Testing the Applicability of MALDI-TOF MS as an Alternative Stock Identification Method in a Cryptic Species Complex
Knowledge of intraspecific variability of a certain species is essential for their long-term survival and for the development of conservation plans. Nowadays, molecular/genetic methods are the most frequently used for this purpose. Although, the Matrix Assisted Laser Desorption Ionization Time of Fl...
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doaj-2eace5b85c294fe4b9beba42ffa8f96f2020-11-25T02:36:55ZengMDPI AGMolecules1420-30492020-07-01253214321410.3390/molecules25143214Testing the Applicability of MALDI-TOF MS as an Alternative Stock Identification Method in a Cryptic Species ComplexGabor Maasz0Zita Zrínyi1Istvan Fodor2Nóra Boross3Zoltán Vitál4Dóra Ildikó Kánainé Sipos5Balázs Kovács6Szilvia Melegh7Péter Takács8Balaton Limnological Institute, Centre for Ecological Research, Klebelsberg Kuno street 3, 8237 Tihany, HungaryBalaton Limnological Institute, Centre for Ecological Research, Klebelsberg Kuno street 3, 8237 Tihany, HungaryBalaton Limnological Institute, Centre for Ecological Research, Klebelsberg Kuno street 3, 8237 Tihany, HungaryBalaton Limnological Institute, Centre for Ecological Research, Klebelsberg Kuno street 3, 8237 Tihany, HungaryBalaton Limnological Institute, Centre for Ecological Research, Klebelsberg Kuno street 3, 8237 Tihany, HungaryDepartment of Aquaculture, Szent István University, Páter Károly str. 1, 2100 Gödöllő, HungaryDepartment of Aquaculture, Szent István University, Páter Károly str. 1, 2100 Gödöllő, HungaryDepartment of Medical Microbiology and Immunology, Medical School, University of Pécs, Szigeti str. 12, 7624 Pécs, HungaryBalaton Limnological Institute, Centre for Ecological Research, Klebelsberg Kuno street 3, 8237 Tihany, HungaryKnowledge of intraspecific variability of a certain species is essential for their long-term survival and for the development of conservation plans. Nowadays, molecular/genetic methods are the most frequently used for this purpose. Although, the Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) technique has become a promising alternative tool to specify intraspecific variability, there is a lack of information about the limitations of this method, and some methodological issues need to be resolved. Towards this goal, we tested the sensitivity of this method on an intraspecific level, using genetically identified individuals of a cryptic fish species complex collected from five distinct populations. Additionally, some methodologic issues, such as the effect of 1) delayed sample preparation, 2) clove oil anaesthetization, and 3) different tissue types (muscle, and brain) were investigated using the MS analysis results. Our results show that the delayed sample preparation has a fundamental effect on the result of MS analysis, while at the same time the clove oil did not affect the results considerably. Both the brain and muscle samples were usable for cryptic species identification, but in our opinion this method has limited applicability for population-level segregation. The application of MALDI-TOF MS to the exploitable toolkit of phylogenetic and taxonomic researches could be used to broaden conclusions.https://www.mdpi.com/1420-3049/25/14/3214phyloproteomicsMALDI-TOF mass spectrometrygobiofreshwater fishclassificationtaxonomy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gabor Maasz Zita Zrínyi Istvan Fodor Nóra Boross Zoltán Vitál Dóra Ildikó Kánainé Sipos Balázs Kovács Szilvia Melegh Péter Takács |
spellingShingle |
Gabor Maasz Zita Zrínyi Istvan Fodor Nóra Boross Zoltán Vitál Dóra Ildikó Kánainé Sipos Balázs Kovács Szilvia Melegh Péter Takács Testing the Applicability of MALDI-TOF MS as an Alternative Stock Identification Method in a Cryptic Species Complex Molecules phyloproteomics MALDI-TOF mass spectrometry gobio freshwater fish classification taxonomy |
author_facet |
Gabor Maasz Zita Zrínyi Istvan Fodor Nóra Boross Zoltán Vitál Dóra Ildikó Kánainé Sipos Balázs Kovács Szilvia Melegh Péter Takács |
author_sort |
Gabor Maasz |
title |
Testing the Applicability of MALDI-TOF MS as an Alternative Stock Identification Method in a Cryptic Species Complex |
title_short |
Testing the Applicability of MALDI-TOF MS as an Alternative Stock Identification Method in a Cryptic Species Complex |
title_full |
Testing the Applicability of MALDI-TOF MS as an Alternative Stock Identification Method in a Cryptic Species Complex |
title_fullStr |
Testing the Applicability of MALDI-TOF MS as an Alternative Stock Identification Method in a Cryptic Species Complex |
title_full_unstemmed |
Testing the Applicability of MALDI-TOF MS as an Alternative Stock Identification Method in a Cryptic Species Complex |
title_sort |
testing the applicability of maldi-tof ms as an alternative stock identification method in a cryptic species complex |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-07-01 |
description |
Knowledge of intraspecific variability of a certain species is essential for their long-term survival and for the development of conservation plans. Nowadays, molecular/genetic methods are the most frequently used for this purpose. Although, the Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) technique has become a promising alternative tool to specify intraspecific variability, there is a lack of information about the limitations of this method, and some methodological issues need to be resolved. Towards this goal, we tested the sensitivity of this method on an intraspecific level, using genetically identified individuals of a cryptic fish species complex collected from five distinct populations. Additionally, some methodologic issues, such as the effect of 1) delayed sample preparation, 2) clove oil anaesthetization, and 3) different tissue types (muscle, and brain) were investigated using the MS analysis results. Our results show that the delayed sample preparation has a fundamental effect on the result of MS analysis, while at the same time the clove oil did not affect the results considerably. Both the brain and muscle samples were usable for cryptic species identification, but in our opinion this method has limited applicability for population-level segregation. The application of MALDI-TOF MS to the exploitable toolkit of phylogenetic and taxonomic researches could be used to broaden conclusions. |
topic |
phyloproteomics MALDI-TOF mass spectrometry gobio freshwater fish classification taxonomy |
url |
https://www.mdpi.com/1420-3049/25/14/3214 |
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