Essential Oil of Ocimum basilicum L. and (−)-Linalool Blocks the Excitability of Rat Sciatic Nerve
The racemate linalool and its levogyrus enantiomer [(−)-LIN] are present in many essential oils and possess several pharmacological activities, such as antinociceptive and anti-inflammatory. In this work, the effects of essential oil obtained from the cultivation of the Ocimum basilicum L. (EOOb) de...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2016-01-01
|
Series: | Evidence-Based Complementary and Alternative Medicine |
Online Access: | http://dx.doi.org/10.1155/2016/9012605 |
id |
doaj-2d51cc3f09a34bc9ad2d5952c02c4cf4 |
---|---|
record_format |
Article |
spelling |
doaj-2d51cc3f09a34bc9ad2d5952c02c4cf42020-11-24T22:49:14ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882016-01-01201610.1155/2016/90126059012605Essential Oil of Ocimum basilicum L. and (−)-Linalool Blocks the Excitability of Rat Sciatic NerveAntonio Medeiros Venancio0Francisco Walber Ferreira-da-Silva1Kerly Shamyra da Silva-Alves2Hugo de Carvalho Pimentel3Matheus Macêdo Lima4Michele Fraga de Santana5Péricles Barreto Alves6Givanildo Batista da Silva7José Henrique Leal-Cardoso8Murilo Marchioro9Physiology Department, Federal University of Sergipe, 49.100-000 São Cristóvão, SE, BrazilSuperior Institute of Biomedical Science, State University of Ceará, Campus of Itaperi, 60.740-000 Fortaleza, CE, BrazilSuperior Institute of Biomedical Science, State University of Ceará, Campus of Itaperi, 60.740-000 Fortaleza, CE, BrazilPhysiology Department, Federal University of Sergipe, 49.100-000 São Cristóvão, SE, BrazilPhysiology Department, Federal University of Sergipe, 49.100-000 São Cristóvão, SE, BrazilPhysiology Department, Federal University of Sergipe, 49.100-000 São Cristóvão, SE, BrazilChemistry Department, Federal University of Sergipe, 49.100-000 São Cristóvão, SE, BrazilChemistry Department, Federal University of Sergipe, 49.100-000 São Cristóvão, SE, BrazilSuperior Institute of Biomedical Science, State University of Ceará, Campus of Itaperi, 60.740-000 Fortaleza, CE, BrazilPhysiology Department, Federal University of Sergipe, 49.100-000 São Cristóvão, SE, BrazilThe racemate linalool and its levogyrus enantiomer [(−)-LIN] are present in many essential oils and possess several pharmacological activities, such as antinociceptive and anti-inflammatory. In this work, the effects of essential oil obtained from the cultivation of the Ocimum basilicum L. (EOOb) derived from Germplasm Bank rich in (−)-LIN content in the excitability of peripheral nervous system were studied. We used rat sciatic nerve to investigate the EOOb and (−)-LIN effects on neuron excitability and the extracellular recording technique was used to register the compound action potential (CAP). EOOb and (−)-LIN blocked the CAP in a concentration-dependent way and these effects were reversible after washout. EOOb blocked positive amplitude of 1st and 2nd CAP components with IC50 of 0.38±0.2 and 0.17±0.0 mg/mL, respectively. For (−)-LIN, these values were 0.23±0.0 and 0.13±0.0 mg/mL. Both components reduced the conduction velocity of CAP and the 2nd component seems to be more affected than the 1st component. In conclusion EOOb and (−)-LIN inhibited the excitability of peripheral nervous system in a similar way and potency, revealing that the effects of EOOb on excitability are due to the presence of (−)-LIN in the essential oil.http://dx.doi.org/10.1155/2016/9012605 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Antonio Medeiros Venancio Francisco Walber Ferreira-da-Silva Kerly Shamyra da Silva-Alves Hugo de Carvalho Pimentel Matheus Macêdo Lima Michele Fraga de Santana Péricles Barreto Alves Givanildo Batista da Silva José Henrique Leal-Cardoso Murilo Marchioro |
spellingShingle |
