Investigation of regenerative and tiss ue-specific activity of tot al RNA of bone marrow cells

• Main Authors: Z. Z. Gonikova, A. O. Nikolskaya, L. A. Kirsanova, M. Yu. Shagidulin, N. A. Onishchenko, V. I. Sevastyanov
• Format: Article
• Language: Russian
• Published: Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov 2018-09-01
• Series: Vestnik Transplantologii i Iskusstvennyh Organov
• Subjects: bone marrow cells; rna; liver regeneration
• Online Access: https://journal.transpl.ru/vtio/article/view/913

Aim. To establish the ability of the total RNA extracted from the body’s bone marrow cells (BMCs), in which liver tissue was damaged, to serve as a carrier of targeted regenerative signals to this organ.Materials and methods. By method of adoptive transfer in rats (n = 37) the  mitotic and proliferative activity of liver and kidney cells were studied in intact  recipients after intraperitoneal injection: the mononuclear BMCs – 2,5×106;  5,0×106; 3,5×107 cells – group 1 and the total RNA of the same BMCs  (30μg/100g of weight) – group 2 from donors in 12 hours after 70–75% of  hepatectomy; in group 3 (control), a saline solution was injected. RNA from  BMCs was extracted by the method developed by the «Evrogen» firm (Russia) with the reagent Extract RNA.Results. In group 2 in 48 and 72 h. there was the increasing of mitotic and  proliferative cell activity in the liver, but not in the kidneys (control of the  specificity of regenerative signals); in group 1 there was no transfer of  regenerative signals to these organs.Conclusion. The authors believe that the total RNA from BMCs, activated by hepatectomy, accumulates targeted (hepatospecific) regeneration signals, but  they are perceived only when RNA has been obtained by the damaged tissue.