Measurement of human leukocyte microsomal HMG-CoA reductase activity.

Measurement of human leukocyte microsomal HMG-CoA reductase activity.

Methods were developed for determination of microsomal HMG-CoA reductase activity from freshly isolated human lymphocytes, monocytes, and granulocytes or cultured human lymphoid cells. Reductase activity in monocytes is approximately twice that in lymphocytes or granulocytes. The activity in culture...

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Bibliographic Details
Main Authors: H J Harwood, Jr, M Schneider, P W Stacpoole
Format: Article
Language:English
Published: Elsevier 1984-09-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520377336
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spelling doaj-2e094e336e0d4b36bec52b5340a8cb952021-04-25T04:16:20ZengElsevierJournal of Lipid Research0022-22751984-09-01259967978Measurement of human leukocyte microsomal HMG-CoA reductase activity.H J Harwood, JrM SchneiderP W StacpooleMethods were developed for determination of microsomal HMG-CoA reductase activity from freshly isolated human lymphocytes, monocytes, and granulocytes or cultured human lymphoid cells. Reductase activity in monocytes is approximately twice that in lymphocytes or granulocytes. The activity in cultured cells is approximately 34-fold greater than that in freshly isolated cells. Assay conditions were such as to preclude formation of HMG-CoA cleavage products. Leukocyte reductase activity was inhibited by dichloroacetate, a noncompetitive inhibitor of rat liver reductase and a serum cholesterol-lowering agent in man. Measurement of microsomal reductase activity from freshly isolated leukocytes may prove useful in assessing in vivo regulation of cholesterol synthesis in man.http://www.sciencedirect.com/science/article/pii/S0022227520377336
collection DOAJ
language English
format Article
sources DOAJ
author H J Harwood, Jr
M Schneider
P W Stacpoole
spellingShingle H J Harwood, Jr
M Schneider
P W Stacpoole
Measurement of human leukocyte microsomal HMG-CoA reductase activity.
Journal of Lipid Research
author_facet H J Harwood, Jr
M Schneider
P W Stacpoole
author_sort H J Harwood, Jr
title Measurement of human leukocyte microsomal HMG-CoA reductase activity.
title_short Measurement of human leukocyte microsomal HMG-CoA reductase activity.
title_full Measurement of human leukocyte microsomal HMG-CoA reductase activity.
title_fullStr Measurement of human leukocyte microsomal HMG-CoA reductase activity.
title_full_unstemmed Measurement of human leukocyte microsomal HMG-CoA reductase activity.
title_sort measurement of human leukocyte microsomal hmg-coa reductase activity.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1984-09-01
description Methods were developed for determination of microsomal HMG-CoA reductase activity from freshly isolated human lymphocytes, monocytes, and granulocytes or cultured human lymphoid cells. Reductase activity in monocytes is approximately twice that in lymphocytes or granulocytes. The activity in cultured cells is approximately 34-fold greater than that in freshly isolated cells. Assay conditions were such as to preclude formation of HMG-CoA cleavage products. Leukocyte reductase activity was inhibited by dichloroacetate, a noncompetitive inhibitor of rat liver reductase and a serum cholesterol-lowering agent in man. Measurement of microsomal reductase activity from freshly isolated leukocytes may prove useful in assessing in vivo regulation of cholesterol synthesis in man.
url http://www.sciencedirect.com/science/article/pii/S0022227520377336
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AT mschneider measurementofhumanleukocytemicrosomalhmgcoareductaseactivity
AT pwstacpoole measurementofhumanleukocytemicrosomalhmgcoareductaseactivity
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