Antonio Medeiros Venancio Francisco Walber Ferreira-da-Silva Kerly Shamyra da Silva-Alves Hugo de Carvalho Pimentel Matheus Macêdo Lima Michele Fraga de Santana Péricles Barreto Alves Givanildo Batista da Silva José Henrique Leal-Cardoso Murilo Marchioro Essential Oil of Ocimum basilicum L. and (−)-Linalool Blocks the Excitability of Rat Sciatic Nerve Evidence-Based Complementary and Alternative Medicine |
author_facet |
Antonio Medeiros Venancio Francisco Walber Ferreira-da-Silva Kerly Shamyra da Silva-Alves Hugo de Carvalho Pimentel Matheus Macêdo Lima Michele Fraga de Santana Péricles Barreto Alves Givanildo Batista da Silva José Henrique Leal-Cardoso Murilo Marchioro |
author_sort |
Antonio Medeiros Venancio |
title |
Essential Oil of Ocimum basilicum L. and (−)-Linalool Blocks the Excitability of Rat Sciatic Nerve |
title_short |
Essential Oil of Ocimum basilicum L. and (−)-Linalool Blocks the Excitability of Rat Sciatic Nerve |
title_full |
Essential Oil of Ocimum basilicum L. and (−)-Linalool Blocks the Excitability of Rat Sciatic Nerve |
title_fullStr |
Essential Oil of Ocimum basilicum L. and (−)-Linalool Blocks the Excitability of Rat Sciatic Nerve |
title_full_unstemmed |
Essential Oil of Ocimum basilicum L. and (−)-Linalool Blocks the Excitability of Rat Sciatic Nerve |
title_sort |
essential oil of ocimum basilicum l. and (−)-linalool blocks the excitability of rat sciatic nerve |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2016-01-01 |
description |
The racemate linalool and its levogyrus enantiomer [(−)-LIN] are present in many essential oils and possess several pharmacological activities, such as antinociceptive and anti-inflammatory. In this work, the effects of essential oil obtained from the cultivation of the Ocimum basilicum L. (EOOb) derived from Germplasm Bank rich in (−)-LIN content in the excitability of peripheral nervous system were studied. We used rat sciatic nerve to investigate the EOOb and (−)-LIN effects on neuron excitability and the extracellular recording technique was used to register the compound action potential (CAP). EOOb and (−)-LIN blocked the CAP in a concentration-dependent way and these effects were reversible after washout. EOOb blocked positive amplitude of 1st and 2nd CAP components with IC50 of 0.38±0.2 and 0.17±0.0 mg/mL, respectively. For (−)-LIN, these values were 0.23±0.0 and 0.13±0.0 mg/mL. Both components reduced the conduction velocity of CAP and the 2nd component seems to be more affected than the 1st component. In conclusion EOOb and (−)-LIN inhibited the excitability of peripheral nervous system in a similar way and potency, revealing that the effects of EOOb on excitability are due to the presence of (−)-LIN in the essential oil. |
url |
http://dx.doi.org/10.1155/2016/9012605 |
work_keys_str_mv |
AT antoniomedeirosvenancio essentialoilofocimumbasilicumlandlinaloolblockstheexcitabilityofratsciaticnerve AT franciscowalberferreiradasilva essentialoilofocimumbasilicumlandlinaloolblockstheexcitabilityofratsciaticnerve AT kerlyshamyradasilvaalves essentialoilofocimumbasilicumlandlinaloolblockstheexcitabilityofratsciaticnerve AT hugodecarvalhopimentel essentialoilofocimumbasilicumlandlinaloolblockstheexcitabilityofratsciaticnerve AT matheusmacedolima essentialoilofocimumbasilicumlandlinaloolblockstheexcitabilityofratsciaticnerve AT michelefragadesantana essentialoilofocimumbasilicumlandlinaloolblockstheexcitabilityofratsciaticnerve AT periclesbarretoalves essentialoilofocimumbasilicumlandlinaloolblockstheexcitabilityofratsciaticnerve AT givanildobatistadasilva essentialoilofocimumbasilicumlandlinaloolblockstheexcitabilityofratsciaticnerve AT josehenriquelealcardoso essentialoilofocimumbasilicumlandlinaloolblockstheexcitabilityofratsciaticnerve AT murilomarchioro essentialoilofocimumbasilicumlandlinaloolblockstheexcitabilityofratsciaticnerve |
_version_ |
1725676615669645312 |