OPTIMIZATION OF EXTRACELLULAR TANNASE PRODUCTION BY ASPERGILLUS NIGER VAN TIEGHEM USING RESPONSE SURFACE METHODOLOGY
Response surface methodology (RSM) was used to optimize the production of tannase by a newly isolate of Aspergillus niger Van Tieghem using rotatable central composite design (RCCD). This statistical optimization process was carried out involving four of quantitative growth parameters (variables), n...
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Slovak University of Agriculture
2013-12-01
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doaj-2dc3e921d05943ff8e1cb3169cd6731a2020-11-25T00:34:54ZengSlovak University of AgricultureJournal of Microbiology, Biotechnology and Food Sciences1338-51782013-12-0133202207OPTIMIZATION OF EXTRACELLULAR TANNASE PRODUCTION BY ASPERGILLUS NIGER VAN TIEGHEM USING RESPONSE SURFACE METHODOLOGYHamada Abou-BakrMalak El-SahnAmr El-BannaResponse surface methodology (RSM) was used to optimize the production of tannase by a newly isolate of Aspergillus niger Van Tieghem using rotatable central composite design (RCCD). This statistical optimization process was carried out involving four of quantitative growth parameters (variables), namely tannic acid concentration, nitrogen source concentration, initial pH of the medium and inoculum size. A mathematical model expressing the production process of tannase by submerged fermentation (SmF) technique was generated statistically in the form of a second order polynomial equation. The model indicated the presence of significant linear, quadratic and interaction effects of the studied variables on tannase production by the fungal isolate. The results showed maximum tannase production (580 U/50 ml medium) at 2% tannic acid, 4 g/l sodium nitrate, pH 4 and inoculum size of 5×107 spores/50 ml medium, which was also verified by experimental data. http://www.jmbfs.org/wp-content/uploads/2013/11/jmbfs-0367-Abou-Bakr.pdfTannase optimizationresponse surface methodologyrotatable central composite designAspergillus niger Van Tieghemfungal tannin acyl hydrolase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hamada Abou-Bakr Malak El-Sahn Amr El-Banna |
spellingShingle |
Hamada Abou-Bakr Malak El-Sahn Amr El-Banna OPTIMIZATION OF EXTRACELLULAR TANNASE PRODUCTION BY ASPERGILLUS NIGER VAN TIEGHEM USING RESPONSE SURFACE METHODOLOGY Journal of Microbiology, Biotechnology and Food Sciences Tannase optimization response surface methodology rotatable central composite design Aspergillus niger Van Tieghem fungal tannin acyl hydrolase |
author_facet |
Hamada Abou-Bakr Malak El-Sahn Amr El-Banna |
author_sort |
Hamada Abou-Bakr |
title |
OPTIMIZATION OF EXTRACELLULAR TANNASE PRODUCTION BY ASPERGILLUS NIGER VAN TIEGHEM USING RESPONSE SURFACE METHODOLOGY |
title_short |
OPTIMIZATION OF EXTRACELLULAR TANNASE PRODUCTION BY ASPERGILLUS NIGER VAN TIEGHEM USING RESPONSE SURFACE METHODOLOGY |
title_full |
OPTIMIZATION OF EXTRACELLULAR TANNASE PRODUCTION BY ASPERGILLUS NIGER VAN TIEGHEM USING RESPONSE SURFACE METHODOLOGY |
title_fullStr |
OPTIMIZATION OF EXTRACELLULAR TANNASE PRODUCTION BY ASPERGILLUS NIGER VAN TIEGHEM USING RESPONSE SURFACE METHODOLOGY |
title_full_unstemmed |
OPTIMIZATION OF EXTRACELLULAR TANNASE PRODUCTION BY ASPERGILLUS NIGER VAN TIEGHEM USING RESPONSE SURFACE METHODOLOGY |
title_sort |
optimization of extracellular tannase production by aspergillus niger van tieghem using response surface methodology |
publisher |
Slovak University of Agriculture |
series |
Journal of Microbiology, Biotechnology and Food Sciences |
issn |
1338-5178 |
publishDate |
2013-12-01 |
description |
Response surface methodology (RSM) was used to optimize the production of tannase by a newly isolate of Aspergillus niger Van Tieghem using rotatable central composite design (RCCD). This statistical optimization process was carried out involving four of quantitative growth parameters (variables), namely tannic acid concentration, nitrogen source concentration, initial pH of the medium and inoculum size. A mathematical model expressing the production process of tannase by submerged fermentation (SmF) technique was generated statistically in the form of a second order polynomial equation. The model indicated the presence of significant linear, quadratic and interaction effects of the studied variables on tannase production by the fungal isolate. The results showed maximum tannase production (580 U/50 ml medium) at 2% tannic acid, 4 g/l sodium nitrate, pH 4 and inoculum size of 5×107 spores/50 ml medium, which was also verified by experimental data. |
topic |
Tannase optimization response surface methodology rotatable central composite design Aspergillus niger Van Tieghem fungal tannin acyl hydrolase |
url |
http://www.jmbfs.org/wp-content/uploads/2013/11/jmbfs-0367-Abou-Bakr.pdf |
work_keys_str_mv |
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