Bushmeat Species Identification: Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow (LF) Strip for Identification of Formosan Reeves’ Muntjac (<i>Muntiacus reevesi micrurus</i>)
The identification of animal species of meat in meat products is of great concern for various reasons, such as public health, religious beliefs, food allergies, legal perspectives, and bushmeat control. In this study, we developed a new technique to identify Formosan Reeves’ muntjac in meat using re...
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doaj-2e20c33cb7a744e38c58199ba6ed4aab2021-02-08T00:00:51ZengMDPI AGAnimals2076-26152021-02-011142642610.3390/ani11020426Bushmeat Species Identification: Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow (LF) Strip for Identification of Formosan Reeves’ Muntjac (<i>Muntiacus reevesi micrurus</i>)Yun-Hsiu Hsu0Wei-Cheng Yang1Kun-Wei Chan2Department of Veterinary Medicine, College of Veterinary Medicine, National Chiayi University, Chiayi 60054, TaiwanDepartment of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei 10617, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chiayi University, Chiayi 60054, TaiwanThe identification of animal species of meat in meat products is of great concern for various reasons, such as public health, religious beliefs, food allergies, legal perspectives, and bushmeat control. In this study, we developed a new technique to identify Formosan Reeves’ muntjac in meat using recombinase polymerase amplification (RPA) in combination with a lateral flow (LF) strip. The DNA extracted from a piece of Formosan Reeves’ muntjac meat was amplified by a pair of specific primers based on its mitochondrial cytochrome b gene for 10 min at a constant temperature ranging from 30 to 45 °C using RPA. Using the specific probe added to the RPA reaction system, the amplified products were visualized on the LF strip within 5 min. The total operating time from quick DNA extraction to visualizing the result was approximately 30 min. The RPA-LF system we designed was efficient when using boiled, pan-fried, roasted, stir-fried, or stewed samples. The advantages of simple operation, speediness, and cost-effectiveness make our RPA-LF method a promising molecular detection tool for meat species identification of either raw or variously cooked Formosan Reeves’ muntjac meat. It is also possible to apply this method to identify the meat of other wildlife sources.https://www.mdpi.com/2076-2615/11/2/426bushmeatFormosan Reeves’ muntjacrecombinase polymerase amplificationlateral flow strip |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yun-Hsiu Hsu Wei-Cheng Yang Kun-Wei Chan |
spellingShingle |
Yun-Hsiu Hsu Wei-Cheng Yang Kun-Wei Chan Bushmeat Species Identification: Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow (LF) Strip for Identification of Formosan Reeves’ Muntjac (<i>Muntiacus reevesi micrurus</i>) Animals bushmeat Formosan Reeves’ muntjac recombinase polymerase amplification lateral flow strip |
author_facet |
Yun-Hsiu Hsu Wei-Cheng Yang Kun-Wei Chan |
author_sort |
Yun-Hsiu Hsu |
title |
Bushmeat Species Identification: Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow (LF) Strip for Identification of Formosan Reeves’ Muntjac (<i>Muntiacus reevesi micrurus</i>) |
title_short |
Bushmeat Species Identification: Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow (LF) Strip for Identification of Formosan Reeves’ Muntjac (<i>Muntiacus reevesi micrurus</i>) |
title_full |
Bushmeat Species Identification: Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow (LF) Strip for Identification of Formosan Reeves’ Muntjac (<i>Muntiacus reevesi micrurus</i>) |
title_fullStr |
Bushmeat Species Identification: Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow (LF) Strip for Identification of Formosan Reeves’ Muntjac (<i>Muntiacus reevesi micrurus</i>) |
title_full_unstemmed |
Bushmeat Species Identification: Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow (LF) Strip for Identification of Formosan Reeves’ Muntjac (<i>Muntiacus reevesi micrurus</i>) |
title_sort |
bushmeat species identification: recombinase polymerase amplification (rpa) combined with lateral flow (lf) strip for identification of formosan reeves’ muntjac (<i>muntiacus reevesi micrurus</i>) |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2021-02-01 |
description |
The identification of animal species of meat in meat products is of great concern for various reasons, such as public health, religious beliefs, food allergies, legal perspectives, and bushmeat control. In this study, we developed a new technique to identify Formosan Reeves’ muntjac in meat using recombinase polymerase amplification (RPA) in combination with a lateral flow (LF) strip. The DNA extracted from a piece of Formosan Reeves’ muntjac meat was amplified by a pair of specific primers based on its mitochondrial cytochrome b gene for 10 min at a constant temperature ranging from 30 to 45 °C using RPA. Using the specific probe added to the RPA reaction system, the amplified products were visualized on the LF strip within 5 min. The total operating time from quick DNA extraction to visualizing the result was approximately 30 min. The RPA-LF system we designed was efficient when using boiled, pan-fried, roasted, stir-fried, or stewed samples. The advantages of simple operation, speediness, and cost-effectiveness make our RPA-LF method a promising molecular detection tool for meat species identification of either raw or variously cooked Formosan Reeves’ muntjac meat. It is also possible to apply this method to identify the meat of other wildlife sources. |
topic |
bushmeat Formosan Reeves’ muntjac recombinase polymerase amplification lateral flow strip |
url |
https://www.mdpi.com/2076-2615/11/2/426 |
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