Characterization of a recombinant β-glucosidase of GH3 family from glucosinolate-metabolizing human gut bacterium Enterococcus casseliflavus CP1 for nitrile production
A recombinant β-glucosidase human gut bacterium capable of nitrile production from desulfo-glucosinolates was studied. The bgl4 gene (2,151 bp) from Enterococcus casseliflavus CP1 was cloned and overexpressed in Escherichia coli BL21(DE3) at 25 °C for 16 h in LB medium using 0.5 mM isopropyl β-D-1...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Prince of Songkla University
2020-06-01
|
Series: | Songklanakarin Journal of Science and Technology (SJST) |
Subjects: | |
Online Access: | https://rdo.psu.ac.th/sjstweb/journal/42-3/8.pdf |