Hypoxic preconditioning ameliorated neuronal injury after middle cerebral artery occlusion by promoting neurogenesis
Abstract Objectives Sequelae of stroke were mainly caused by neuronal injury. Oxygen is a key factor affecting the microenvironment of neural stem cells (NSCs), and oxygen levels are used to promote NSC neurogenesis. In this study, effects of intermittent hypoxic preconditioning (HPC) on neurogenesi...
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doaj-2d4416c0e7e5481c8080730b69265c1f2020-11-25T03:41:06ZengWileyBrain and Behavior2162-32792020-10-011010n/an/a10.1002/brb3.1804Hypoxic preconditioning ameliorated neuronal injury after middle cerebral artery occlusion by promoting neurogenesisLu Huang0Yaqi Wan1Zhancui Dang2Peng Yang3Quanyu Yang4Shizheng Wu5Research Center for High Altitude Medicine Qinghai University Xining ChinaQinghai Provincial People’s Hospital Xining ChinaQinghai University Medical College Xining ChinaQinghai Provincial People’s Hospital Xining ChinaQinghai University Medical College Xining ChinaQinghai Provincial People’s Hospital Xining ChinaAbstract Objectives Sequelae of stroke were mainly caused by neuronal injury. Oxygen is a key factor affecting the microenvironment of neural stem cells (NSCs), and oxygen levels are used to promote NSC neurogenesis. In this study, effects of intermittent hypoxic preconditioning (HPC) on neurogenesis were investigated in a rat model of middle cerebral artery occlusion (MCAO). Methods SD rats were used to establish the MCAO model. Nissl staining and Golgi staining were used to confirm the neuronal injury status in the MCAO model. Immunofluorescence, transmission electron microscopy, Western blot, and qPCR were used to observe the effects of HPC on neurogenesis. At the same time, the hypothesis that HPC could affect proliferation, apoptosis, differentiation, and migration of NSC was verified in vitro. Results Hypoxic preconditioning significantly ameliorated the neuronal injury induced by MCAO. Compared with MCAO group, the dendrites, Edu+/SOX2+, Edu+/DCX+, Edu+/NeuN+, Edu+/GFAP+, and Edu+/Tubulin+ positive cells in the HPC + MCAO group exhibited significantly difference. Similarly, axonal and other neuronal injuries in the HPC + MCAO group were also ameliorated. In the in vitro experiments, mild HPC significantly enhanced the viability of NSCs, promoted the migration of differentiated cells, and reduced apoptosis. Conclusions Our results showed that HPC significantly promotes neurogenesis after MCAO and ameliorates neuronal injury.https://doi.org/10.1002/brb3.1804intermittent hypoxic preconditionmiddle cerebral artery occlusionneural stem cellneurogenesisneuronal regenerationsubependymal ventricular zone |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lu Huang Yaqi Wan Zhancui Dang Peng Yang Quanyu Yang Shizheng Wu |
spellingShingle |
Lu Huang Yaqi Wan Zhancui Dang Peng Yang Quanyu Yang Shizheng Wu Hypoxic preconditioning ameliorated neuronal injury after middle cerebral artery occlusion by promoting neurogenesis Brain and Behavior intermittent hypoxic precondition middle cerebral artery occlusion neural stem cell neurogenesis neuronal regeneration subependymal ventricular zone |
author_facet |
Lu Huang Yaqi Wan Zhancui Dang Peng Yang Quanyu Yang Shizheng Wu |
author_sort |
Lu Huang |
title |
Hypoxic preconditioning ameliorated neuronal injury after middle cerebral artery occlusion by promoting neurogenesis |
title_short |
Hypoxic preconditioning ameliorated neuronal injury after middle cerebral artery occlusion by promoting neurogenesis |
title_full |
Hypoxic preconditioning ameliorated neuronal injury after middle cerebral artery occlusion by promoting neurogenesis |
title_fullStr |
Hypoxic preconditioning ameliorated neuronal injury after middle cerebral artery occlusion by promoting neurogenesis |
title_full_unstemmed |
Hypoxic preconditioning ameliorated neuronal injury after middle cerebral artery occlusion by promoting neurogenesis |
title_sort |
hypoxic preconditioning ameliorated neuronal injury after middle cerebral artery occlusion by promoting neurogenesis |
publisher |
Wiley |
series |
Brain and Behavior |
issn |
2162-3279 |
publishDate |
2020-10-01 |
description |
Abstract Objectives Sequelae of stroke were mainly caused by neuronal injury. Oxygen is a key factor affecting the microenvironment of neural stem cells (NSCs), and oxygen levels are used to promote NSC neurogenesis. In this study, effects of intermittent hypoxic preconditioning (HPC) on neurogenesis were investigated in a rat model of middle cerebral artery occlusion (MCAO). Methods SD rats were used to establish the MCAO model. Nissl staining and Golgi staining were used to confirm the neuronal injury status in the MCAO model. Immunofluorescence, transmission electron microscopy, Western blot, and qPCR were used to observe the effects of HPC on neurogenesis. At the same time, the hypothesis that HPC could affect proliferation, apoptosis, differentiation, and migration of NSC was verified in vitro. Results Hypoxic preconditioning significantly ameliorated the neuronal injury induced by MCAO. Compared with MCAO group, the dendrites, Edu+/SOX2+, Edu+/DCX+, Edu+/NeuN+, Edu+/GFAP+, and Edu+/Tubulin+ positive cells in the HPC + MCAO group exhibited significantly difference. Similarly, axonal and other neuronal injuries in the HPC + MCAO group were also ameliorated. In the in vitro experiments, mild HPC significantly enhanced the viability of NSCs, promoted the migration of differentiated cells, and reduced apoptosis. Conclusions Our results showed that HPC significantly promotes neurogenesis after MCAO and ameliorates neuronal injury. |
topic |
intermittent hypoxic precondition middle cerebral artery occlusion neural stem cell neurogenesis neuronal regeneration subependymal ventricular zone |
url |
https://doi.org/10.1002/brb3.1804 |
work_keys_str_mv |
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1724531690052780032 |