Thin-layer chromatography of several antihypertensive drugs from the group of angiotensin converting enzyme inhibitors

• Main Authors: ZIVOSLAV LJ. TESIC, DUSANKA M. MILOJKOVIC-OPSENICA, RADA M. BAOSIC, DANICA G. AGBABA, MIRJANA B. ALEKSIC
• Format: Article
• Language: English
• Published: Serbian Chemical Society 2001-01-01
• Series: Journal of the Serbian Chemical Society
• Subjects: thin-layer chromatography; silica gel; polyacrylonitrile sorbent; antihypertensive drugs; angiotensin converting enzyme inhibitors
• Online Access: http://www.shd.org.yu/HtDocs/SHD/Vol66/No1/V66-no1-06.pdf

A rapid and simple method for the chromatographic separation of pharmacologically active components contained in some antihypertensive drugs has been developed employing thin-layers of silica gel and polyacrylonitrile sorbent (PANS). The active compounds of Captopril (S)-1-(3-mercapto-2-methyl-1-oxopropyl)-L-proline, Enalapril (S)-1-[N- [1-(ethoxycarbonyl)-3-phenylpropyl]-L-alanyl]-L-proline, Lisinopril (S) -1-[N2-(carboxy-3-phenylpropyl)-L-lysyl]-L-proline, Quinapril [3S-[2[R*(R*)],3R*]], -2- [2-[[1(- etho- xycarbonyl)-3-phenylpropyl]amino]-1-oxopropyl]-1,2,3,4-tetrahydro-3-iso-quinoline-carboxylic acid, Ramipril [2S-[1[R*(R*)],2a3ab,6ab]]-1-[2[[1-(ethoxycarbo- nyl)-3-phenylpropyl]amino]-1-oxopropyl]octahydrocyclopenta[b]-pyrole-2-carboxylic acid and Cilazapril [1S-[1a,9a (R*)]]- 9-[[1-(ethoxycarbonyl)-3-phenylpropyl]amino] octahydro-10-oxo-6H-pyridazino[1,2-a][1,2] diazepine-1-carboxylic acid, were successfully separated by the presented procedures. For their chromatographic separation on silica gel sixteen and on PANS thirteen solvents were used.