Proteolytic activity in the midgut of Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae), Pomegranate carob moth
In this study, the proteolytic activity in the midgut of Ectomyelois ceratoniae as the major pest of pomegranate was investigated to find nature of specific proteases and their properties for adopting possible pest management procedure. It was found that fourth and fifth instar larvae had the highes...
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University of Modena and Reggio Emilia
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doaj-2e3d6f2675524366a8e305f012150c412020-11-25T03:15:41ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2011-07-0182132142Proteolytic activity in the midgut of Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae), Pomegranate carob mothM RanjbarJJ SendiA ZibaeeIn this study, the proteolytic activity in the midgut of Ectomyelois ceratoniae as the major pest of pomegranate was investigated to find nature of specific proteases and their properties for adopting possible pest management procedure. It was found that fourth and fifth instar larvae had the highest proteolytic activity as well as specific proteinases including, elastase, trypsin-like, chymotrypsin-like and two exopeptidases. The optimal pH of general protease was 10 and 9 for azocasein, casein and hemoglobin as substrate. The optimal temperature of the total proteolytic activity in the midgut of E. ceratoniae was found 30 and 35 °C by using azocasein and casein as substrates, respectively. In case of hemoglobin, the enzyme showed the highest enzymatic activity at temperatures from 15 to 35 °C. There was no enhancement in the proteolytic activity by using different cations but SDS, citric acid and mercaptoetahnol significantly decreased the proteolytic activity in the midgut of E. ceratoniae. Using specific proteolytic inhibitors including PMSF, TLCK, TPCK, E-64, DTT and phenanthroline revealed presence of serine proteases as the major proteases in the midgut of E. ceratoniae.http://www.isj.unimo.it/articoli/ISJ238.pdfEctomyelois ceratoniaemidgutproteasecharacterization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M Ranjbar JJ Sendi A Zibaee |
spellingShingle |
M Ranjbar JJ Sendi A Zibaee Proteolytic activity in the midgut of Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae), Pomegranate carob moth Invertebrate Survival Journal Ectomyelois ceratoniae midgut protease characterization |
author_facet |
M Ranjbar JJ Sendi A Zibaee |
author_sort |
M Ranjbar |
title |
Proteolytic activity in the midgut of Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae), Pomegranate carob moth |
title_short |
Proteolytic activity in the midgut of Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae), Pomegranate carob moth |
title_full |
Proteolytic activity in the midgut of Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae), Pomegranate carob moth |
title_fullStr |
Proteolytic activity in the midgut of Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae), Pomegranate carob moth |
title_full_unstemmed |
Proteolytic activity in the midgut of Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae), Pomegranate carob moth |
title_sort |
proteolytic activity in the midgut of ectomyelois ceratoniae zeller (lepidoptera: pyralidae), pomegranate carob moth |
publisher |
University of Modena and Reggio Emilia |
series |
Invertebrate Survival Journal |
issn |
1824-307X |
publishDate |
2011-07-01 |
description |
In this study, the proteolytic activity in the midgut of Ectomyelois ceratoniae as the major pest of pomegranate was investigated to find nature of specific proteases and their properties for adopting possible pest management procedure. It was found that fourth and fifth instar larvae had the highest proteolytic activity as well as specific proteinases including, elastase, trypsin-like, chymotrypsin-like and two exopeptidases. The optimal pH of general protease was 10 and 9 for azocasein, casein and hemoglobin as substrate. The optimal temperature of the total proteolytic activity in the midgut of E. ceratoniae was found 30 and 35 °C by using azocasein and casein as substrates, respectively. In case of hemoglobin, the enzyme showed the highest enzymatic activity at temperatures from 15 to 35 °C. There was no enhancement in the proteolytic activity by using different cations but SDS, citric acid and mercaptoetahnol significantly decreased the proteolytic activity in the midgut of E. ceratoniae. Using specific proteolytic inhibitors including PMSF, TLCK, TPCK, E-64, DTT and phenanthroline revealed presence of serine proteases as the major proteases in the midgut of E. ceratoniae. |
topic |
Ectomyelois ceratoniae midgut protease characterization |
url |
http://www.isj.unimo.it/articoli/ISJ238.pdf |
work_keys_str_mv |
AT mranjbar proteolyticactivityinthemidgutofectomyeloisceratoniaezellerlepidopterapyralidaepomegranatecarobmoth AT jjsendi proteolyticactivityinthemidgutofectomyeloisceratoniaezellerlepidopterapyralidaepomegranatecarobmoth AT azibaee proteolyticactivityinthemidgutofectomyeloisceratoniaezellerlepidopterapyralidaepomegranatecarobmoth |
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