Clinical, histopathological, and molecular characterization of Mycoplasma species in sheep and goats in Egypt
Background and Aim: Mycoplasma infection in small ruminants is a serious problem in sheep and goat herds around the world. It is responsible for high economic losses and decreased animal productivity. This study aimed to highlight the clinical, histopathological, minimum inhibitory concentration (MI...
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Veterinary World
2021-09-01
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Online Access: | http://www.veterinaryworld.org/Vol.14/September-2021/33.pdf |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Walid S. Mousa Ahmed A. Zaghawa Ahmed M. Elsify Mohamed A. Nayel Zarroug H. Ibrahim Khalid A. Al-Kheraije Hesham R. Elhalfaway Dina El-Shafey Anis Anis Akram A. Salama |
spellingShingle |
Walid S. Mousa Ahmed A. Zaghawa Ahmed M. Elsify Mohamed A. Nayel Zarroug H. Ibrahim Khalid A. Al-Kheraije Hesham R. Elhalfaway Dina El-Shafey Anis Anis Akram A. Salama Clinical, histopathological, and molecular characterization of Mycoplasma species in sheep and goats in Egypt Veterinary World goats minimum inhibitory concentration mycoplasma polymerase chain reaction prevalence sheep |
author_facet |
Walid S. Mousa Ahmed A. Zaghawa Ahmed M. Elsify Mohamed A. Nayel Zarroug H. Ibrahim Khalid A. Al-Kheraije Hesham R. Elhalfaway Dina El-Shafey Anis Anis Akram A. Salama |
author_sort |
Walid S. Mousa |
title |
Clinical, histopathological, and molecular characterization of Mycoplasma species in sheep and goats in Egypt |
title_short |
Clinical, histopathological, and molecular characterization of Mycoplasma species in sheep and goats in Egypt |
title_full |
Clinical, histopathological, and molecular characterization of Mycoplasma species in sheep and goats in Egypt |
title_fullStr |
Clinical, histopathological, and molecular characterization of Mycoplasma species in sheep and goats in Egypt |
title_full_unstemmed |
Clinical, histopathological, and molecular characterization of Mycoplasma species in sheep and goats in Egypt |
title_sort |
clinical, histopathological, and molecular characterization of mycoplasma species in sheep and goats in egypt |
publisher |
Veterinary World |
series |
Veterinary World |
issn |
0972-8988 2231-0916 |
publishDate |
2021-09-01 |
description |
Background and Aim: Mycoplasma infection in small ruminants is a serious problem in sheep and goat herds around the world. It is responsible for high economic losses and decreased animal productivity. This study aimed to highlight the clinical, histopathological, minimum inhibitory concentration (MIC), and molecular characterization of Mycoplasma species in sheep and goats in Menoufiya Governorate, Egypt.
Materials and Methods: A total of 234 samples were collected; 104 samples were collected from pneumonic lung tissues from the abattoir, in addition, 10 and 20 samples collected from apparently and diseased sheep, respectively, and 40 and 60 samples were collected from apparently and diseased goats, respectively, which were subjected to isolation onto pleuropneumonia-like organism medium. Polymerase chain reaction (PCR), histopathological examination, and determination of the MIC were also performed.
Results: Of 104 samples of lung tissues showing pneumonic lesions, 56 (53.84%) were positive for Mycoplasma isolation. The positive isolation of Mycoplasma from 10 and 20 samples from apparently and diseased sheep was 30% and 40%, respectively as well as the positive isolation of Mycoplasma was 17% and 56.66% out of 40 and 60 apparently healthy and diseased field goat's cases, respectively. All the diseased sheep and goats showed respiratory manifestations, including cough, bilateral nasal discharge, conjunctivitis, and systemic reaction. Evaluation of the MIC for Mycoplasma ovipneumoniae revealed that lincospectin and tylosin were the most effective antibiotics at 2.5 μg/mL. Histopathological examination of affected lung tissue showed extensive hemorrhagic pneumonia with extensive alveolar hemorrhage. The PCR technique proved to be a rapid, specific, and sensitive method for the detection of M. ovipneumoniae and Mycoplasma arginini at 390 and 326 bp, respectively.
