Fermentation Characteristics and Microbial Diversity of Tropical Grass-legumes Silages
Calliandra calothyrsus preserved in silage is an alternative method for improving the crude protein content of feeds for sustainable ruminant production. The aim of this research was to evaluate the quality of silage which contained different levels of C. calothyrsus by examining the fermentation ch...
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doaj-2d5478d95f7f4df0917adfb8ad5ae8182020-11-24T21:49:49ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172015-04-0128451151810.5713/ajas.14.062223121Fermentation Characteristics and Microbial Diversity of Tropical Grass-legumes SilagesRoni Ridwan0Iman Rusmana1Yantyati Widyastuti2Komang G. Wiryawan3Bambang Prasetya4Mitsuo Sakamoto5Moriya Ohkuma6 Study Program of Microbiology, Graduate School of Bogor Agricultural University, Campus IPB Darmaga Bogor, West Java 16680, Indonesia Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Campus IPB Darmaga Bogor, West Java 16680, .Indonesia Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong, West Java 16911, .Indonesia Department of Animal Nutrition and Feed Technology, Faculty of Animal Sciences, Bogor Agricultural University, Campus IPB Darmaga Bogor, West Java 16680, .Indonesia Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong, West Java 16911, .Indonesia Microbe Division/Japan Collection of Microorganisms RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, .Japan Microbe Division/Japan Collection of Microorganisms RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, .JapanCalliandra calothyrsus preserved in silage is an alternative method for improving the crude protein content of feeds for sustainable ruminant production. The aim of this research was to evaluate the quality of silage which contained different levels of C. calothyrsus by examining the fermentation characteristics and microbial diversity. Silage was made in a completely randomized design consisting of five treatments with three replications i.e.: R0, Pennisetum purpureum 100%; R1, P. purpureum 75%+C. calothyrsus 25%;, R2, P. purpureum 50%+C. calothyrsus 50%; R3, P. purpureum 25%+C. calothyrsus 75%; and R4, C. calothyrsus 100%. All silages were prepared using plastic jar silos (600 g) and incubated at room temperature for 30 days. Silages were analyzed for fermentation characteristics and microbial diversity. Increased levels of C. calothyrsus in silage had a significant effect (p<0.01) on the fermentation characteristics. The microbial diversity index decreased and activity was inhibited with increasing levels of C. calothyrsus. The microbial community indicated that there was a population of Lactobacillus plantarum, L. casei, L. brevis, Lactococcus lactis, Chryseobacterium sp., and uncultured bacteria. The result confirmed that silage with a combination of grass and C. calothyrsus had good fermentation characteristics and microbial communities were dominated by L. plantarum.http://www.ajas.info/upload/pdf/ajas-28-4-511.pdfSilageFermentation CharacteristicsDiversity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Roni Ridwan Iman Rusmana Yantyati Widyastuti Komang G. Wiryawan Bambang Prasetya Mitsuo Sakamoto Moriya Ohkuma |
spellingShingle |
Roni Ridwan Iman Rusmana Yantyati Widyastuti Komang G. Wiryawan Bambang Prasetya Mitsuo Sakamoto Moriya Ohkuma Fermentation Characteristics and Microbial Diversity of Tropical Grass-legumes Silages Asian-Australasian Journal of Animal Sciences Silage Fermentation Characteristics Diversity |
author_facet |
Roni Ridwan Iman Rusmana Yantyati Widyastuti Komang G. Wiryawan Bambang Prasetya Mitsuo Sakamoto Moriya Ohkuma |
author_sort |
Roni Ridwan |
title |
Fermentation Characteristics and Microbial Diversity of Tropical Grass-legumes Silages |
title_short |
Fermentation Characteristics and Microbial Diversity of Tropical Grass-legumes Silages |
title_full |
Fermentation Characteristics and Microbial Diversity of Tropical Grass-legumes Silages |
title_fullStr |
Fermentation Characteristics and Microbial Diversity of Tropical Grass-legumes Silages |
title_full_unstemmed |
Fermentation Characteristics and Microbial Diversity of Tropical Grass-legumes Silages |
title_sort |
fermentation characteristics and microbial diversity of tropical grass-legumes silages |
publisher |
Asian-Australasian Association of Animal Production Societies |
series |
Asian-Australasian Journal of Animal Sciences |
issn |
1011-2367 1976-5517 |
publishDate |
2015-04-01 |
description |
Calliandra calothyrsus preserved in silage is an alternative method for improving the crude protein content of feeds for sustainable ruminant production. The aim of this research was to evaluate the quality of silage which contained different levels of C. calothyrsus by examining the fermentation characteristics and microbial diversity. Silage was made in a completely randomized design consisting of five treatments with three replications i.e.: R0, Pennisetum purpureum 100%; R1, P. purpureum 75%+C. calothyrsus 25%;, R2, P. purpureum 50%+C. calothyrsus 50%; R3, P. purpureum 25%+C. calothyrsus 75%; and R4, C. calothyrsus 100%. All silages were prepared using plastic jar silos (600 g) and incubated at room temperature for 30 days. Silages were analyzed for fermentation characteristics and microbial diversity. Increased levels of C. calothyrsus in silage had a significant effect (p<0.01) on the fermentation characteristics. The microbial diversity index decreased and activity was inhibited with increasing levels of C. calothyrsus. The microbial community indicated that there was a population of Lactobacillus plantarum, L. casei, L. brevis, Lactococcus lactis, Chryseobacterium sp., and uncultured bacteria. The result confirmed that silage with a combination of grass and C. calothyrsus had good fermentation characteristics and microbial communities were dominated by L. plantarum. |
topic |
Silage Fermentation Characteristics Diversity |
url |
http://www.ajas.info/upload/pdf/ajas-28-4-511.pdf |
work_keys_str_mv |
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