Identification of micro-RNA expression profile related to recurrence in women with ESMO low-risk endometrial cancer
Abstract Background Actual European pathological classification of early-stage endometrial cancer (EC) may show insufficient accuracy to precisely stratify recurrence risk, leading to potential over or under treatment. Micro-RNAs are post-transcriptional regulators involved in carcinogenic mechanism...
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doaj-2dd2c15192ec4bbd912c69940255a47b2020-11-25T02:08:39ZengBMCJournal of Translational Medicine1479-58762018-05-0116111310.1186/s12967-018-1515-6Identification of micro-RNA expression profile related to recurrence in women with ESMO low-risk endometrial cancerTiphaine de Foucher0Maria Sbeih1Jenifer Uzan2Sofiane Bendifallah3Marine Lefevre4Nathalie Chabbert-Buffet5Selim Aractingi6Catherine Uzan7Issam Abd Alsalam8Rana Mitri9Romain H. Fontaine10Emile Daraï11Bassam Haddad12Céline Méhats13Marcos Ballester14Geoffroy Canlorbe15Cyril Touboul16INSERM, UMR S 938, Sorbonne UniversityINSERM, UMR S 938, Sorbonne UniversityDepartment of Obstetrics and Gynaecology, Creteil Intercommunal Hospital, Université Paris Est, Paris XIIDepartment of Obstetrics and Gynaecology, Tenon University Hospital, Assistance Publique des Hôpitaux de Paris, Sorbonne UniversityDepartment of Pathology, Tenon University Hospital, Assistance Publique des Hôpitaux de Paris, Sorbonne UniversityINSERM, UMR S 938, Sorbonne UniversityINSERM, UMR S 938, Sorbonne UniversityDepartment of Surgery and Oncological Gynaecology, Pitié-Salpétrière University Hospital, Assistance Publique des Hôpitaux de Paris, Sorbonne UniversityDepartment of Pathology, Creteil Intercommunal Hospital, Université Paris Est, Paris XIIDepartment of Pathology, Creteil Intercommunal Hospital, Université Paris Est, Paris XIIINSERM, UMR S 938, Sorbonne UniversityINSERM, UMR S 938, Sorbonne UniversityDepartment of Obstetrics and Gynaecology, Creteil Intercommunal Hospital, Université Paris Est, Paris XIICochin Institute, Inserm U1016, CNRS 8104, University René DescartesINSERM, UMR S 938, Sorbonne UniversityINSERM, UMR S 938, Sorbonne UniversityDepartment of Obstetrics and Gynaecology, Creteil Intercommunal Hospital, Université Paris Est, Paris XIIAbstract Background Actual European pathological classification of early-stage endometrial cancer (EC) may show insufficient accuracy to precisely stratify recurrence risk, leading to potential over or under treatment. Micro-RNAs are post-transcriptional regulators involved in carcinogenic mechanisms, with some micro-RNA patterns of expression associated with EC characteristics and prognosis. We previously demonstrated that downregulation of micro-RNA-184 was associated with lymph node involvement in low-risk EC (LREC). The aim of this study was to evaluate whether micro-RNA signature in tumor tissues from LREC women can be correlated with the occurrence of recurrences. Methods MicroRNA expression was assessed by chip analysis and qRT-PCR in 7 formalin-fixed paraffin-embedded (FFPE) LREC primary tumors from women whose follow up showed recurrences (R+) and in 14 FFPE LREC primary tumors from women whose follow up did not show any recurrence (R−), matched for grade and age. Various statistical analyses, including enrichment analysis and a minimum p-value approach, were performed. Results The expression levels of micro-RNAs-184, -497-5p, and -196b-3p were significantly lower in R+ compared to R− women. Women with a micro-RNA-184 fold change < 0.083 were more likely to show recurrence (n = 6; 66%) compared to those with a micro-RNA-184 fold change > 0.083 (n = 1; 8%), p = 0.016. Women with a micro-RNA-196 fold change < 0.56 were more likely to show recurrence (n = 5; 100%) compared to those with a micro-RNA-196 fold change > 0.56 (n = 2; 13%), p = 0.001. Conclusions These findings confirm the great interest of micro-RNA-184 as a prognostic tool to improve the management of LREC women.http://link.springer.com/article/10.1186/s12967-018-1515-6Low risk endometrial cancerRecurrenceMicroRNAsMicroRNA-184 |
