glutathione, taurocholic acid, ceramide (d18:0/18:0) and saccharopine were found to be significantly altered during the progression of osteoporosis. Finally, with the recovery to normal levels upon the treatment with Ca-SGP, l-homocystine was suggested to be a potential biomarker related to the mechanism of anti-osteoporotic activity of Ca-SGP against estrogen deficiency induced bone loss by regulating gut microbiota.http://www.sciencedirect.com/science/article/pii/S1756464619304384Sialoglycoprotein isolated from the eggs of Carassius auratusOsteoporosisEstrogen deficiencySerum metabolomicsl-homocystine
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Qiping Zhan Yufeng Dai Fei Wang Xiaoyuan Mai Meng Fu Peng Wang Jingfeng Wang
spellingShingle	Qiping Zhan Yufeng Dai Fei Wang Xiaoyuan Mai Meng Fu Peng Wang Jingfeng Wang Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice Journal of Functional Foods Sialoglycoprotein isolated from the eggs of Carassius auratus Osteoporosis Estrogen deficiency Serum metabolomics l-homocystine
author_facet	Qiping Zhan Yufeng Dai Fei Wang Xiaoyuan Mai Meng Fu Peng Wang Jingfeng Wang
author_sort	Qiping Zhan
title	Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice
title_short	Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice
title_full	Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice
title_fullStr	Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice
title_full_unstemmed	Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice
title_sort	metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice
publisher	Elsevier
series	Journal of Functional Foods
issn	1756-4646
publishDate	2019-10-01
description	In this study, a metabolomics approach was applied to screen and identify biomarkers potentially associated with the anti-osteoporotic effect of a novel sialoglycoprotein isolated from the eggs of Carassius auratus (Ca-SGP). The alterations in serum metabolome following the stimulation by either an OVX-induced osteoporosis or the Ca-SGP treatment were investigated using a UPLC/Q-TOF MS platform. Results showed significant differences in the metabolic profiles between the control and the model group, as well as between the model and the Ca-SGP treated group, as revealed by PLS-DA. Further, several metabolites such as xanthine, phosphatidylcholine/phosphatidyl ethanolamine (PC/PE), l-homocystine, oxidized glutathione, taurocholic acid, ceramide (d18:0/18:0) and saccharopine were found to be significantly altered during the progression of osteoporosis. Finally, with the recovery to normal levels upon the treatment with Ca-SGP, l-homocystine was suggested to be a potential biomarker related to the mechanism of anti-osteoporotic activity of Ca-SGP against estrogen deficiency induced bone loss by regulating gut microbiota.
topic	Sialoglycoprotein isolated from the eggs of Carassius auratus Osteoporosis Estrogen deficiency Serum metabolomics l-homocystine
url	http://www.sciencedirect.com/science/article/pii/S1756464619304384
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Metabonomic analysis in investigating the anti-osteoporotic effect of sialoglycoprotein isolated from eggs of carassius auratus on ovariectomized mice

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