Baicalin Depresses the Sympathoexcitatory Reflex Induced by Myocardial Ischemia via the Dorsal Root Ganglia
Myocardial ischemia (MI) is one of the major causes of death in cardiac diseases. Purinergic signaling is involved in bidirectional neuronal-glial communication in the primary sensory ganglia. The sensory neuritis of cardiac afferent neurons in cervical dorsal root ganglion (cDRG) interacts with car...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2018.00928/full |
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Article |
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DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Lifang Zou Lifang Zou Xinyao Han Shuangmei Liu Shuangmei Liu Yingxin Gong Bing Wu Bing Wu Zhihua Yi Zhihua Yi Hui Liu Hui Liu Shanhong Zhao Shanhong Zhao Tianyu Jia Tianyu Jia Lin Li Lin Li Huilong Yuan Huilong Yuan Liran Shi Liran Shi Chunping Zhang Chunping Zhang Yun Gao Yun Gao Guilin Li Guilin Li Hong Xu Hong Xu Shangdong Liang Shangdong Liang |
| spellingShingle |
Lifang Zou Lifang Zou Xinyao Han Shuangmei Liu Shuangmei Liu Yingxin Gong Bing Wu Bing Wu Zhihua Yi Zhihua Yi Hui Liu Hui Liu Shanhong Zhao Shanhong Zhao Tianyu Jia Tianyu Jia Lin Li Lin Li Huilong Yuan Huilong Yuan Liran Shi Liran Shi Chunping Zhang Chunping Zhang Yun Gao Yun Gao Guilin Li Guilin Li Hong Xu Hong Xu Shangdong Liang Shangdong Liang Baicalin Depresses the Sympathoexcitatory Reflex Induced by Myocardial Ischemia via the Dorsal Root Ganglia Frontiers in Physiology P2Y12 receptor dorsal root ganglia satellite glial cells myocardial ischemia sympathoexcitatory reflex |
| author_facet |
Lifang Zou Lifang Zou Xinyao Han Shuangmei Liu Shuangmei Liu Yingxin Gong Bing Wu Bing Wu Zhihua Yi Zhihua Yi Hui Liu Hui Liu Shanhong Zhao Shanhong Zhao Tianyu Jia Tianyu Jia Lin Li Lin Li Huilong Yuan Huilong Yuan Liran Shi Liran Shi Chunping Zhang Chunping Zhang Yun Gao Yun Gao Guilin Li Guilin Li Hong Xu Hong Xu Shangdong Liang Shangdong Liang |
| author_sort |
Lifang Zou |
| title |
Baicalin Depresses the Sympathoexcitatory Reflex Induced by Myocardial Ischemia via the Dorsal Root Ganglia |
| title_short |
Baicalin Depresses the Sympathoexcitatory Reflex Induced by Myocardial Ischemia via the Dorsal Root Ganglia |
| title_full |
Baicalin Depresses the Sympathoexcitatory Reflex Induced by Myocardial Ischemia via the Dorsal Root Ganglia |
| title_fullStr |
Baicalin Depresses the Sympathoexcitatory Reflex Induced by Myocardial Ischemia via the Dorsal Root Ganglia |
| title_full_unstemmed |
Baicalin Depresses the Sympathoexcitatory Reflex Induced by Myocardial Ischemia via the Dorsal Root Ganglia |
| title_sort |
baicalin depresses the sympathoexcitatory reflex induced by myocardial ischemia via the dorsal root ganglia |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Physiology |
| issn |
1664-042X |
| publishDate |
2018-07-01 |
| description |
Myocardial ischemia (MI) is one of the major causes of death in cardiac diseases. Purinergic signaling is involved in bidirectional neuronal-glial communication in the primary sensory ganglia. The sensory neuritis of cardiac afferent neurons in cervical dorsal root ganglion (cDRG) interacts with cardiac sympathetic efferent postganglionic neurons, forming feedback loops. The P2Y12 receptor is expressed in satellite glial cells (SGCs) of DRG. Baicalin is a major active ingredient extracted from natural herbal medicines, which has anti-inflammatory and strong anti-oxidation properties. In this study we investigated the effect of baicalin on P2Y12 receptor in the cervical DRG SGC-mediated sympathoexcitatory reflex, which is increased during MI. The results showed that the expression of P2Y12 receptor mRNA and protein in DRG, and the co-localization values of P2Y12 receptor and glial fibrillary acidic protein (GFAP) in cDRG SGCs were increased after MI. The activated SGCs increased IL-1β protein expression and elevated Akt phosphorylation in cDRG. Baicalin treatment inhibited the upregulation of the P2Y12 receptor, GFAP protein and Akt phosphorylation in cDRG neurons/SGCs. The stellate ganglia (SG) affect cardiac sympathetic activity. Baicalin treatment also decreased the upregulation of the P2Y12 receptor, GFAP protein in the SG. The P2Y12 agonist, 2Me-SADP, increased [Ca2+]i in HEK293 cells transfected with the P2Y12 receptor plasmid and SGCs in cDRG. These results indicate that application of baicalin alleviates pathologic sympathetic activity induced by MI via inhibition of afferents in the cDRG. |
