Influence of pentoxifylline on gene expression of PAG1/ miR-1206/ SNHG14 in ischemic heart disease
The regulation by immune checkpoint is able to prevent excessive tissue damage caused by ischemia reperfusion (I/R); therefore, the study aims to investigate the behavior of phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1) mRNA, miR-1206 and small nucleolar RNA host ge...
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doaj-2dd903f328ac44719af8caa18c10d2c32021-02-11T04:22:33ZengElsevierBiochemistry and Biophysics Reports2405-58082021-03-0125100911Influence of pentoxifylline on gene expression of PAG1/ miR-1206/ SNHG14 in ischemic heart diseaseAhlam Abd el-Aziz0Mohamed Ali El-Desouky1Ayman Shafei2Mostafa Elnakib3Amr Mohamed Abdelmoniem4Department of Chemistry, Biochemistry Division, Faculty of Science, Cairo University, EgyptDepartment of Chemistry, Biochemistry Division, Faculty of Science, Cairo University, Egypt; Corresponding author. Department of Chemistry, Biochemistry Division, Faculty of Science, Cairo University, Cairo, Egypt.Military Medical Academy, Faculty of Medicine, Modern University for Technology and Information, Cairo, EgyptMedical Microbiology and Immunology, Community Service and Environmental Development, Armed Force Collage of Medicine, EgyptDepartment of Chemistry, Faculty of Science, Cairo University, P.O.12613, Giza, A.R., EgyptThe regulation by immune checkpoint is able to prevent excessive tissue damage caused by ischemia reperfusion (I/R); therefore, the study aims to investigate the behavior of phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1) mRNA, miR-1206 and small nucleolar RNA host gene 14 (SNHG14) during I/R and intake of pentoxifylline (PTX) as a protective drug. The relative expression level of PAG1/miR-1206/SNHG14 was determined by qRT-PCR. Cardiac tissue levels of cytotoxic T-lymphocyte associated antigen 4 (CTLA4) and PAG1 protein expression were determined by ELISA technique. The regulatory T cells achieved by the flow cytometry. The results found that the relative expression of SNHG14 was significantly upregulated in I/R, but suppressed in PTX treated groups with enhancement of the relative expression level of miR-1206. The gene and protein expression of PAG1 were downregulated with effective doses of PTX. The results showed that (30 and 40 mg/kg bwt) PTX dose suppressed the CTLA4 development significantly. The mean of the regulatory T cell in PTX protective groups is significantly reduced at (p < 0.001) in a comparison with I/R group. Spearman's correlation analysis revealed a significant negative correlation between SNHG14 and miR-1206, but a significant positive correlation between SNHG14 and PAG1 in I/R heart tissue. The results indicated that miR-1206 and SNHG14 can be used as biomarkers with perfect sensitivity and specificity. Using PTX reduced cardiac tissue damage. SNHG14 and miR-1206 can be used as a diagnostic tool in I/R.http://www.sciencedirect.com/science/article/pii/S2405580821000054PentoxifyllineIschemia reperfusionmiR-1206PAG1SNHG14 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ahlam Abd el-Aziz Mohamed Ali El-Desouky Ayman Shafei Mostafa Elnakib Amr Mohamed Abdelmoniem |
spellingShingle |
Ahlam Abd el-Aziz Mohamed Ali El-Desouky Ayman Shafei Mostafa Elnakib Amr Mohamed Abdelmoniem Influence of pentoxifylline on gene expression of PAG1/ miR-1206/ SNHG14 in ischemic heart disease Biochemistry and Biophysics Reports Pentoxifylline Ischemia reperfusion miR-1206 PAG1 SNHG14 |
author_facet |
Ahlam Abd el-Aziz Mohamed Ali El-Desouky Ayman Shafei Mostafa Elnakib Amr Mohamed Abdelmoniem |
author_sort |
Ahlam Abd el-Aziz |
title |
Influence of pentoxifylline on gene expression of PAG1/ miR-1206/ SNHG14 in ischemic heart disease |
title_short |
Influence of pentoxifylline on gene expression of PAG1/ miR-1206/ SNHG14 in ischemic heart disease |
title_full |
Influence of pentoxifylline on gene expression of PAG1/ miR-1206/ SNHG14 in ischemic heart disease |
title_fullStr |
Influence of pentoxifylline on gene expression of PAG1/ miR-1206/ SNHG14 in ischemic heart disease |
title_full_unstemmed |
Influence of pentoxifylline on gene expression of PAG1/ miR-1206/ SNHG14 in ischemic heart disease |
title_sort |
influence of pentoxifylline on gene expression of pag1/ mir-1206/ snhg14 in ischemic heart disease |
publisher |
Elsevier |
series |
Biochemistry and Biophysics Reports |
issn |
2405-5808 |
publishDate |
2021-03-01 |
description |
The regulation by immune checkpoint is able to prevent excessive tissue damage caused by ischemia reperfusion (I/R); therefore, the study aims to investigate the behavior of phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1) mRNA, miR-1206 and small nucleolar RNA host gene 14 (SNHG14) during I/R and intake of pentoxifylline (PTX) as a protective drug. The relative expression level of PAG1/miR-1206/SNHG14 was determined by qRT-PCR. Cardiac tissue levels of cytotoxic T-lymphocyte associated antigen 4 (CTLA4) and PAG1 protein expression were determined by ELISA technique. The regulatory T cells achieved by the flow cytometry. The results found that the relative expression of SNHG14 was significantly upregulated in I/R, but suppressed in PTX treated groups with enhancement of the relative expression level of miR-1206. The gene and protein expression of PAG1 were downregulated with effective doses of PTX. The results showed that (30 and 40 mg/kg bwt) PTX dose suppressed the CTLA4 development significantly. The mean of the regulatory T cell in PTX protective groups is significantly reduced at (p < 0.001) in a comparison with I/R group. Spearman's correlation analysis revealed a significant negative correlation between SNHG14 and miR-1206, but a significant positive correlation between SNHG14 and PAG1 in I/R heart tissue. The results indicated that miR-1206 and SNHG14 can be used as biomarkers with perfect sensitivity and specificity. Using PTX reduced cardiac tissue damage. SNHG14 and miR-1206 can be used as a diagnostic tool in I/R. |
topic |
Pentoxifylline Ischemia reperfusion miR-1206 PAG1 SNHG14 |
url |
http://www.sciencedirect.com/science/article/pii/S2405580821000054 |
work_keys_str_mv |
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