Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
The subfamily Triatominae (Hemiptera, Reduviidae) includes 150 species of blood-sucking insects, vectors of Chagas disease or American trypanosomiasis. Karyotypic information reveals a striking stability in the number of autosomes. However, this group shows substantial variability in genome size, th...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
|
Series: | Memórias do Instituto Oswaldo Cruz. |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016001000614&lng=en&tlng=en |
id |
doaj-2d98410a8dcd4afe8826bd9da4579d5d |
---|---|
record_format |
Article |
spelling |
doaj-2d98410a8dcd4afe8826bd9da4579d5d2020-11-24T22:39:32ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.1678-80601111061462410.1590/0074-02760160044S0074-02762016001000614Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)Vanessa Bellini BardellaSebastián PitaAndré Luis Laforga VanzelaCleber GalvãoFrancisco PanzeraThe subfamily Triatominae (Hemiptera, Reduviidae) includes 150 species of blood-sucking insects, vectors of Chagas disease or American trypanosomiasis. Karyotypic information reveals a striking stability in the number of autosomes. However, this group shows substantial variability in genome size, the amount and distribution of C-heterochromatin, and the chromosome positions of 45S rDNA clusters. Here, we analysed the karyotypes of 41 species from six different genera with C-fluorescence banding in order to evaluate the base-pair richness of heterochromatic regions. Our results show a high heterogeneity in the fluorescent staining of the heterochromatin in both autosomes and sex chromosomes, never reported before within an insect subfamily with holocentric chromosomes. This technique allows a clear discrimination of the heterochromatic regions classified as similar by C-banding, constituting a new chromosome marker with taxonomic and evolutionary significance. The diverse fluorescent patterns are likely due to the amplification of different repeated sequences, reflecting an unusual dynamic rearrangement in the genomes of this subfamily. Further, we discuss the evolution of these repeated sequences in both autosomes and sex chromosomes in species of Triatominae.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016001000614&lng=en&tlng=enHeteropteraTriatominaeholocentricchromosomesCMA/DAPI bandingrepetitive DNA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vanessa Bellini Bardella Sebastián Pita André Luis Laforga Vanzela Cleber Galvão Francisco Panzera |
spellingShingle |
Vanessa Bellini Bardella Sebastián Pita André Luis Laforga Vanzela Cleber Galvão Francisco Panzera Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae) Memórias do Instituto Oswaldo Cruz. Heteroptera Triatominae holocentric chromosomes CMA/DAPI banding repetitive DNA |
author_facet |
Vanessa Bellini Bardella Sebastián Pita André Luis Laforga Vanzela Cleber Galvão Francisco Panzera |
author_sort |
Vanessa Bellini Bardella |
title |
Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae) |
title_short |
Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae) |
title_full |
Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae) |
title_fullStr |
Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae) |
title_full_unstemmed |
Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae) |
title_sort |
heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (hemiptera, reduviidae) |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
1678-8060 |
description |
The subfamily Triatominae (Hemiptera, Reduviidae) includes 150 species of blood-sucking insects, vectors of Chagas disease or American trypanosomiasis. Karyotypic information reveals a striking stability in the number of autosomes. However, this group shows substantial variability in genome size, the amount and distribution of C-heterochromatin, and the chromosome positions of 45S rDNA clusters. Here, we analysed the karyotypes of 41 species from six different genera with C-fluorescence banding in order to evaluate the base-pair richness of heterochromatic regions. Our results show a high heterogeneity in the fluorescent staining of the heterochromatin in both autosomes and sex chromosomes, never reported before within an insect subfamily with holocentric chromosomes. This technique allows a clear discrimination of the heterochromatic regions classified as similar by C-banding, constituting a new chromosome marker with taxonomic and evolutionary significance. The diverse fluorescent patterns are likely due to the amplification of different repeated sequences, reflecting an unusual dynamic rearrangement in the genomes of this subfamily. Further, we discuss the evolution of these repeated sequences in both autosomes and sex chromosomes in species of Triatominae. |
topic |
Heteroptera Triatominae holocentric chromosomes CMA/DAPI banding repetitive DNA |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016001000614&lng=en&tlng=en |
work_keys_str_mv |
AT vanessabellinibardella heterochromatinbasepaircompositionanddiversificationinholocentricchromosomesofkissingbugshemipterareduviidae AT sebastianpita heterochromatinbasepaircompositionanddiversificationinholocentricchromosomesofkissingbugshemipterareduviidae AT andreluislaforgavanzela heterochromatinbasepaircompositionanddiversificationinholocentricchromosomesofkissingbugshemipterareduviidae AT clebergalvao heterochromatinbasepaircompositionanddiversificationinholocentricchromosomesofkissingbugshemipterareduviidae AT franciscopanzera heterochromatinbasepaircompositionanddiversificationinholocentricchromosomesofkissingbugshemipterareduviidae |
_version_ |
1725708437184053248 |