Trichuris trichiura isolated from Macaca sylvanus: morphological, biometrical, and molecular study
Abstract Background Recent studies have reported the existence of a Trichuris species complex parasitizing primate. Nevertheless, the genetic and evolutionary relationship between Trichuris spp. parasitizing humans and Non-Human Primates (NHP) is poorly understood. The hypothesised existence of diff...
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doaj-2ea222c022504d3ebd017f037efe4eec2020-11-25T04:12:29ZengBMCBMC Veterinary Research1746-61482020-11-0116111910.1186/s12917-020-02661-4Trichuris trichiura isolated from Macaca sylvanus: morphological, biometrical, and molecular studyJulia Rivero0Ángela María García-Sánchez1Antonio Zurita2Cristina Cutillas3Rocío Callejón4Department of Microbiology and Parasitology, Faculty of Pharmacy, University of SevilleDepartment of Microbiology and Parasitology, Faculty of Pharmacy, University of SevilleDepartment of Microbiology and Parasitology, Faculty of Pharmacy, University of SevilleDepartment of Microbiology and Parasitology, Faculty of Pharmacy, University of SevilleDepartment of Microbiology and Parasitology, Faculty of Pharmacy, University of SevilleAbstract Background Recent studies have reported the existence of a Trichuris species complex parasitizing primate. Nevertheless, the genetic and evolutionary relationship between Trichuris spp. parasitizing humans and Non-Human Primates (NHP) is poorly understood. The hypothesised existence of different species of Trichuris in primates opens the possibility to evaluate these primates as reservoir hosts of human trichuriasis and other putative new species of whipworms. Results In this paper, we carried out a morphological, biometrical and molecular study of Trichuris population parasitizing Macaca sylvanus from Spain based on traditional morpho-biometrical methods, PCA analysis and ribosomal (ITS2) and mitochondrial (cox1 and cob) DNA sequencing. Morphological results revealed that Trichuris sp. from M. sylvanus is Trichuris trichiura. Ribosomal datasets revealed that phylogenetic relationships of populations of Trichuris sp. from M. sylvanus were unresolved. The phylogeny inferred on mitochondrial datasets (partitioned and concatenated) revealed similar topologies; Thus, phylogenetic trees supported the existence of clear molecular differentiation between individuals of Trichuris sp. from M. sylvanus appearing in two different subclades. Conclusions Based on morphological parameters, biometrical measurements, and molecular sequence analysis, we conclude that the whipworms isolated from M. sylvanus were T. trichiura. Further, the evolutionary relationship showed that these worms belonged to two genotypes within the T. trichiura lineage. Since T. trichiura is of public health importance, it is important to carry out further studies to improve the understanding of its hosts range, evolution and phylogeography.http://link.springer.com/article/10.1186/s12917-020-02661-4Barbary macaqueMacaca sylvanusMitochondrial DNATrichuris trichiuraZoonosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Julia Rivero Ángela María García-Sánchez Antonio Zurita Cristina Cutillas Rocío Callejón |
spellingShingle |
Julia Rivero Ángela María García-Sánchez Antonio Zurita Cristina Cutillas Rocío Callejón Trichuris trichiura isolated from Macaca sylvanus: morphological, biometrical, and molecular study BMC Veterinary Research Barbary macaque Macaca sylvanus Mitochondrial DNA Trichuris trichiura Zoonosis |
author_facet |
Julia Rivero Ángela María García-Sánchez Antonio Zurita Cristina Cutillas Rocío Callejón |
author_sort |
Julia Rivero |
title |
Trichuris trichiura isolated from Macaca sylvanus: morphological, biometrical, and molecular study |
title_short |
Trichuris trichiura isolated from Macaca sylvanus: morphological, biometrical, and molecular study |
title_full |
Trichuris trichiura isolated from Macaca sylvanus: morphological, biometrical, and molecular study |
title_fullStr |
Trichuris trichiura isolated from Macaca sylvanus: morphological, biometrical, and molecular study |
title_full_unstemmed |
Trichuris trichiura isolated from Macaca sylvanus: morphological, biometrical, and molecular study |
title_sort |
trichuris trichiura isolated from macaca sylvanus: morphological, biometrical, and molecular study |
publisher |
BMC |
series |
BMC Veterinary Research |
issn |
1746-6148 |
publishDate |
2020-11-01 |
description |
Abstract Background Recent studies have reported the existence of a Trichuris species complex parasitizing primate. Nevertheless, the genetic and evolutionary relationship between Trichuris spp. parasitizing humans and Non-Human Primates (NHP) is poorly understood. The hypothesised existence of different species of Trichuris in primates opens the possibility to evaluate these primates as reservoir hosts of human trichuriasis and other putative new species of whipworms. Results In this paper, we carried out a morphological, biometrical and molecular study of Trichuris population parasitizing Macaca sylvanus from Spain based on traditional morpho-biometrical methods, PCA analysis and ribosomal (ITS2) and mitochondrial (cox1 and cob) DNA sequencing. Morphological results revealed that Trichuris sp. from M. sylvanus is Trichuris trichiura. Ribosomal datasets revealed that phylogenetic relationships of populations of Trichuris sp. from M. sylvanus were unresolved. The phylogeny inferred on mitochondrial datasets (partitioned and concatenated) revealed similar topologies; Thus, phylogenetic trees supported the existence of clear molecular differentiation between individuals of Trichuris sp. from M. sylvanus appearing in two different subclades. Conclusions Based on morphological parameters, biometrical measurements, and molecular sequence analysis, we conclude that the whipworms isolated from M. sylvanus were T. trichiura. Further, the evolutionary relationship showed that these worms belonged to two genotypes within the T. trichiura lineage. Since T. trichiura is of public health importance, it is important to carry out further studies to improve the understanding of its hosts range, evolution and phylogeography. |
topic |
Barbary macaque Macaca sylvanus Mitochondrial DNA Trichuris trichiura Zoonosis |
url |
http://link.springer.com/article/10.1186/s12917-020-02661-4 |
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