Conclusion: M. ovipneumoniae and M. arginini were the most prevalent species associated with respiratory infections in sheep and goats in the study area. Further studies are needed to investigate the role of these species in dissemination of the disease within herds of small ruminants. |
topic |
goats minimum inhibitory concentration mycoplasma polymerase chain reaction prevalence sheep |
url |
http://www.veterinaryworld.org/Vol.14/September-2021/33.pdf |
work_keys_str_mv |
AT walidsmousa clinicalhistopathologicalandmolecularcharacterizationofmycoplasmaspeciesinsheepandgoatsinegypt AT ahmedazaghawa clinicalhistopathologicalandmolecularcharacterizationofmycoplasmaspeciesinsheepandgoatsinegypt AT ahmedmelsify clinicalhistopathologicalandmolecularcharacterizationofmycoplasmaspeciesinsheepandgoatsinegypt AT mohamedanayel clinicalhistopathologicalandmolecularcharacterizationofmycoplasmaspeciesinsheepandgoatsinegypt AT zarroughibrahim clinicalhistopathologicalandmolecularcharacterizationofmycoplasmaspeciesinsheepandgoatsinegypt AT khalidaalkheraije clinicalhistopathologicalandmolecularcharacterizationofmycoplasmaspeciesinsheepandgoatsinegypt AT heshamrelhalfaway clinicalhistopathologicalandmolecularcharacterizationofmycoplasmaspeciesinsheepandgoatsinegypt AT dinaelshafey clinicalhistopathologicalandmolecularcharacterizationofmycoplasmaspeciesinsheepandgoatsinegypt AT anisanis clinicalhistopathologicalandmolecularcharacterizationofmycoplasmaspeciesinsheepandgoatsinegypt AT akramasalama clinicalhistopathologicalandmolecularcharacterizationofmycoplasmaspeciesinsheepandgoatsinegypt |
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doaj-2dda48bcd2624327b0731c99bfb395cc2021-09-28T07:03:27ZengVeterinary WorldVeterinary World0972-89882231-09162021-09-011492561256710.14202/vetworld.2021.2561-2567Clinical, histopathological, and molecular characterization of Mycoplasma species in sheep and goats in EgyptWalid S. Mousa0https://orcid.org/0000-0002-0168-6775Ahmed A. Zaghawa1https://orcid.org/0000-0002-3263-2948Ahmed M. Elsify2https://orcid.org/0000-0003-0660-9071Mohamed A. Nayel3https://orcid.org/0000-0001-9048-0746Zarroug H. Ibrahim4https://orcid.org/0000-0001-9386-1915Khalid A. Al-Kheraije5https://orcid.org/0000-0003-4966-0264Hesham R. Elhalfaway6Dina El-Shafey7Anis Anis8https://orcid.org/0000-0002-4552-6183Akram A. Salama9https://orcid.org/0000-0002-8047-8965Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, University of Sadat City, Egypt.Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, University of Sadat City, Egypt.Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, University of Sadat City, Egypt.Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, University of Sadat City, Egypt.Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah, Saudi Arabia; Department of Biomedical Sciences , College Veterinary Medicine, Sudan University of Science and Technology, Khartoum, Sudan.Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah, Saudi Arabia.Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, University of Sadat City, Egypt.Department of Mycoplasma, Animal Health Research Institute, Dokki, Giza, Egypt.Department of Pathology, Faculty of Veterinary Medicine, University of Sadat City, Egypt.Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, University of Sadat City, Egypt.Background and Aim: Mycoplasma infection in small ruminants is a serious problem in sheep and goat herds around the world. It is responsible for high economic losses and decreased animal productivity. This study aimed to highlight the clinical, histopathological, minimum inhibitory concentration (MIC), and molecular characterization of Mycoplasma species in sheep and goats in Menoufiya Governorate, Egypt. Materials and Methods: A total of 234 samples were collected; 104 samples were collected from pneumonic lung tissues from the abattoir, in addition, 10 and 20 samples collected from apparently and diseased sheep, respectively, and 40 and 60 samples were collected from apparently and diseased goats, respectively, which were subjected to isolation onto pleuropneumonia-like organism medium. Polymerase chain reaction (PCR), histopathological examination, and determination of the MIC were also performed. Results: Of 104 samples of lung tissues showing pneumonic lesions, 56 (53.84%) were positive for Mycoplasma isolation. The positive isolation of Mycoplasma from 10 and 20 samples from apparently and diseased sheep was 30% and 40%, respectively as well as the positive isolation of Mycoplasma was 17% and 56.66% out of 40 and 60 apparently healthy and diseased field goat's cases, respectively. All the diseased sheep and goats showed respiratory manifestations, including cough, bilateral nasal discharge, conjunctivitis, and systemic reaction. Evaluation of the MIC for Mycoplasma ovipneumoniae revealed that lincospectin and tylosin were the most effective antibiotics at 2.5 μg/mL. Histopathological examination of affected lung tissue showed extensive hemorrhagic pneumonia with extensive alveolar hemorrhage. The PCR technique proved to be a rapid, specific, and sensitive method for the detection of M. ovipneumoniae and Mycoplasma arginini at 390 and 326 bp, respectively. Conclusion: M. ovipneumoniae and M. arginini were the most prevalent species associated with respiratory infections in sheep and goats in the study area. Further studies are needed to investigate the role of these species in dissemination of the disease within herds of small ruminants.http://www.veterinaryworld.org/Vol.14/September-2021/33.pdfgoatsminimum inhibitory concentrationmycoplasmapolymerase chain reactionprevalencesheep |