Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism.
The gut microbiota has been designated as an active regulator of glucose metabolism and metabolic phenotype in a number of animal and human observational studies. We evaluated the effect of removing as many bacteria as possible by antibiotics on postprandial physiology in healthy humans.Meal tests w...
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doaj-2da557644b0f4d5fa007103cbde2cbba2020-11-25T00:07:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011011e014235210.1371/journal.pone.0142352Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism.Kristian H MikkelsenMorten FrostMartin I BahlTine R LichtUlrich S JensenJacob RosenbergOluf PedersenTorben HansenJens F RehfeldJens J HolstTina VilsbøllFilip K KnopThe gut microbiota has been designated as an active regulator of glucose metabolism and metabolic phenotype in a number of animal and human observational studies. We evaluated the effect of removing as many bacteria as possible by antibiotics on postprandial physiology in healthy humans.Meal tests with measurements of postprandial glucose tolerance and postprandial release of insulin and gut hormones were performed before, immediately after and 6 weeks after a 4-day, broad-spectrum, per oral antibiotic cocktail (vancomycin 500 mg, gentamycin 40 mg and meropenem 500 mg once-daily) in a group of 12 lean and glucose tolerant males. Faecal samples were collected for culture-based assessment of changes in gut microbiota composition.Acute and dramatic reductions in the abundance of a representative set of gut bacteria was seen immediately following the antibiotic course, but no changes in postprandial glucose tolerance, insulin secretion or plasma lipid concentrations were found. Apart from an acute and reversible increase in peptide YY secretion, no changes were observed in postprandial gut hormone release.As evaluated by selective cultivation of gut bacteria, a broad-spectrum 4-day antibiotics course with vancomycin, gentamycin and meropenem induced shifts in gut microbiota composition that had no clinically relevant short or long-term effects on metabolic variables in healthy glucose-tolerant males.clinicaltrials.gov NCT01633762.http://europepmc.org/articles/PMC4643023?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kristian H Mikkelsen Morten Frost Martin I Bahl Tine R Licht Ulrich S Jensen Jacob Rosenberg Oluf Pedersen Torben Hansen Jens F Rehfeld Jens J Holst Tina Vilsbøll Filip K Knop |
spellingShingle |
Kristian H Mikkelsen Morten Frost Martin I Bahl Tine R Licht Ulrich S Jensen Jacob Rosenberg Oluf Pedersen Torben Hansen Jens F Rehfeld Jens J Holst Tina Vilsbøll Filip K Knop Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism. PLoS ONE |
author_facet |
Kristian H Mikkelsen Morten Frost Martin I Bahl Tine R Licht Ulrich S Jensen Jacob Rosenberg Oluf Pedersen Torben Hansen Jens F Rehfeld Jens J Holst Tina Vilsbøll Filip K Knop |
author_sort |
Kristian H Mikkelsen |
title |
Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism. |
title_short |
Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism. |
title_full |
Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism. |
title_fullStr |
Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism. |
title_full_unstemmed |
Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism. |
title_sort |
effect of antibiotics on gut microbiota, gut hormones and glucose metabolism. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
The gut microbiota has been designated as an active regulator of glucose metabolism and metabolic phenotype in a number of animal and human observational studies. We evaluated the effect of removing as many bacteria as possible by antibiotics on postprandial physiology in healthy humans.Meal tests with measurements of postprandial glucose tolerance and postprandial release of insulin and gut hormones were performed before, immediately after and 6 weeks after a 4-day, broad-spectrum, per oral antibiotic cocktail (vancomycin 500 mg, gentamycin 40 mg and meropenem 500 mg once-daily) in a group of 12 lean and glucose tolerant males. Faecal samples were collected for culture-based assessment of changes in gut microbiota composition.Acute and dramatic reductions in the abundance of a representative set of gut bacteria was seen immediately following the antibiotic course, but no changes in postprandial glucose tolerance, insulin secretion or plasma lipid concentrations were found. Apart from an acute and reversible increase in peptide YY secretion, no changes were observed in postprandial gut hormone release.As evaluated by selective cultivation of gut bacteria, a broad-spectrum 4-day antibiotics course with vancomycin, gentamycin and meropenem induced shifts in gut microbiota composition that had no clinically relevant short or long-term effects on metabolic variables in healthy glucose-tolerant males.clinicaltrials.gov NCT01633762. |
url |
http://europepmc.org/articles/PMC4643023?pdf=render |
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