| topic |
P2Y12 receptor dorsal root ganglia satellite glial cells myocardial ischemia sympathoexcitatory reflex |
| url |
https://www.frontiersin.org/article/10.3389/fphys.2018.00928/full |
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doaj-2e4d1d751f764f6a89c39485c1094bb32020-11-24T23:10:18ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-07-01910.3389/fphys.2018.00928327541Baicalin Depresses the Sympathoexcitatory Reflex Induced by Myocardial Ischemia via the Dorsal Root GangliaLifang Zou0Lifang Zou1Xinyao Han2Shuangmei Liu3Shuangmei Liu4Yingxin Gong5Bing Wu6Bing Wu7Zhihua Yi8Zhihua Yi9Hui Liu10Hui Liu11Shanhong Zhao12Shanhong Zhao13Tianyu Jia14Tianyu Jia15Lin Li16Lin Li17Huilong Yuan18Huilong Yuan19Liran Shi20Liran Shi21Chunping Zhang22Chunping Zhang23Yun Gao24Yun Gao25Guilin Li26Guilin Li27Hong Xu28Hong Xu29Shangdong Liang30Shangdong Liang31Department of Physiology, Medical School of Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaFirst Clinical Department, Medical School of Nanchang University, Nanchang, ChinaDepartment of Physiology, Medical School of Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaFirst Clinical Department, Medical School of Nanchang University, Nanchang, ChinaDepartment of Physiology, Medical School of Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaDepartment of Physiology, Medical School of Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaDepartment of Physiology, Medical School of Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaDepartment of Physiology, Medical School of Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaDepartment of Physiology, Medical School of Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaDepartment of Physiology, Medical School of Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaDepartment of Physiology, Medical School of Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaDepartment of Physiology, Medical School of Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaDepartment of Cell Biology, Medical School of Nanchang University, Nanchang, ChinaDepartment of Physiology, Medical School of Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaDepartment of Physiology, Medical School of Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaDepartment of Physiology, Medical School of Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaDepartment of Physiology, Medical School of Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaMyocardial ischemia (MI) is one of the major causes of death in cardiac diseases. Purinergic signaling is involved in bidirectional neuronal-glial communication in the primary sensory ganglia. The sensory neuritis of cardiac afferent neurons in cervical dorsal root ganglion (cDRG) interacts with cardiac sympathetic efferent postganglionic neurons, forming feedback loops. The P2Y12 receptor is expressed in satellite glial cells (SGCs) of DRG. Baicalin is a major active ingredient extracted from natural herbal medicines, which has anti-inflammatory and strong anti-oxidation properties. In this study we investigated the effect of baicalin on P2Y12 receptor in the cervical DRG SGC-mediated sympathoexcitatory reflex, which is increased during MI. The results showed that the expression of P2Y12 receptor mRNA and protein in DRG, and the co-localization values of P2Y12 receptor and glial fibrillary acidic protein (GFAP) in cDRG SGCs were increased after MI. The activated SGCs increased IL-1β protein expression and elevated Akt phosphorylation in cDRG. Baicalin treatment inhibited the upregulation of the P2Y12 receptor, GFAP protein and Akt phosphorylation in cDRG neurons/SGCs. The stellate ganglia (SG) affect cardiac sympathetic activity. Baicalin treatment also decreased the upregulation of the P2Y12 receptor, GFAP protein in the SG. The P2Y12 agonist, 2Me-SADP, increased [Ca2+]i in HEK293 cells transfected with the P2Y12 receptor plasmid and SGCs in cDRG. These results indicate that application of baicalin alleviates pathologic sympathetic activity induced by MI via inhibition of afferents in the cDRG.https://www.frontiersin.org/article/10.3389/fphys.2018.00928/fullP2Y12 receptordorsal root gangliasatellite glial cellsmyocardial ischemiasympathoexcitatory reflex |