Selenized Plant Oil Is an Efficient Source of Selenium for Selenoprotein Biosynthesis in Human Cell Lines
Selenium is an essential trace element which is incorporated in the form of a rare amino acid, the selenocysteine, into an important group of proteins, the selenoproteins. Among the twenty-five selenoprotein genes identified to date, several have important cellular functions in antioxidant defense,...
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doaj-2e1c6f8581634de5a0a472f04aa9eec02020-11-24T21:37:59ZengMDPI AGNutrients2072-66432019-07-01117152410.3390/nu11071524nu11071524Selenized Plant Oil Is an Efficient Source of Selenium for Selenoprotein Biosynthesis in Human Cell LinesJordan Sonet0Maurine Mosca1Katarzyna Bierla2Karolina Modzelewska3Anna Flis-Borsuk4Piotr Suchocki5Iza Ksiazek6Elzbieta Anuszewska7Anne-Laure Bulteau8Joanna Szpunar9Ryszard Lobinski10Laurent Chavatte11CNRS/UPPA, Institute of Analytical and Physical Chemistry for the Environment and Materials (IPREM), UMR5254, Hélioparc, F-64053 Pau, FranceCNRS/UPPA, Institute of Analytical and Physical Chemistry for the Environment and Materials (IPREM), UMR5254, Hélioparc, F-64053 Pau, FranceCNRS/UPPA, Institute of Analytical and Physical Chemistry for the Environment and Materials (IPREM), UMR5254, Hélioparc, F-64053 Pau, FranceCNRS/UPPA, Institute of Analytical and Physical Chemistry for the Environment and Materials (IPREM), UMR5254, Hélioparc, F-64053 Pau, FranceCNRS/UPPA, Institute of Analytical and Physical Chemistry for the Environment and Materials (IPREM), UMR5254, Hélioparc, F-64053 Pau, FranceDepartment of Bioanalysis and Analysis of Drugs, Medical University of Warsaw, Żwirki i Wigury 81, 02-091 Warszawa, PolandDepartment of Biochemistry and Biopharmaceuticals, National Medicines Institute, Chelmska 30/34, PL-00-725 Warszawa, PolandDepartment of Biochemistry and Biopharmaceuticals, National Medicines Institute, Chelmska 30/34, PL-00-725 Warszawa, PolandCNRS/UPPA, Institute of Analytical and Physical Chemistry for the Environment and Materials (IPREM), UMR5254, Hélioparc, F-64053 Pau, FranceCNRS/UPPA, Institute of Analytical and Physical Chemistry for the Environment and Materials (IPREM), UMR5254, Hélioparc, F-64053 Pau, FranceCNRS/UPPA, Institute of Analytical and Physical Chemistry for the Environment and Materials (IPREM), UMR5254, Hélioparc, F-64053 Pau, FranceCNRS/UPPA, Institute of Analytical and Physical Chemistry for the Environment and Materials (IPREM), UMR5254, Hélioparc, F-64053 Pau, FranceSelenium is an essential trace element which is incorporated in the form of a rare amino acid, the selenocysteine, into an important group of proteins, the selenoproteins. Among the twenty-five selenoprotein genes identified to date, several have important cellular functions in antioxidant defense, cell signaling and redox homeostasis. Many selenoproteins are regulated by the availability of selenium which mostly occurs in the form of water-soluble molecules, either organic (selenomethionine, selenocysteine, and selenoproteins) or inorganic (selenate or selenite). Recently, a mixture of selenitriglycerides, obtained by the reaction of selenite with sunflower oil at high temperature, referred to as Selol, was proposed as a novel non-toxic, highly bioavailable and active antioxidant and antineoplastic agent. Free selenite is not present in the final product since the two phases (water soluble and oil) are separated and the residual water-soluble selenite discarded. Here we compare the assimilation of selenium as Selol, selenite and selenate by various cancerous (LNCaP) or immortalized (HEK293 and PNT1A) cell lines. An approach combining analytical