Antioxidant activity, antibacterial potential and characterization of active fraction of Dioscorea pentaphylla L. tuber extract collected from Similipal Biosphere Reserve, Odisha, India
ABSTRACT Dioscorea pentaphylla L., a wild tuber is used both as food and medicines among different ethnic groups of Similipal Biosphere Reserve, India. Tubers are used against skin infections. In order to establish and confirm tribal claims, methanol extract was subjected to fractionation. The activ...
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doaj-2e1a3ca20e4f457d909ef7cb58d64f582020-11-24T22:45:51ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902018-04-0153410.1590/s2175-97902017000417006S1984-82502017000400623Antioxidant activity, antibacterial potential and characterization of active fraction of Dioscorea pentaphylla L. tuber extract collected from Similipal Biosphere Reserve, Odisha, IndiaSanjeet KumarPadma MahantiNihar Ranjan SinghSakti Kant RathPadan Kumar JenaJayanta Kumar PatraABSTRACT Dioscorea pentaphylla L., a wild tuber is used both as food and medicines among different ethnic groups of Similipal Biosphere Reserve, India. Tubers are used against skin infections. In order to establish and confirm tribal claims, methanol extract was subjected to fractionation. The active fraction (DP1) was subsequently used for further purification and NMR (Nuclear magnetic resonance) characterization. The phytochemical analysis revealed the presence of saponin groups. The antibacterial activity of DP1 was done against selected bacterial strains (Salmonella typhi, Shigella flexneri, Streptococcus pyogenes, Streptococcus mutans and Vibrio cholerae) using DD (disc diffusion), AWD (agar well diffusion) and broth dilution assay. The activity was compared with antibiotics Penicillin and Kanamycin. It was observed that DP1 showed significant inhibitory activity against the tested bacteria. The characterization of DP1 through NMR analysis and presence of proton in carbon position at C-3, C-19, C-18, C-21 and C-27 was same as the known compound “Diosgenin”. Therefore, isolated compound was confirmed to be Diosgenin. The study for the first time showed that, diosgenin present in D. pentaphylla tuber was responsible for antibacterial and antioxidant potential. Present study highlights the importance of Dioscorea species as sources of diverse secondary metabolites for the isolation of active compound(s).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400623&lng=en&tlng=enDioscorea pentaphylla/extract/active fractionDioscorea pentaphylla/ antibacterial activity/antioxidantNMR characterization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sanjeet Kumar Padma Mahanti Nihar Ranjan Singh Sakti Kant Rath Padan Kumar Jena Jayanta Kumar Patra |
spellingShingle |
Sanjeet Kumar Padma Mahanti Nihar Ranjan Singh Sakti Kant Rath Padan Kumar Jena Jayanta Kumar Patra Antioxidant activity, antibacterial potential and characterization of active fraction of Dioscorea pentaphylla L. tuber extract collected from Similipal Biosphere Reserve, Odisha, India Brazilian Journal of Pharmaceutical Sciences Dioscorea pentaphylla/extract/active fraction Dioscorea pentaphylla/ antibacterial activity/antioxidant NMR characterization |
author_facet |
Sanjeet Kumar Padma Mahanti Nihar Ranjan Singh Sakti Kant Rath Padan Kumar Jena Jayanta Kumar Patra |
author_sort |
Sanjeet Kumar |
title |
Antioxidant activity, antibacterial potential and characterization of active fraction of Dioscorea pentaphylla L. tuber extract collected from Similipal Biosphere Reserve, Odisha, India |
title_short |
Antioxidant activity, antibacterial potential and characterization of active fraction of Dioscorea pentaphylla L. tuber extract collected from Similipal Biosphere Reserve, Odisha, India |
title_full |
Antioxidant activity, antibacterial potential and characterization of active fraction of Dioscorea pentaphylla L. tuber extract collected from Similipal Biosphere Reserve, Odisha, India |
title_fullStr |
Antioxidant activity, antibacterial potential and characterization of active fraction of Dioscorea pentaphylla L. tuber extract collected from Similipal Biosphere Reserve, Odisha, India |
title_full_unstemmed |
Antioxidant activity, antibacterial potential and characterization of active fraction of Dioscorea pentaphylla L. tuber extract collected from Similipal Biosphere Reserve, Odisha, India |
title_sort |
antioxidant activity, antibacterial potential and characterization of active fraction of dioscorea pentaphylla l. tuber extract collected from similipal biosphere reserve, odisha, india |
publisher |
Universidade de São Paulo |
series |
Brazilian Journal of Pharmaceutical Sciences |
issn |
2175-9790 |
publishDate |
2018-04-01 |
description |
ABSTRACT Dioscorea pentaphylla L., a wild tuber is used both as food and medicines among different ethnic groups of Similipal Biosphere Reserve, India. Tubers are used against skin infections. In order to establish and confirm tribal claims, methanol extract was subjected to fractionation. The active fraction (DP1) was subsequently used for further purification and NMR (Nuclear magnetic resonance) characterization. The phytochemical analysis revealed the presence of saponin groups. The antibacterial activity of DP1 was done against selected bacterial strains (Salmonella typhi, Shigella flexneri, Streptococcus pyogenes, Streptococcus mutans and Vibrio cholerae) using DD (disc diffusion), AWD (agar well diffusion) and broth dilution assay. The activity was compared with antibiotics Penicillin and Kanamycin. It was observed that DP1 showed significant inhibitory activity against the tested bacteria. The characterization of DP1 through NMR analysis and presence of proton in carbon position at C-3, C-19, C-18, C-21 and C-27 was same as the known compound “Diosgenin”. Therefore, isolated compound was confirmed to be Diosgenin. The study for the first time showed that, diosgenin present in D. pentaphylla tuber was responsible for antibacterial and antioxidant potential. Present study highlights the importance of Dioscorea species as sources of diverse secondary metabolites for the isolation of active compound(s). |
topic |
Dioscorea pentaphylla/extract/active fraction Dioscorea pentaphylla/ antibacterial activity/antioxidant NMR characterization |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400623&lng=en&tlng=en |
work_keys_str_mv |
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