Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensis
Abstract Background Anopheles lesteri and Anopheles sinensis are two major malaria vectors in China and Southeast Asia. They are dramatically different in terms of geographical distribution, host preference, resting habitats, and other traits associated with ecological adaptation and malaria transmi...
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doaj-2d97855a36f2483884c45e395fe147252020-11-24T21:54:20ZengBMCParasites & Vectors1756-33052016-11-019111010.1186/s13071-016-1855-0Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensisJiangtao Liang0Biao Cheng1Guoding Zhu2Yun Wei3Jianxia Tang4Jun Cao5Yajun Ma6Maria V. Sharakhova7Ai Xia8Igor V. Sharakhov9Department of Entomology, Nanjing Agricultural UniversityDepartment of Entomology, Nanjing Agricultural UniversityKey Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic DiseasesDepartment of Entomology, Nanjing Agricultural UniversityKey Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic DiseasesKey Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic DiseasesDepartment of Tropical Infectious Diseases, Faculty of Tropical Medicine and Public Health, Second Military Medical UniversityDepartment of Entomology, Fralin Life Science Institute, Virginia TechDepartment of Entomology, Nanjing Agricultural UniversityDepartment of Entomology, Fralin Life Science Institute, Virginia TechAbstract Background Anopheles lesteri and Anopheles sinensis are two major malaria vectors in China and Southeast Asia. They are dramatically different in terms of geographical distribution, host preference, resting habitats, and other traits associated with ecological adaptation and malaria transmission. Both species belong to the Anopheles hyrcanus group, but the extent of genetic differences between them is not well understood. To provide an effective way to differentiate between species and to find useful markers for population genetics studies, we performed a comparative cytogenetic analysis of these two malaria vectors. Results Presented here is a standard cytogenetic map for An. lesteri, and a comparative analysis of chromosome structure and gene order between An. lesteri and An. sinensis. Our results demonstrate that much of the gene order on chromosomes X and 2 was reshuffled between the two species. However, the banding pattern and the gene order on chromosome 3 appeared to be conserved. We also found two new polymorphic inversions, 2Lc and 3Rb, in An. lesteri, and we mapped the breakpoints of these two inversions on polytene chromosomes. Conclusions Our results demonstrate the extent of structural divergence of chromosomes between An. lesteri and An. sinensis, and provide a new taxonomic cytogenetic tool to distinguish between these two species. Polymorphic inversions of An. lesteri could serve as markers for studies of the population structure and ecological adaptations of this major malaria vector.http://link.springer.com/article/10.1186/s13071-016-1855-0Cytogenetic mapChromosomal inversionsAnopheles lesteriAnopheles sinensisGene orderArm homology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jiangtao Liang Biao Cheng Guoding Zhu Yun Wei Jianxia Tang Jun Cao Yajun Ma Maria V. Sharakhova Ai Xia Igor V. Sharakhov |
spellingShingle |
Jiangtao Liang Biao Cheng Guoding Zhu Yun Wei Jianxia Tang Jun Cao Yajun Ma Maria V. Sharakhova Ai Xia Igor V. Sharakhov Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensis Parasites & Vectors Cytogenetic map Chromosomal inversions Anopheles lesteri Anopheles sinensis Gene order Arm homology |
author_facet |
Jiangtao Liang Biao Cheng Guoding Zhu Yun Wei Jianxia Tang Jun Cao Yajun Ma Maria V. Sharakhova Ai Xia Igor V. Sharakhov |
author_sort |
Jiangtao Liang |
title |
Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensis |
title_short |
Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensis |
title_full |
Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensis |
title_fullStr |
Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensis |
title_full_unstemmed |
Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensis |
title_sort |
structural divergence of chromosomes between malaria vectors anopheles lesteri and anopheles sinensis |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2016-11-01 |
description |
Abstract Background Anopheles lesteri and Anopheles sinensis are two major malaria vectors in China and Southeast Asia. They are dramatically different in terms of geographical distribution, host preference, resting habitats, and other traits associated with ecological adaptation and malaria transmission. Both species belong to the Anopheles hyrcanus group, but the extent of genetic differences between them is not well understood. To provide an effective way to differentiate between species and to find useful markers for population genetics studies, we performed a comparative cytogenetic analysis of these two malaria vectors. Results Presented here is a standard cytogenetic map for An. lesteri, and a comparative analysis of chromosome structure and gene order between An. lesteri and An. sinensis. Our results demonstrate that much of the gene order on chromosomes X and 2 was reshuffled between the two species. However, the banding pattern and the gene order on chromosome 3 appeared to be conserved. We also found two new polymorphic inversions, 2Lc and 3Rb, in An. lesteri, and we mapped the breakpoints of these two inversions on polytene chromosomes. Conclusions Our results demonstrate the extent of structural divergence of chromosomes between An. lesteri and An. sinensis, and provide a new taxonomic cytogenetic tool to distinguish between these two species. Polymorphic inversions of An. lesteri could serve as markers for studies of the population structure and ecological adaptations of this major malaria vector. |
topic |
Cytogenetic map Chromosomal inversions Anopheles lesteri Anopheles sinensis Gene order Arm homology |
url |
http://link.springer.com/article/10.1186/s13071-016-1855-0 |
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