Some parameters of innate immunity in senile group patients with eye injury

• Main Authors: I. K. Namazova, L. M. Akhmedova, S. R. Medjidova
• Format: Article
• Language: Russian
• Published: Ophthalmology Publishing Group 2014-07-01
• Series: Oftalʹmologiâ
• Subjects: eye injury; innate immunity; nk-cells; transferrin; phagocytosis; neutrophils
• Online Access: https://www.ophthalmojournal.com/opht/article/view/154

Purpose: Investigation of the parameters of innate immunity in senile group patients with eye injury.Methods: Standard ophthalmologic and immunologic study of 12 patients aged 63±11 with eye injury complicated by inflamma- tion. Immunological study of peripheral blood, tear and conjunctival scraping from the injured eye and the binate eye was carried out on 1-3 days after the injury. the parameters of innate local immunity was determined in the result of immune-cytological study of the conjunctival scraping by assessing phagocytosis of neutrophils and index of neutrophils/lymphocytes (INL), immunofluorescent counting of NK-cells CD56+ (panel of monoclonal antibodies of company «Sorbent» Ltd., Russia). the concentration of transferrin (a set of «Human» Company, Germany) in the blood and lacrimal fluid was assayed by the biochemical analyzer (Bioscreen MS-500). the results obtained were processed by the method of variational statistics.Results: As a result of the conducted studies there has been detected a decrease in the amount of transferrin both in blood and lacrimal fluid by almost 2 times (p <0,001). there was recorded the rise in the INL index at the systemic (3,8±0,1) and local (4.9±0.4) level, reflecting a compensatory, more pronounced activity of nonspecific protection factors of general and local immunity in the acute stage of inflammation. the level of phagocytosis in the blood of the examined patients was reduced relative to the control (p<0.001). In the scraping material of the injured eye the phagocytosis percentage was 16.4±0.9%. the results of a comparative study of the content of NK-cells (СD56+), revealed reduction in the average value of this parameter in the blood of the patients with eye injury relative to the control (p<0.001), and in the conjunctival scraping the level of СD56+-lymphocytes increased, amounting to 2-3 in the field of vision.Conclusion: In the senile group patients with an eye injury there is an activation of nonspecific defense factors, mainly, on the local level: neutrophilic infiltration of the mucosa of the conjunctiva, increase in INL on the average to 4.9, increase in the number of NK-cells. the sharp decrease in the concentration of transferrin in the systemic and local level can serve as an additional diagnostic criterion for inflammatory activity in eye trauma.