AFM-Based Method for Measurement of Normal and Osteoarthritic Human Articular Cartilage Surface Roughness
In osteoarthrosis, pathological features of articular cartilage are associated with degeneration and nanomechanical changes. The aim of this paper is to show that indentation-atomic force microscopy can monitor wear-related biomechanical changes in the hip joint of patients with osteoarthritis. Fift...
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doaj-2d3243cdf5a045abb899778fcbdab7992020-11-25T03:01:17ZengMDPI AGMaterials1996-19442020-05-01132302230210.3390/ma13102302AFM-Based Method for Measurement of Normal and Osteoarthritic Human Articular Cartilage Surface RoughnessMikhail Ihnatouski0Jolanta Pauk1Dmitrij Karev2Boris Karev3Scientific and Research Department, Yanka Kupala State University of Grodno, Grodno, Ozheshko str., 22, 230023 Grodno, BelarusBiomedical Engineering Institute, Bialystok University of Technology, Wiejska 45A, 15-351 Bialystok, PolandDepartment of Traumatology, Orthopedics and Field Surgery, Grodno State Medical University, Gorkogo str. 80, 230009 Grodno, BelarusDepartment of Orthopedic and Traumatology, Grodno City Emergency Hospital, Sovietskih Pogranichnikov str., 115, 230027 Grodno, BelarusIn osteoarthrosis, pathological features of articular cartilage are associated with degeneration and nanomechanical changes. The aim of this paper is to show that indentation-atomic force microscopy can monitor wear-related biomechanical changes in the hip joint of patients with osteoarthritis. Fifty patients (N = 50), aged 40 to 65, were included in the study. The mechanical properties and the submicron surface morphology of hyaline cartilage were investigated using atomic force microscopy. Measurements of the roughness parameters of cartilage surfaces were performed, including the arithmetic average of absolute values (<i>Ra</i>), the maximum peak height (<i>Rp</i>), and the mean spacing between local peaks (<i>S</i>). The arithmetic mean of the absolute values of the height of healthy cartilage was 86 nm, while wear began at <i>Ra</i> = 73 nm. The maximum changes of values of the roughness parameters differed from the healthy ones by 71%, 80%, and 51% for <i>Ra</i>, <i>Rp</i>, and <i>S</i>, respectively. Young’s modulus for healthy cartilage surfaces ranged from 1.7 to 0.5 MPa. For the three stages of cartilage wear, Young’s modulus increased, and then it approached the maximum value and decreased. AFM seems to be a powerful tool for surface analysis of biological samples as it enables indentation measurements in addition to imaging.https://www.mdpi.com/1996-1944/13/10/2302atomic force microscopyhyaline cartilageosteoarthrosissurface roughness |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mikhail Ihnatouski Jolanta Pauk Dmitrij Karev Boris Karev |
spellingShingle |
Mikhail Ihnatouski Jolanta Pauk Dmitrij Karev Boris Karev AFM-Based Method for Measurement of Normal and Osteoarthritic Human Articular Cartilage Surface Roughness Materials atomic force microscopy hyaline cartilage osteoarthrosis surface roughness |
author_facet |
Mikhail Ihnatouski Jolanta Pauk Dmitrij Karev Boris Karev |
author_sort |
Mikhail Ihnatouski |
title |
AFM-Based Method for Measurement of Normal and Osteoarthritic Human Articular Cartilage Surface Roughness |
title_short |
AFM-Based Method for Measurement of Normal and Osteoarthritic Human Articular Cartilage Surface Roughness |
title_full |
AFM-Based Method for Measurement of Normal and Osteoarthritic Human Articular Cartilage Surface Roughness |
title_fullStr |
AFM-Based Method for Measurement of Normal and Osteoarthritic Human Articular Cartilage Surface Roughness |
title_full_unstemmed |
AFM-Based Method for Measurement of Normal and Osteoarthritic Human Articular Cartilage Surface Roughness |
title_sort |
afm-based method for measurement of normal and osteoarthritic human articular cartilage surface roughness |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2020-05-01 |
description |
In osteoarthrosis, pathological features of articular cartilage are associated with degeneration and nanomechanical changes. The aim of this paper is to show that indentation-atomic force microscopy can monitor wear-related biomechanical changes in the hip joint of patients with osteoarthritis. Fifty patients (N = 50), aged 40 to 65, were included in the study. The mechanical properties and the submicron surface morphology of hyaline cartilage were investigated using atomic force microscopy. Measurements of the roughness parameters of cartilage surfaces were performed, including the arithmetic average of absolute values (<i>Ra</i>), the maximum peak height (<i>Rp</i>), and the mean spacing between local peaks (<i>S</i>). The arithmetic mean of the absolute values of the height of healthy cartilage was 86 nm, while wear began at <i>Ra</i> = 73 nm. The maximum changes of values of the roughness parameters differed from the healthy ones by 71%, 80%, and 51% for <i>Ra</i>, <i>Rp</i>, and <i>S</i>, respectively. Young’s modulus for healthy cartilage surfaces ranged from 1.7 to 0.5 MPa. For the three stages of cartilage wear, Young’s modulus increased, and then it approached the maximum value and decreased. AFM seems to be a powerful tool for surface analysis of biological samples as it enables indentation measurements in addition to imaging. |
topic |
atomic force microscopy hyaline cartilage osteoarthrosis surface roughness |
url |
https://www.mdpi.com/1996-1944/13/10/2302 |
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