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language |
English |
format |
Article |
sources |
DOAJ |
author |
Tiphaine de Foucher Maria Sbeih Jenifer Uzan Sofiane Bendifallah Marine Lefevre Nathalie Chabbert-Buffet Selim Aractingi Catherine Uzan Issam Abd Alsalam Rana Mitri Romain H. Fontaine Emile Daraï Bassam Haddad Céline Méhats Marcos Ballester Geoffroy Canlorbe Cyril Touboul |
spellingShingle |
Tiphaine de Foucher Maria Sbeih Jenifer Uzan Sofiane Bendifallah Marine Lefevre Nathalie Chabbert-Buffet Selim Aractingi Catherine Uzan Issam Abd Alsalam Rana Mitri Romain H. Fontaine Emile Daraï Bassam Haddad Céline Méhats Marcos Ballester Geoffroy Canlorbe Cyril Touboul Identification of micro-RNA expression profile related to recurrence in women with ESMO low-risk endometrial cancer Journal of Translational Medicine Low risk endometrial cancer Recurrence MicroRNAs MicroRNA-184 |
author_facet |
Tiphaine de Foucher Maria Sbeih Jenifer Uzan Sofiane Bendifallah Marine Lefevre Nathalie Chabbert-Buffet Selim Aractingi Catherine Uzan Issam Abd Alsalam Rana Mitri Romain H. Fontaine Emile Daraï Bassam Haddad Céline Méhats Marcos Ballester Geoffroy Canlorbe Cyril Touboul |
author_sort |
Tiphaine de Foucher |
title |
Identification of micro-RNA expression profile related to recurrence in women with ESMO low-risk endometrial cancer |
title_short |
Identification of micro-RNA expression profile related to recurrence in women with ESMO low-risk endometrial cancer |
title_full |
Identification of micro-RNA expression profile related to recurrence in women with ESMO low-risk endometrial cancer |
title_fullStr |
Identification of micro-RNA expression profile related to recurrence in women with ESMO low-risk endometrial cancer |
title_full_unstemmed |
Identification of micro-RNA expression profile related to recurrence in women with ESMO low-risk endometrial cancer |
title_sort |
identification of micro-rna expression profile related to recurrence in women with esmo low-risk endometrial cancer |
publisher |
BMC |
series |
Journal of Translational Medicine |
issn |
1479-5876 |
publishDate |
2018-05-01 |
description |
Abstract Background Actual European pathological classification of early-stage endometrial cancer (EC) may show insufficient accuracy to precisely stratify recurrence risk, leading to potential over or under treatment. Micro-RNAs are post-transcriptional regulators involved in carcinogenic mechanisms, with some micro-RNA patterns of expression associated with EC characteristics and prognosis. We previously demonstrated that downregulation of micro-RNA-184 was associated with lymph node involvement in low-risk EC (LREC). The aim of this study was to evaluate whether micro-RNA signature in tumor tissues from LREC women can be correlated with the occurrence of recurrences. Methods MicroRNA expression was assessed by chip analysis and qRT-PCR in 7 formalin-fixed paraffin-embedded (FFPE) LREC primary tumors from women whose follow up showed recurrences (R+) and in 14 FFPE LREC primary tumors from women whose follow up did not show any recurrence (R−), matched for grade and age. Various statistical analyses, including enrichment analysis and a minimum p-value approach, were performed. Results The expression levels of micro-RNAs-184, -497-5p, and -196b-3p were significantly lower in R+ compared to R− women. Women with a micro-RNA-184 fold change < 0.083 were more likely to show recurrence (n = 6; 66%) compared to those with a micro-RNA-184 fold change > 0.083 (n = 1; 8%), p = 0.016. Women with a micro-RNA-196 fold change < 0.56 were more likely to show recurrence (n = 5; 100%) compared to those with a micro-RNA-196 fold change > 0.56 (n = 2; 13%), p = 0.001. Conclusions These findings confirm the great interest of micro-RNA-184 as a prognostic tool to improve the management of LREC women. |
topic |
Low risk endometrial cancer Recurrence MicroRNAs MicroRNA-184 |
url |
http://link.springer.com/article/10.1186/s12967-018-1515-6 |
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