Glial cell line-derived neurotrophic factor and antioxidants preserve the electrical responsiveness of the spiral ganglion neurons after experimentally induced deafness
Cochlear implant surgery is currently the therapy of choice for profoundly deaf patients. However, the functionality of cochlear implants depends on the integrity of the auditory spiral ganglion neurons. This study assesses the combined efficacy of two classes of agents found effective in preventing...
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doaj-2dca84f7981743e1b75dd7e45d63cece2021-03-20T04:54:56ZengElsevierNeurobiology of Disease1095-953X2008-01-012911421Glial cell line-derived neurotrophic factor and antioxidants preserve the electrical responsiveness of the spiral ganglion neurons after experimentally induced deafnessJun Maruyama0Josef M. Miller1Mats Ulfendahl2Center for Hearing and Communication Research and Department of Clinical Neuroscience, Karolinska Institutet, Sweden; Department of Otolaryngology, Ehime University School of Medicine, Matsuyama, JapanCenter for Hearing and Communication Research and Department of Clinical Neuroscience, Karolinska Institutet, Sweden; Kresge Hearing Research Institute, University of Michigan, Ann Arbor, USACenter for Hearing and Communication Research and Department of Clinical Neuroscience, Karolinska Institutet, Sweden; Department of Otolaryngology, Karolinska University Hospital-Solna, Stockholm, Sweden; Corresponding author. Center for Hearing and Communication Research, Building M1, Karolinska University Hospital, Solna, SE-171 76 Stockholm, Sweden. Fax: +46 8 301876.Cochlear implant surgery is currently the therapy of choice for profoundly deaf patients. However, the functionality of cochlear implants depends on the integrity of the auditory spiral ganglion neurons. This study assesses the combined efficacy of two classes of agents found effective in preventing degeneration of the auditory nerve following deafness, neurotrophic factors, and antioxidants. Guinea pigs were deafened and treated for 4 weeks with either local administration of GDNF or a combination of GDNF and systemic injections of the antioxidants ascorbic acid and Trolox. The density of surviving spiral ganglion cells was significantly enhanced and the thresholds for eliciting an electrically evoked brain stem response were significantly reduced in GDNF treated animals compared to deafened-untreated. The addition of antioxidants significantly enhanced the evoked responsiveness over that observed with GDNF alone. The results suggest multiple sites of intervention in the rescue of these cells from deafferentation-induced cell death.http://www.sciencedirect.com/science/article/pii/S0969996107001726AntioxidantsGDNFSpiral ganglionCochleaInner earNeurotrophic factor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jun Maruyama Josef M. Miller Mats Ulfendahl |
spellingShingle |
Jun Maruyama Josef M. Miller Mats Ulfendahl Glial cell line-derived neurotrophic factor and antioxidants preserve the electrical responsiveness of the spiral ganglion neurons after experimentally induced deafness Neurobiology of Disease Antioxidants GDNF Spiral ganglion Cochlea Inner ear Neurotrophic factor |
author_facet |
Jun Maruyama Josef M. Miller Mats Ulfendahl |
author_sort |
Jun Maruyama |
title |
Glial cell line-derived neurotrophic factor and antioxidants preserve the electrical responsiveness of the spiral ganglion neurons after experimentally induced deafness |
title_short |
Glial cell line-derived neurotrophic factor and antioxidants preserve the electrical responsiveness of the spiral ganglion neurons after experimentally induced deafness |
title_full |
Glial cell line-derived neurotrophic factor and antioxidants preserve the electrical responsiveness of the spiral ganglion neurons after experimentally induced deafness |
title_fullStr |
Glial cell line-derived neurotrophic factor and antioxidants preserve the electrical responsiveness of the spiral ganglion neurons after experimentally induced deafness |
title_full_unstemmed |
Glial cell line-derived neurotrophic factor and antioxidants preserve the electrical responsiveness of the spiral ganglion neurons after experimentally induced deafness |
title_sort |
glial cell line-derived neurotrophic factor and antioxidants preserve the electrical responsiveness of the spiral ganglion neurons after experimentally induced deafness |
publisher |
Elsevier |
series |
Neurobiology of Disease |
issn |
1095-953X |
publishDate |
2008-01-01 |
description |
Cochlear implant surgery is currently the therapy of choice for profoundly deaf patients. However, the functionality of cochlear implants depends on the integrity of the auditory spiral ganglion neurons. This study assesses the combined efficacy of two classes of agents found effective in preventing degeneration of the auditory nerve following deafness, neurotrophic factors, and antioxidants. Guinea pigs were deafened and treated for 4 weeks with either local administration of GDNF or a combination of GDNF and systemic injections of the antioxidants ascorbic acid and Trolox. The density of surviving spiral ganglion cells was significantly enhanced and the thresholds for eliciting an electrically evoked brain stem response were significantly reduced in GDNF treated animals compared to deafened-untreated. The addition of antioxidants significantly enhanced the evoked responsiveness over that observed with GDNF alone. The results suggest multiple sites of intervention in the rescue of these cells from deafferentation-induced cell death. |
topic |
Antioxidants GDNF Spiral ganglion Cochlea Inner ear Neurotrophic factor |
url |
http://www.sciencedirect.com/science/article/pii/S0969996107001726 |
work_keys_str_mv |
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1724211761377181696 |