Co-Administration of Curcumin and Bromocriptine Nano-liposomes for Induction of Apoptosis in Lung Cancer Cells
Background: In recent years, nanotechnology with modern advances in the macromolecular design of nano-carriers has proved to be helpful in the development of drugs delivery systems. This research represents a novel co-administration of nano-vehicles, known as liposomes. Liposomes efficiently encapsu...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Pasteur Institute of Iran
2020-01-01
|
Series: | Iranian Biomedical Journal |
Subjects: | |
Online Access: | http://ibj.pasteur.ac.ir/article-1-2858-en.html |
id |
doaj-2d577dec3b30458ea409ea0c65f23344 |
---|---|
record_format |
Article |
spelling |
doaj-2d577dec3b30458ea409ea0c65f233442020-11-25T02:05:43ZengPasteur Institute of IranIranian Biomedical Journal1028-852X2008-823X2020-01-012412429Co-Administration of Curcumin and Bromocriptine Nano-liposomes for Induction of Apoptosis in Lung Cancer CellsMojgan Sheikhpour0Majid Sadeghizadeh1Fatemeh Yazdian2Ali Mansoori3Hassan Asadi4Abolfazl Movafagh5Seyed Sadegh Shahraeini6 Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran Department of Medical Genetics, Cancer Research Center, Shohada Hospital School of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran Background: In recent years, nanotechnology with modern advances in the macromolecular design of nano-carriers has proved to be helpful in the development of drugs delivery systems. This research represents a novel co-administration of nano-vehicles, known as liposomes. Liposomes efficiently encapsulate curcumin and bromocriptine (BR) in a polymer structure, which results in enhanced aqueous solubility of the mentioned hydrophobic agents and higher bioavailability of the drugs. Methods: Preparation of curcumin and BR liposomes were carried out by the thin film method, and the amounts of purified drug and its release were analyzed. After dose determination, the human lung cancer cells (QU-DB) were exposed to BR and curcumin liposomes for 12, 24, and 48 h. Then the viability and apoptosis assays were carried out by using tetrazolium dye and flow cytometry technique, respectively. Results: In this research, in vitro anti-cancer effects of former nano-formulations on lung cancer cells was confirmed, and no cytotoxicity effects of these nano-preparations were observed in the normal cells (HFLF-PI5). Conclusion: Our findings suggest the nano-liposomal drugs as effective anti-cancer agents; however, additional clinical examinations are required.http://ibj.pasteur.ac.ir/article-1-2858-en.htmlapoptosisbromocriptinecurcumin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mojgan Sheikhpour Majid Sadeghizadeh Fatemeh Yazdian Ali Mansoori Hassan Asadi Abolfazl Movafagh Seyed Sadegh Shahraeini |
spellingShingle |
Mojgan Sheikhpour Majid Sadeghizadeh Fatemeh Yazdian Ali Mansoori Hassan Asadi Abolfazl Movafagh Seyed Sadegh Shahraeini Co-Administration of Curcumin and Bromocriptine Nano-liposomes for Induction of Apoptosis in Lung Cancer Cells Iranian Biomedical Journal apoptosis bromocriptine curcumin |
author_facet |
Mojgan Sheikhpour Majid Sadeghizadeh Fatemeh Yazdian Ali Mansoori Hassan Asadi Abolfazl Movafagh Seyed Sadegh Shahraeini |
author_sort |
Mojgan Sheikhpour |
title |
Co-Administration of Curcumin and Bromocriptine Nano-liposomes for Induction of Apoptosis in Lung Cancer Cells |
title_short |
Co-Administration of Curcumin and Bromocriptine Nano-liposomes for Induction of Apoptosis in Lung Cancer Cells |
title_full |
Co-Administration of Curcumin and Bromocriptine Nano-liposomes for Induction of Apoptosis in Lung Cancer Cells |
title_fullStr |
Co-Administration of Curcumin and Bromocriptine Nano-liposomes for Induction of Apoptosis in Lung Cancer Cells |
title_full_unstemmed |
Co-Administration of Curcumin and Bromocriptine Nano-liposomes for Induction of Apoptosis in Lung Cancer Cells |
title_sort |
co-administration of curcumin and bromocriptine nano-liposomes for induction of apoptosis in lung cancer cells |
publisher |
Pasteur Institute of Iran |
series |
Iranian Biomedical Journal |
issn |
1028-852X 2008-823X |
publishDate |
2020-01-01 |
description |
Background: In recent years, nanotechnology with modern advances in the macromolecular design of nano-carriers has proved to be helpful in the development of drugs delivery systems. This research represents a novel co-administration of nano-vehicles, known as liposomes. Liposomes efficiently encapsulate curcumin and bromocriptine (BR) in a polymer structure, which results in enhanced aqueous solubility of the mentioned hydrophobic agents and higher bioavailability of the drugs. Methods: Preparation of curcumin and BR liposomes were carried out by the thin film method, and the amounts of purified drug and its release were analyzed. After dose determination, the human lung cancer cells (QU-DB) were exposed to BR and curcumin liposomes for 12, 24, and 48 h. Then the viability and apoptosis assays were carried out by using tetrazolium dye and flow cytometry technique, respectively. Results: In this research, in vitro anti-cancer effects of former nano-formulations on lung cancer cells was confirmed, and no cytotoxicity effects of these nano-preparations were observed in the normal cells (HFLF-PI5). Conclusion: Our findings suggest the nano-liposomal drugs as effective anti-cancer agents; however, additional clinical examinations are required. |
topic |
apoptosis bromocriptine curcumin |
url |
http://ibj.pasteur.ac.ir/article-1-2858-en.html |
work_keys_str_mv |
AT mojgansheikhpour coadministrationofcurcuminandbromocriptinenanoliposomesforinductionofapoptosisinlungcancercells AT majidsadeghizadeh coadministrationofcurcuminandbromocriptinenanoliposomesforinductionofapoptosisinlungcancercells AT fatemehyazdian coadministrationofcurcuminandbromocriptinenanoliposomesforinductionofapoptosisinlungcancercells AT alimansoori coadministrationofcurcuminandbromocriptinenanoliposomesforinductionofapoptosisinlungcancercells AT hassanasadi coadministrationofcurcuminandbromocriptinenanoliposomesforinductionofapoptosisinlungcancercells AT abolfazlmovafagh coadministrationofcurcuminandbromocriptinenanoliposomesforinductionofapoptosisinlungcancercells AT seyedsadeghshahraeini coadministrationofcurcuminandbromocriptinenanoliposomesforinductionofapoptosisinlungcancercells |
_version_ |
1724937366603300864 |