Co-Acquired Nanovirus and Geminivirus Exhibit a Contrasted Localization within Their Common Aphid Vector
Single-stranded DNA (ssDNA) plant viruses belong to the families <i>Geminiviridae</i> and <i>Nanoviridae</i>. They are transmitted by Hemipteran insects in a circulative, mostly non-propagative, manner. While geminiviruses are transmitted by leafhoppers, treehoppers, whitefli...
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doaj-2e5511b53ee74385a06b5030466eb8562020-11-25T03:10:36ZengMDPI AGViruses1999-49152020-03-0112329910.3390/v12030299v12030299Co-Acquired Nanovirus and Geminivirus Exhibit a Contrasted Localization within Their Common Aphid VectorJérémy Di Mattia0Faustine Ryckebusch1Marie-Stéphanie Vernerey2Elodie Pirolles3Nicolas Sauvion4Michel Peterschmitt5Jean-Louis Zeddam6Stéphane Blanc7UMR BGPI, Univ. Montpellier, INRAE, CIRAD, Montpellier SupAgro, 34398 Montpellier, FranceUMR BGPI, Univ. Montpellier, INRAE, CIRAD, Montpellier SupAgro, 34398 Montpellier, FranceUMR BGPI, Univ. Montpellier, INRAE, CIRAD, Montpellier SupAgro, 34398 Montpellier, FranceUMR BGPI, Univ. Montpellier, INRAE, CIRAD, Montpellier SupAgro, 34398 Montpellier, FranceUMR BGPI, Univ. Montpellier, INRAE, CIRAD, Montpellier SupAgro, 34398 Montpellier, FranceUMR BGPI, Univ. Montpellier, INRAE, CIRAD, Montpellier SupAgro, 34398 Montpellier, FranceUMR BGPI, Univ. Montpellier, INRAE, CIRAD, Montpellier SupAgro, 34398 Montpellier, FranceUMR BGPI, Univ. Montpellier, INRAE, CIRAD, Montpellier SupAgro, 34398 Montpellier, FranceSingle-stranded DNA (ssDNA) plant viruses belong to the families <i>Geminiviridae</i> and <i>Nanoviridae</i>. They are transmitted by Hemipteran insects in a circulative, mostly non-propagative, manner. While geminiviruses are transmitted by leafhoppers, treehoppers, whiteflies and aphids, nanoviruses are transmitted exclusively by aphids. Circulative transmission involves complex virus−vector interactions in which epithelial cells have to be crossed and defense mechanisms counteracted. Vector taxa are considered a relevant taxonomic criterion for virus classification, indicating that viruses can evolve specific interactions with their vectors. Thus, we predicted that, although nanoviruses and geminiviruses represent related viral families, they have evolved distinct interactions with their vector. This prediction is also supported by the non-structural Nuclear Shuttle Protein (NSP) that is involved in vector transmission in nanoviruses but has no similar function in geminiviruses. Thanks to the recent discovery of aphid-transmitted geminiviruses, this prediction could be tested for the geminivirus alfalfa leaf curl virus (ALCV) and the nanovirus faba bean necrotic stunt virus (FBNSV) in their common vector, <i>Aphis craccivora</i>. Estimations of viral load in midgut and head of aphids, precise localization of viral DNA in cells of insect vectors and host plants, and virus transmission tests revealed that the pathway of the two viruses across the body of their common vector differs both quantitatively and qualitatively.https://www.mdpi.com/1999-4915/12/3/299fluorescent in situ hybridizationqpcrleaf discshemipterananovirusgeminivirusvector transmission |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jérémy Di Mattia Faustine Ryckebusch Marie-Stéphanie Vernerey Elodie Pirolles Nicolas Sauvion Michel Peterschmitt Jean-Louis Zeddam Stéphane Blanc |
spellingShingle |
Jérémy Di Mattia Faustine Ryckebusch Marie-Stéphanie Vernerey Elodie Pirolles Nicolas Sauvion Michel Peterschmitt Jean-Louis Zeddam Stéphane Blanc Co-Acquired Nanovirus and Geminivirus Exhibit a Contrasted Localization within Their Common Aphid Vector Viruses fluorescent in situ hybridization qpcr leaf discs hemiptera nanovirus geminivirus vector transmission |
author_facet |
Jérémy Di Mattia Faustine Ryckebusch Marie-Stéphanie Vernerey Elodie Pirolles Nicolas Sauvion Michel Peterschmitt Jean-Louis Zeddam Stéphane Blanc |
author_sort |
Jérémy Di Mattia |
title |
Co-Acquired Nanovirus and Geminivirus Exhibit a Contrasted Localization within Their Common Aphid Vector |
title_short |
Co-Acquired Nanovirus and Geminivirus Exhibit a Contrasted Localization within Their Common Aphid Vector |
title_full |
Co-Acquired Nanovirus and Geminivirus Exhibit a Contrasted Localization within Their Common Aphid Vector |
title_fullStr |
Co-Acquired Nanovirus and Geminivirus Exhibit a Contrasted Localization within Their Common Aphid Vector |
title_full_unstemmed |
Co-Acquired Nanovirus and Geminivirus Exhibit a Contrasted Localization within Their Common Aphid Vector |
title_sort |
co-acquired nanovirus and geminivirus exhibit a contrasted localization within their common aphid vector |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2020-03-01 |
description |
Single-stranded DNA (ssDNA) plant viruses belong to the families <i>Geminiviridae</i> and <i>Nanoviridae</i>. They are transmitted by Hemipteran insects in a circulative, mostly non-propagative, manner. While geminiviruses are transmitted by leafhoppers, treehoppers, whiteflies and aphids, nanoviruses are transmitted exclusively by aphids. Circulative transmission involves complex virus−vector interactions in which epithelial cells have to be crossed and defense mechanisms counteracted. Vector taxa are considered a relevant taxonomic criterion for virus classification, indicating that viruses can evolve specific interactions with their vectors. Thus, we predicted that, although nanoviruses and geminiviruses represent related viral families, they have evolved distinct interactions with their vector. This prediction is also supported by the non-structural Nuclear Shuttle Protein (NSP) that is involved in vector transmission in nanoviruses but has no similar function in geminiviruses. Thanks to the recent discovery of aphid-transmitted geminiviruses, this prediction could be tested for the geminivirus alfalfa leaf curl virus (ALCV) and the nanovirus faba bean necrotic stunt virus (FBNSV) in their common vector, <i>Aphis craccivora</i>. Estimations of viral load in midgut and head of aphids, precise localization of viral DNA in cells of insect vectors and host plants, and virus transmission tests revealed that the pathway of the two viruses across the body of their common vector differs both quantitatively and qualitatively. |
topic |
fluorescent in situ hybridization qpcr leaf discs hemiptera nanovirus geminivirus vector transmission |
url |
https://www.mdpi.com/1999-4915/12/3/299 |
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