chemistry, molecular biology and biochemistry demonstrated that selenium from Selol was efficiently incorporated in selenoproteins in human cell lines, and thus produced the first ever evidence of the bioavailability of selenium from selenized lipids.https://www.mdpi.com/2072-6643/11/7/1524seleniumselenoproteinselenized lipidsSelolGpx1Gpx4Txnrd1Txnrd2ICP-MS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jordan Sonet Maurine Mosca Katarzyna Bierla Karolina Modzelewska Anna Flis-Borsuk Piotr Suchocki Iza Ksiazek Elzbieta Anuszewska Anne-Laure Bulteau Joanna Szpunar Ryszard Lobinski Laurent Chavatte |
spellingShingle |
Jordan Sonet Maurine Mosca Katarzyna Bierla Karolina Modzelewska Anna Flis-Borsuk Piotr Suchocki Iza Ksiazek Elzbieta Anuszewska Anne-Laure Bulteau Joanna Szpunar Ryszard Lobinski Laurent Chavatte Selenized Plant Oil Is an Efficient Source of Selenium for Selenoprotein Biosynthesis in Human Cell Lines Nutrients selenium selenoprotein selenized lipids Selol Gpx1 Gpx4 Txnrd1 Txnrd2 ICP-MS |
author_facet |
Jordan Sonet Maurine Mosca Katarzyna Bierla Karolina Modzelewska Anna Flis-Borsuk Piotr Suchocki Iza Ksiazek Elzbieta Anuszewska Anne-Laure Bulteau Joanna Szpunar Ryszard Lobinski Laurent Chavatte |
author_sort |
Jordan Sonet |
title |
Selenized Plant Oil Is an Efficient Source of Selenium for Selenoprotein Biosynthesis in Human Cell Lines |
title_short |
Selenized Plant Oil Is an Efficient Source of Selenium for Selenoprotein Biosynthesis in Human Cell Lines |
title_full |
Selenized Plant Oil Is an Efficient Source of Selenium for Selenoprotein Biosynthesis in Human Cell Lines |
title_fullStr |
Selenized Plant Oil Is an Efficient Source of Selenium for Selenoprotein Biosynthesis in Human Cell Lines |
title_full_unstemmed |
Selenized Plant Oil Is an Efficient Source of Selenium for Selenoprotein Biosynthesis in Human Cell Lines |
title_sort |
selenized plant oil is an efficient source of selenium for selenoprotein biosynthesis in human cell lines |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2019-07-01 |
description |
Selenium is an essential trace element which is incorporated in the form of a rare amino acid, the selenocysteine, into an important group of proteins, the selenoproteins. Among the twenty-five selenoprotein genes identified to date, several have important cellular functions in antioxidant defense, cell signaling and redox homeostasis. Many selenoproteins are regulated by the availability of selenium which mostly occurs in the form of water-soluble molecules, either organic (selenomethionine, selenocysteine, and selenoproteins) or inorganic (selenate or selenite). Recently, a mixture of selenitriglycerides, obtained by the reaction of selenite with sunflower oil at high temperature, referred to as Selol, was proposed as a novel non-toxic, highly bioavailable and active antioxidant and antineoplastic agent. Free selenite is not present in the final product since the two phases (water soluble and oil) are separated and the residual water-soluble selenite discarded. Here we compare the assimilation of selenium as Selol, selenite and selenate by various cancerous (LNCaP) or immortalized (HEK293 and PNT1A) cell lines. An approach combining analytical chemistry, molecular biology and biochemistry demonstrated that selenium from Selol was efficiently incorporated in selenoproteins in human cell lines, and thus produced the first ever evidence of the bioavailability of selenium from selenized lipids. |
topic |
selenium selenoprotein selenized lipids Selol Gpx1 Gpx4 Txnrd1 Txnrd2 ICP-MS |
url |
https://www.mdpi.com/2072-6643/11/7/1524 |
work_keys_str_mv |
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