Antitumor Effects of 5-Aminolevulinic Acid on Human Malignant Glioblastoma Cells
5-Aminolevulinic acid (5-ALA) is a naturally occurring non-proteinogenic amino acid, which contributes to the diagnosis and therapeutic approaches of various cancers, including glioblastoma (GBM). In the present study, we aimed to investigate whether 5-ALA exerted cytotoxic effects on GBM cells. We...
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doaj-2d665fe3c0e346e5b5c738f6f757089a2021-06-01T01:04:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-01225596559610.3390/ijms22115596Antitumor Effects of 5-Aminolevulinic Acid on Human Malignant Glioblastoma CellsMohammad Jalili-Nik0Farzaneh Abbasinezhad-moud1Sajad Sahab-Negah2Abolfazl Maghrouni3Mohammad Etezad Razavi4Maryam Khaleghi Ghadiri5Walter Stummer6Ali Gorji7Epilepsy Research Center, Westfälische Wilhelms-Universität, 48149 Münster, GermanyEpilepsy Research Center, Westfälische Wilhelms-Universität, 48149 Münster, GermanyNeuroscience Research Center, Mashhad University of Medical Sciences, Mashhad 9177948564, IranEpilepsy Research Center, Westfälische Wilhelms-Universität, 48149 Münster, GermanyOphthalmology, Eye Research Center, Mashhad University of Medical Sciences, Mashhad 9195965919, IranDepartment of Neurosurgery, Westfälische Wilhelms-Universität, 48149 Münster, GermanyDepartment of Neurosurgery, Westfälische Wilhelms-Universität, 48149 Münster, GermanyEpilepsy Research Center, Westfälische Wilhelms-Universität, 48149 Münster, Germany5-Aminolevulinic acid (5-ALA) is a naturally occurring non-proteinogenic amino acid, which contributes to the diagnosis and therapeutic approaches of various cancers, including glioblastoma (GBM). In the present study, we aimed to investigate whether 5-ALA exerted cytotoxic effects on GBM cells. We assessed cell viability, apoptosis rate, mRNA expressions of various apoptosis-related genes, generation of reactive oxygen species (ROS), and migration ability of the human U-87 malignant GBM cell line (U87MG) treated with 5-ALA at different doses. The half-maximal inhibitory concentration of 5-ALA on U87MG cells was 500 μg/mL after 7 days; 5-ALA was not toxic for human optic cells and NIH-3T3 cells at this concentration. The application of 5-ALA led to a significant increase in apoptotic cells, enhancement of Bax and p53 expressions, reduction in Bcl-2 expression, and an increase in ROS generation. Furthermore, the application of 5-ALA increased the accumulation of U87MG cells in the SUB-G1 population, decreased the expression of cyclin D1, and reduced the migration ability of U87MG cells. Our data indicate the potential cytotoxic effects of 5-ALA on U87MG cells. Further studies are required to determine the spectrum of the antitumor activity of 5-ALA on GBM.https://www.mdpi.com/1422-0067/22/11/5596brain tumorprotoporphyrincell deathapoptosistumor cell line |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohammad Jalili-Nik Farzaneh Abbasinezhad-moud Sajad Sahab-Negah Abolfazl Maghrouni Mohammad Etezad Razavi Maryam Khaleghi Ghadiri Walter Stummer Ali Gorji |
spellingShingle |
Mohammad Jalili-Nik Farzaneh Abbasinezhad-moud Sajad Sahab-Negah Abolfazl Maghrouni Mohammad Etezad Razavi Maryam Khaleghi Ghadiri Walter Stummer Ali Gorji Antitumor Effects of 5-Aminolevulinic Acid on Human Malignant Glioblastoma Cells International Journal of Molecular Sciences brain tumor protoporphyrin cell death apoptosis tumor cell line |
author_facet |
Mohammad Jalili-Nik Farzaneh Abbasinezhad-moud Sajad Sahab-Negah Abolfazl Maghrouni Mohammad Etezad Razavi Maryam Khaleghi Ghadiri Walter Stummer Ali Gorji |
author_sort |
Mohammad Jalili-Nik |
title |
Antitumor Effects of 5-Aminolevulinic Acid on Human Malignant Glioblastoma Cells |
title_short |
Antitumor Effects of 5-Aminolevulinic Acid on Human Malignant Glioblastoma Cells |
title_full |
Antitumor Effects of 5-Aminolevulinic Acid on Human Malignant Glioblastoma Cells |
title_fullStr |
Antitumor Effects of 5-Aminolevulinic Acid on Human Malignant Glioblastoma Cells |
title_full_unstemmed |
Antitumor Effects of 5-Aminolevulinic Acid on Human Malignant Glioblastoma Cells |
title_sort |
antitumor effects of 5-aminolevulinic acid on human malignant glioblastoma cells |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-05-01 |
description |
5-Aminolevulinic acid (5-ALA) is a naturally occurring non-proteinogenic amino acid, which contributes to the diagnosis and therapeutic approaches of various cancers, including glioblastoma (GBM). In the present study, we aimed to investigate whether 5-ALA exerted cytotoxic effects on GBM cells. We assessed cell viability, apoptosis rate, mRNA expressions of various apoptosis-related genes, generation of reactive oxygen species (ROS), and migration ability of the human U-87 malignant GBM cell line (U87MG) treated with 5-ALA at different doses. The half-maximal inhibitory concentration of 5-ALA on U87MG cells was 500 μg/mL after 7 days; 5-ALA was not toxic for human optic cells and NIH-3T3 cells at this concentration. The application of 5-ALA led to a significant increase in apoptotic cells, enhancement of Bax and p53 expressions, reduction in Bcl-2 expression, and an increase in ROS generation. Furthermore, the application of 5-ALA increased the accumulation of U87MG cells in the SUB-G1 population, decreased the expression of cyclin D1, and reduced the migration ability of U87MG cells. Our data indicate the potential cytotoxic effects of 5-ALA on U87MG cells. Further studies are required to determine the spectrum of the antitumor activity of 5-ALA on GBM. |
topic |
brain tumor protoporphyrin cell death apoptosis tumor cell line |
url |
https://www.mdpi.com/1422-0067/22/11/5596 |
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