Determinação simultânea de topiramato, carbamazepina, fenitoína e fenobarbital em plasma empregando cromatografia a gás com detector de nitrogênio e fósforo

• Main Authors: Roberta Zilles Hahn, Olyr Celestino Kreutz, Marina Venzon Antunes, Rafael Linden, Juliana da Silva, Cléber Álvares da Silva
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2013-01-01
• Series: Química Nova
• Subjects: topiramate; gas chromatography nitrogen phosphorus detector; TMAH
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422013000500018&lng=en&tlng=en

Topiramate and the other frequently co-administered antiepileptic drugs carbamazepine, phenytoin and phenobarbital were determined in 100 µL plasma samples by gas chromatography with nitrogen phosphorus detection (GC-NPD), after a one-step liquid-liquid extraction with ethyl acetate, followed by flash methylation with trimethylphenylammonium hydroxide. Total chromatographic run time was 12.5 min. Intra-assay and inter-assay precision was 2.5-7.3% and 1.6-5.2%, respectively. Accuracy was 100.1-104.2%. The limit of quantitation was 1 µg mL-1 for all analytes, proving suitable for routine application in therapeutic drug monitoring of antiepileptic drugs.