The use of a synthetic antigen for the serological diagnosis of human trichinellosis
Hosts infected with Trichinella produce antibodies specific for an epitope common to the TSL-1 family antigens. This epitope contained uncommon terminal 3, 6-dideoxy-D-arabinohexose (so called tyvelose) residues. The disaccharide moiety was synthesized and an immunodiagnostic assay was developed, wh...
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doaj-2d39371463dd437c8825562b64cc51e32021-02-02T03:42:02ZengEDP SciencesParasite1252-607X1776-10422001-06-018S141S14310.1051/parasite/200108s2141parasite200108s2p141The use of a synthetic antigen for the serological diagnosis of human trichinellosisBruschi F.Moretti A.Wassom D.Piergili Fioretti D.Hosts infected with Trichinella produce antibodies specific for an epitope common to the TSL-1 family antigens. This epitope contained uncommon terminal 3, 6-dideoxy-D-arabinohexose (so called tyvelose) residues. The disaccharide moiety was synthesized and an immunodiagnostic assay was developed, which was specific and sensitive in swine trichinellosis. We aimed to verify the specificity and sensitivity of this immunodiagnostic test in human trichinellosis. 15 sera from normal subjects, 12 from patients with other parasitic diseases and 50 from trichinellosis patients were tested. Indirect enzyme linked immunosorbent assay (ELISA) for specific IgG and an amplified ELISA for specific IgE were performed using β-tyvelose-GalNAc-bovine serum albumin (BSA) disaccharide conjugate or T. spiralis muscle larvae excretory/secretory (E/S) products, as antigens. Neither control sera nor other parasitic infection sera resulted positive both for IgG and IgE when synthetic or E/S antigens were used. In trichinellosis patient sera, specific IgG were present in 100 % of cases, irrespective of the antigen used, but whereas specific IgE were detected in 78 % using E/S antigens, a 100% positivity rate was obtained, using the β-tyvelose- BSA conjugate.http://dx.doi.org/10.1051/parasite/200108s2141β-tyvelosehuman trichinellosislgGlgEdiagnosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bruschi F. Moretti A. Wassom D. Piergili Fioretti D. |
spellingShingle |
Bruschi F. Moretti A. Wassom D. Piergili Fioretti D. The use of a synthetic antigen for the serological diagnosis of human trichinellosis Parasite β-tyvelose human trichinellosis lgG lgE diagnosis |
author_facet |
Bruschi F. Moretti A. Wassom D. Piergili Fioretti D. |
author_sort |
Bruschi F. |
title |
The use of a synthetic antigen for the serological diagnosis of human trichinellosis |
title_short |
The use of a synthetic antigen for the serological diagnosis of human trichinellosis |
title_full |
The use of a synthetic antigen for the serological diagnosis of human trichinellosis |
title_fullStr |
The use of a synthetic antigen for the serological diagnosis of human trichinellosis |
title_full_unstemmed |
The use of a synthetic antigen for the serological diagnosis of human trichinellosis |
title_sort |
use of a synthetic antigen for the serological diagnosis of human trichinellosis |
publisher |
EDP Sciences |
series |
Parasite |
issn |
1252-607X 1776-1042 |
publishDate |
2001-06-01 |
description |
Hosts infected with Trichinella produce antibodies specific for an epitope common to the TSL-1 family antigens. This epitope contained uncommon terminal 3, 6-dideoxy-D-arabinohexose (so called tyvelose) residues. The disaccharide moiety was synthesized and an immunodiagnostic assay was developed, which was specific and sensitive in swine trichinellosis. We aimed to verify the specificity and sensitivity of this immunodiagnostic test in human trichinellosis. 15 sera from normal subjects, 12 from patients with other parasitic diseases and 50 from trichinellosis patients were tested. Indirect enzyme linked immunosorbent assay (ELISA) for specific IgG and an amplified ELISA for specific IgE were performed using β-tyvelose-GalNAc-bovine serum albumin (BSA) disaccharide conjugate or T. spiralis muscle larvae excretory/secretory (E/S) products, as antigens. Neither control sera nor other parasitic infection sera resulted positive both for IgG and IgE when synthetic or E/S antigens were used. In trichinellosis patient sera, specific IgG were present in 100 % of cases, irrespective of the antigen used, but whereas specific IgE were detected in 78 % using E/S antigens, a 100% positivity rate was obtained, using the β-tyvelose- BSA conjugate. |
topic |
β-tyvelose human trichinellosis lgG lgE diagnosis |
url |
http://dx.doi.org/10.1051/parasite/200108s2141 |
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