Real Time Monitoring of a UV Light-Assisted Biofunctionalization Protocol Using a Nanophotonic Biosensor

• Main Authors: Jad Sabek, Luis Torrijos-Morán, Amadeu Griol, Zeneida Díaz Betancor, María-José Bañuls Polo, Ángel Maquieira, Jaime García-Rupérez
• Format: Article
• Language: English
• Published: MDPI AG 2018-12-01
• Series: Biosensors
• Subjects: biofunctionalization; UV light photocatalysis; half antibodies; silicon on insulator; nanophotonic sensor
• Online Access: http://www.mdpi.com/2079-6374/9/1/6

A protocol for the covalent biofunctionalization of silicon-based biosensors using a UV light-induced thiol–ene coupling (TEC) reaction has been developed. This biofunctionalization approach has been used to immobilize half antibodies (hIgG), which have been obtained by means of a tris(2-carboxyethyl)phosphine (TCEP) reduction at the hinge region, to the surface of a vinyl-activated silicon-on-insulator (SOI) nanophotonic sensing chip. The response of the sensing structures within the nanophotonic chip was monitored in real time during the biofunctionalization process, which has allowed us to confirm that the bioconjugation of the thiol-terminated bioreceptors onto the vinyl-activated sensing surface is only initiated upon UV light photocatalysis.