Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates
<p>Abstract</p> <p>Background</p> <p>The role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. The aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for...
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doaj-2cfb5adb84ab49bc96c348bc3e62bc182020-11-25T01:19:28ZengBMCBMC Infectious Diseases1471-23342010-03-011014610.1186/1471-2334-10-46Spatial distribution and risk factors of Brucellosis in Iberian wild ungulatesde la Fuente JoséPrieto José MMarín Clara MRuiz-Fons FranciscoOleaga ÁlvaroAcevedo PelayoVicente JoaquínMartínez DavidRevilla Miguelde Miguel María JArnal MaricruzBoadella MarianaMuñoz Pilar MBarral MartaBarberán Montserratde Luco DanielBlasco José MGortázar Christian<p>Abstract</p> <p>Background</p> <p>The role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. The aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several Iberian wild ungulates.</p> <p>Methods</p> <p>A multi-species indirect immunosorbent assay (iELISA) using <it>Brucella </it>S-LPS antigen was developed. In several regions having brucellosis in livestock, individual serum samples were taken between 1999 and 2009 from 2,579 wild bovids, 6,448 wild cervids and4,454 Eurasian wild boar (<it>Sus scrofa</it>), and tested to assess brucellosis apparent prevalence. Strains isolated from wild boar were characterized to identify the presence of markers shared with the strains isolated from domestic pigs.</p> <p>Results</p> <p>Mean apparent prevalence below 0.5% was identified in chamois (<it>Rupicapra pyrenaica</it>), Iberian wild goat (<it>Capra pyrenaica</it>), and red deer (<it>Cervus elaphus</it>). Roe deer (<it>Capreolus capreolus</it>), fallow deer (<it>Dama dama</it>), mouflon (<it>Ovis aries</it>) and Barbary sheep (<it>Ammotragus lervia</it>) tested were seronegative. Only one red deer and one Iberian wild goat resulted positive in culture, isolating <it>B. abortus </it>biovar 1 and <it>B. melitensis </it>biovar 1, respectively. Apparent prevalence in wild boar ranged from 25% to 46% in the different regions studied, with the highest figures detected in South-Central Spain. The probability of wild boar being positive in the iELISA was also affected by age, age-by-sex interaction, sampling month, and the density of outdoor domestic pigs. A total of 104 bacterial isolates were obtained from wild boar, being all identified as <it>B. suis </it>biovar 2. DNA polymorphisms were similar to those found in domestic pigs.</p> <p>Conclusions</p> <p>In conclusion, brucellosis in wild boar is widespread in the Iberian Peninsula, thus representing an important threat for domestic pigs. By contrast, wild ruminants were not identified as a significant brucellosis reservoir for livestock.</p> http://www.biomedcentral.com/1471-2334/10/46 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
de la Fuente José Prieto José M Marín Clara M Ruiz-Fons Francisco Oleaga Álvaro Acevedo Pelayo Vicente Joaquín Martínez David Revilla Miguel de Miguel María J Arnal Maricruz Boadella Mariana Muñoz Pilar M Barral Marta Barberán Montserrat de Luco Daniel Blasco José M Gortázar Christian |
spellingShingle |
de la Fuente José Prieto José M Marín Clara M Ruiz-Fons Francisco Oleaga Álvaro Acevedo Pelayo Vicente Joaquín Martínez David Revilla Miguel de Miguel María J Arnal Maricruz Boadella Mariana Muñoz Pilar M Barral Marta Barberán Montserrat de Luco Daniel Blasco José M Gortázar Christian Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates BMC Infectious Diseases |
author_facet |
de la Fuente José Prieto José M Marín Clara M Ruiz-Fons Francisco Oleaga Álvaro Acevedo Pelayo Vicente Joaquín Martínez David Revilla Miguel de Miguel María J Arnal Maricruz Boadella Mariana Muñoz Pilar M Barral Marta Barberán Montserrat de Luco Daniel Blasco José M Gortázar Christian |
author_sort |
de la Fuente José |
title |
Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates |
title_short |
Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates |
title_full |
Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates |
title_fullStr |
Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates |
title_full_unstemmed |
Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates |
title_sort |
spatial distribution and risk factors of brucellosis in iberian wild ungulates |
publisher |
BMC |
series |
BMC Infectious Diseases |
issn |
1471-2334 |
publishDate |
2010-03-01 |
description |
<p>Abstract</p> <p>Background</p> <p>The role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. The aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several Iberian wild ungulates.</p> <p>Methods</p> <p>A multi-species indirect immunosorbent assay (iELISA) using <it>Brucella </it>S-LPS antigen was developed. In several regions having brucellosis in livestock, individual serum samples were taken between 1999 and 2009 from 2,579 wild bovids, 6,448 wild cervids and4,454 Eurasian wild boar (<it>Sus scrofa</it>), and tested to assess brucellosis apparent prevalence. Strains isolated from wild boar were characterized to identify the presence of markers shared with the strains isolated from domestic pigs.</p> <p>Results</p> <p>Mean apparent prevalence below 0.5% was identified in chamois (<it>Rupicapra pyrenaica</it>), Iberian wild goat (<it>Capra pyrenaica</it>), and red deer (<it>Cervus elaphus</it>). Roe deer (<it>Capreolus capreolus</it>), fallow deer (<it>Dama dama</it>), mouflon (<it>Ovis aries</it>) and Barbary sheep (<it>Ammotragus lervia</it>) tested were seronegative. Only one red deer and one Iberian wild goat resulted positive in culture, isolating <it>B. abortus </it>biovar 1 and <it>B. melitensis </it>biovar 1, respectively. Apparent prevalence in wild boar ranged from 25% to 46% in the different regions studied, with the highest figures detected in South-Central Spain. The probability of wild boar being positive in the iELISA was also affected by age, age-by-sex interaction, sampling month, and the density of outdoor domestic pigs. A total of 104 bacterial isolates were obtained from wild boar, being all identified as <it>B. suis </it>biovar 2. DNA polymorphisms were similar to those found in domestic pigs.</p> <p>Conclusions</p> <p>In conclusion, brucellosis in wild boar is widespread in the Iberian Peninsula, thus representing an important threat for domestic pigs. By contrast, wild ruminants were not identified as a significant brucellosis reservoir for livestock.</p> |
url |
http://www.biomedcentral.com/1471-2334/10/46 |
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