The effect of titanium dioxide nanoparticles on mice midbrain substantia nigra
Objective(s): Widely used Titanium dioxide nanoparticles (TiO2) enter into the body and cause various organ damages. Therefore, we aimed to study the effect of TiO2 on the substantia nigra of midbrain. Materials and Methods: 40 male BALB/c mice were randomly divided into five groups: three groups re...
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doaj-2e6ca235249a4cc6a042731c670343172020-11-25T01:38:53ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences 2008-38662008-38742019-07-0122774575110.22038/ijbms.2019.33611.801813162The effect of titanium dioxide nanoparticles on mice midbrain substantia nigraZahra Heidari0Abbas Mohammadipour1Parisa Haeri2Alireza Ebrahimzadeh-bideskan3Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran|Microanatomy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran|Microanatomy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranObjective(s): Widely used Titanium dioxide nanoparticles (TiO2) enter into the body and cause various organ damages. Therefore, we aimed to study the effect of TiO2 on the substantia nigra of midbrain. Materials and Methods: 40 male BALB/c mice were randomly divided into five groups: three groups received TiO2 at doses of 10, 25, and 50 mg/kg, the fourth group received normal saline for 45 days by gavage, and control group (without intervention). Then, Motor tests including pole and hanging tests were done to investigate motor disorders. The animal brain was removed for histological purposes. Accordingly, immunohistochemistry was performed to detect tyrosine hydroxylase positive cells, and then toluidine blue staining was done to identify dark neurons in the substantia nigra. Eventually, the total number of these neurons were counted using stereological methods in different groups.Results: The results showed that the time recorded for mice to turn completely downward on the pole in the TiO2-50 group increased and also the time recorded for animals to hang on the wire in the hanging test significantly decreased (Phttp://ijbms.mums.ac.ir/article_13162_7a514878e78959136bce619c6aeaa9ad.pdfDark neuronsMiceSubstantia nigraTitanium dioxide nanoparticlesTyrosine hydroxylase neurons |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zahra Heidari Abbas Mohammadipour Parisa Haeri Alireza Ebrahimzadeh-bideskan |
spellingShingle |
Zahra Heidari Abbas Mohammadipour Parisa Haeri Alireza Ebrahimzadeh-bideskan The effect of titanium dioxide nanoparticles on mice midbrain substantia nigra Iranian Journal of Basic Medical Sciences Dark neurons Mice Substantia nigra Titanium dioxide nanoparticles Tyrosine hydroxylase neurons |
author_facet |
Zahra Heidari Abbas Mohammadipour Parisa Haeri Alireza Ebrahimzadeh-bideskan |
author_sort |
Zahra Heidari |
title |
The effect of titanium dioxide nanoparticles on mice midbrain substantia nigra |
title_short |
The effect of titanium dioxide nanoparticles on mice midbrain substantia nigra |
title_full |
The effect of titanium dioxide nanoparticles on mice midbrain substantia nigra |
title_fullStr |
The effect of titanium dioxide nanoparticles on mice midbrain substantia nigra |
title_full_unstemmed |
The effect of titanium dioxide nanoparticles on mice midbrain substantia nigra |
title_sort |
effect of titanium dioxide nanoparticles on mice midbrain substantia nigra |
publisher |
Mashhad University of Medical Sciences |
series |
Iranian Journal of Basic Medical Sciences |
issn |
2008-3866 2008-3874 |
publishDate |
2019-07-01 |
description |
Objective(s): Widely used Titanium dioxide nanoparticles (TiO2) enter into the body and cause various organ damages. Therefore, we aimed to study the effect of TiO2 on the substantia nigra of midbrain. Materials and Methods: 40 male BALB/c mice were randomly divided into five groups: three groups received TiO2 at doses of 10, 25, and 50 mg/kg, the fourth group received normal saline for 45 days by gavage, and control group (without intervention). Then, Motor tests including pole and hanging tests were done to investigate motor disorders. The animal brain was removed for histological purposes. Accordingly, immunohistochemistry was performed to detect tyrosine hydroxylase positive cells, and then toluidine blue staining was done to identify dark neurons in the substantia nigra. Eventually, the total number of these neurons were counted using stereological methods in different groups.Results: The results showed that the time recorded for mice to turn completely downward on the pole in the TiO2-50 group increased and also the time recorded for animals to hang on the wire in the hanging test significantly decreased (P |
topic |
Dark neurons Mice Substantia nigra Titanium dioxide nanoparticles Tyrosine hydroxylase neurons |
url |
http://ijbms.mums.ac.ir/article_13162_7a514878e78959136bce619c6aeaa9ad.pdf |
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