Reactive oxygen species mediate TNF-α-induced inflammatory response in bone marrow mesenchymal cells
<em><strong>Objective(s):</strong></em> It is generally believed that the inflammatory response in bone marrow mesenchymal stem cells (BMSCs) transplantation leads to poor survival and unsatisfactory effects, and is mainly mediated by cytokines, including interleukin-1β (IL-1...
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doaj-2e8063eab82841abb196aa81ff9fac1a2020-11-25T01:44:28ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences 2008-38662008-38742019-11-0122111296130110.22038/ijbms.2019.37893.900613885Reactive oxygen species mediate TNF-α-induced inflammatory response in bone marrow mesenchymal cellsLiuzhong Wu0Yaping Pan1Department of Periodontics, School of Stomatology, China Medical University, Liaoning Province, China|Department of Periodontics, Shenyang Stomatological Hospital, Shenyang, Liaoning Province, ChinaDepartment of Periodontics, School of Stomatology, China Medical University, Liaoning Province, China<em><strong>Objective(s):</strong></em> It is generally believed that the inflammatory response in bone marrow mesenchymal stem cells (BMSCs) transplantation leads to poor survival and unsatisfactory effects, and is mainly mediated by cytokines, including interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α). In this study, we explored the mechanisms underlying the TNF-α-induced inflammatory response in BMSCs.<br /> <em><strong>Materials and Methods:</strong></em> We treated BMSCs with TNF-α (1 and 10 ng/ml) for 5 days. The expression levels of key inflammatory mediators were evaluated by Real-time PCR. Intracellular ROS level was measured by using a 2, 7-dichlorofluorescein diacetate (DCF-DA).<br /><em><strong>Results:</strong></em> We found that TNF-α treatment dramatically increased the expression levels of some key inflammatory mediators, including IL-6, IL-1β, IFN-γ and transforming growth factor β (TGF-β). Moreover, TNF-α induced intracellular oxidative stress by elevating intracellular reactive oxygen species (ROS) level, which is due to the increase of lipid peroxidation, the reduction of antioxidant Glutathione (GSH) levels and the inhibition of many antioxidant enzyme activities in BMSCs. Interestingly, 5 µM curcumin, a ROS scavenger, dramatically lowered the TNF-α-induced inflammatory response in BMSCs. In addition, TNF-α induced the activation of extracellular-signal-regulated kinases 1/2 (ERK1/2), c-Jun N-terminal kinases (JNK), p38 and their down-stream transcription factors nuclear factor kappa B (NF-κB) pathway.<br /><em><strong>Conclusion:</strong></em> ROS mediated the TNF-α-induced inflammatory response via MAPK and NF-κB pathway, and may provide a novel strategy to prevent the inflammatory-dependent impairments in BMSCs.http://ijbms.mums.ac.ir/article_13885_3503437c7566d04c5fd85c824589909b.pdfbmscsinflammation responsemapknf-κboxidative stresstnf-α |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liuzhong Wu Yaping Pan |
spellingShingle |
Liuzhong Wu Yaping Pan Reactive oxygen species mediate TNF-α-induced inflammatory response in bone marrow mesenchymal cells Iranian Journal of Basic Medical Sciences bmscs inflammation response mapk nf-κb oxidative stress tnf-α |
author_facet |
Liuzhong Wu Yaping Pan |
author_sort |
Liuzhong Wu |
title |
Reactive oxygen species mediate TNF-α-induced inflammatory response in bone marrow mesenchymal cells |
title_short |
Reactive oxygen species mediate TNF-α-induced inflammatory response in bone marrow mesenchymal cells |
title_full |
Reactive oxygen species mediate TNF-α-induced inflammatory response in bone marrow mesenchymal cells |
title_fullStr |
Reactive oxygen species mediate TNF-α-induced inflammatory response in bone marrow mesenchymal cells |
title_full_unstemmed |
Reactive oxygen species mediate TNF-α-induced inflammatory response in bone marrow mesenchymal cells |
title_sort |
reactive oxygen species mediate tnf-α-induced inflammatory response in bone marrow mesenchymal cells |
publisher |
Mashhad University of Medical Sciences |
series |
Iranian Journal of Basic Medical Sciences |
issn |
2008-3866 2008-3874 |
publishDate |
2019-11-01 |
description |
<em><strong>Objective(s):</strong></em> It is generally believed that the inflammatory response in bone marrow mesenchymal stem cells (BMSCs) transplantation leads to poor survival and unsatisfactory effects, and is mainly mediated by cytokines, including interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α). In this study, we explored the mechanisms underlying the TNF-α-induced inflammatory response in BMSCs.<br /> <em><strong>Materials and Methods:</strong></em> We treated BMSCs with TNF-α (1 and 10 ng/ml) for 5 days. The expression levels of key inflammatory mediators were evaluated by Real-time PCR. Intracellular ROS level was measured by using a 2, 7-dichlorofluorescein diacetate (DCF-DA).<br /><em><strong>Results:</strong></em> We found that TNF-α treatment dramatically increased the expression levels of some key inflammatory mediators, including IL-6, IL-1β, IFN-γ and transforming growth factor β (TGF-β). Moreover, TNF-α induced intracellular oxidative stress by elevating intracellular reactive oxygen species (ROS) level, which is due to the increase of lipid peroxidation, the reduction of antioxidant Glutathione (GSH) levels and the inhibition of many antioxidant enzyme activities in BMSCs. Interestingly, 5 µM curcumin, a ROS scavenger, dramatically lowered the TNF-α-induced inflammatory response in BMSCs. In addition, TNF-α induced the activation of extracellular-signal-regulated kinases 1/2 (ERK1/2), c-Jun N-terminal kinases (JNK), p38 and their down-stream transcription factors nuclear factor kappa B (NF-κB) pathway.<br /><em><strong>Conclusion:</strong></em> ROS mediated the TNF-α-induced inflammatory response via MAPK and NF-κB pathway, and may provide a novel strategy to prevent the inflammatory-dependent impairments in BMSCs. |
topic |
bmscs inflammation response mapk nf-κb oxidative stress tnf-α |
url |
http://ijbms.mums.ac.ir/article_13885_3503437c7566d04c5fd85c824589909b.pdf |
work_keys_str_mv |
AT liuzhongwu reactiveoxygenspeciesmediatetnfainducedinflammatoryresponseinbonemarrowmesenchymalcells AT yapingpan reactiveoxygenspeciesmediatetnfainducedinflammatoryresponseinbonemarrowmesenchymalcells |
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1725028561834737664 |