The Role of Lipid Rafts in the Early Stage of Enterovirus 71 Infection
Background/Aims: Although it has been widely accepted that Enterovirus 71 (EV71) enters permissive cells via receptor-mediated endocytosis, the details of entry mechanism for EV71 still need more exploration. This study aimed to investigate the role of lipid rafts in the early stage of EV71 Infectio...
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Cell Physiol Biochem Press GmbH & Co KG
2015-02-01
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doaj-2d861eff9e7e449b9365550735c4afef2020-11-24T22:03:11ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782015-02-013541347135910.1159/000373956373956The Role of Lipid Rafts in the Early Stage of Enterovirus 71 InfectionYong-Zhe ZhuDa-Ge WuHao RenQing-Qiang XuKui-Cheng ZhengWei ChenSheng-Lin ChenXi-Jing QianQing-Yuan TaoYan WangPing ZhaoZhong-Tian QiBackground/Aims: Although it has been widely accepted that Enterovirus 71 (EV71) enters permissive cells via receptor-mediated endocytosis, the details of entry mechanism for EV71 still need more exploration. This study aimed to investigate the role of lipid rafts in the early stage of EV71 Infection. Methods: The effect of cholesterol depletion or addition of exogenous cholesterol was detected by immunofluorescence assays and quantitative real-time PCR. Effects of cholesterol depletion on the association of EV71 with lipid rafts were determined by flow cytometry and co-immunoprecipitation assays. Localization and internalization of EV71 and its receptor were assayed by confocal microscpoy and sucrose gradient analysis. The impact of cholesterol on the activation of phosphoinositide 3'-kinase/Akt signaling pathway during initial virus infection was analyzed by Western-blotting. Results: Disruption of membrane cholesterol by a pharmacological agent resulted in a significant reduction in the infectivity of EV71. The inhibitory effect could be reversed by the addition of exogenous cholesterol. Cholesterol depletion post-infection did not affect EV71 infection. While virus bound equally to cholesterol-depleted cells, EV71 particles failed to be internalized by cholesterol-depleted cells. EV71 capsid protein co-localized with cholera toxin B, a lipid-raft-dependent internalization marker. Conclusion: Lipid rafts play a critical role in virus endocytosis and in the activation of PI3K/Akt signaling pathway in the early stage of EV71 infection.http://www.karger.com/Article/FullText/373956Cell entryLipid raftsSignaling pathwayEnterovirus 71 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yong-Zhe Zhu Da-Ge Wu Hao Ren Qing-Qiang Xu Kui-Cheng Zheng Wei Chen Sheng-Lin Chen Xi-Jing Qian Qing-Yuan Tao Yan Wang Ping Zhao Zhong-Tian Qi |
spellingShingle |
Yong-Zhe Zhu Da-Ge Wu Hao Ren Qing-Qiang Xu Kui-Cheng Zheng Wei Chen Sheng-Lin Chen Xi-Jing Qian Qing-Yuan Tao Yan Wang Ping Zhao Zhong-Tian Qi The Role of Lipid Rafts in the Early Stage of Enterovirus 71 Infection Cellular Physiology and Biochemistry Cell entry Lipid rafts Signaling pathway Enterovirus 71 |
author_facet |
Yong-Zhe Zhu Da-Ge Wu Hao Ren Qing-Qiang Xu Kui-Cheng Zheng Wei Chen Sheng-Lin Chen Xi-Jing Qian Qing-Yuan Tao Yan Wang Ping Zhao Zhong-Tian Qi |
author_sort |
Yong-Zhe Zhu |
title |
The Role of Lipid Rafts in the Early Stage of Enterovirus 71 Infection |
title_short |
The Role of Lipid Rafts in the Early Stage of Enterovirus 71 Infection |
title_full |
The Role of Lipid Rafts in the Early Stage of Enterovirus 71 Infection |
title_fullStr |
The Role of Lipid Rafts in the Early Stage of Enterovirus 71 Infection |
title_full_unstemmed |
The Role of Lipid Rafts in the Early Stage of Enterovirus 71 Infection |
title_sort |
role of lipid rafts in the early stage of enterovirus 71 infection |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2015-02-01 |
description |
Background/Aims: Although it has been widely accepted that Enterovirus 71 (EV71) enters permissive cells via receptor-mediated endocytosis, the details of entry mechanism for EV71 still need more exploration. This study aimed to investigate the role of lipid rafts in the early stage of EV71 Infection. Methods: The effect of cholesterol depletion or addition of exogenous cholesterol was detected by immunofluorescence assays and quantitative real-time PCR. Effects of cholesterol depletion on the association of EV71 with lipid rafts were determined by flow cytometry and co-immunoprecipitation assays. Localization and internalization of EV71 and its receptor were assayed by confocal microscpoy and sucrose gradient analysis. The impact of cholesterol on the activation of phosphoinositide 3'-kinase/Akt signaling pathway during initial virus infection was analyzed by Western-blotting. Results: Disruption of membrane cholesterol by a pharmacological agent resulted in a significant reduction in the infectivity of EV71. The inhibitory effect could be reversed by the addition of exogenous cholesterol. Cholesterol depletion post-infection did not affect EV71 infection. While virus bound equally to cholesterol-depleted cells, EV71 particles failed to be internalized by cholesterol-depleted cells. EV71 capsid protein co-localized with cholera toxin B, a lipid-raft-dependent internalization marker. Conclusion: Lipid rafts play a critical role in virus endocytosis and in the activation of PI3K/Akt signaling pathway in the early stage of EV71 infection. |
topic |
Cell entry Lipid rafts Signaling pathway Enterovirus 71 |
url |
http://www.karger.com/Article/FullText/373956 |
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