Genome-Wide Identification and Analysis of Arabidopsis Sodium Proton Antiporter (NHX) and Human Sodium Proton Exchanger (NHE) Homologs in Sorghum bicolor
Na+ transporters play an important role during salt stress and development. The present study is aimed at genome-wide identification, in silico analysis of sodium-proton antiporter (NHX) and sodium-proton exchanger (NHE)-type transporters in Sorghum bicolor and their expression patterns under varied...
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doaj-2ec364cdabfc41c2a95766b6d3824beb2020-11-24T23:16:32ZengMDPI AGGenes2073-44252018-05-019523610.3390/genes9050236genes9050236Genome-Wide Identification and Analysis of Arabidopsis Sodium Proton Antiporter (NHX) and Human Sodium Proton Exchanger (NHE) Homologs in Sorghum bicolorP. Hima Kumari0S. Anil Kumar1Katam Ramesh2Palakolanu Sudhakar Reddy3M. Nagaraju4A. Bhanu Prakash5Trushar Shah6Ashley Henderson7Rakesh K. Srivastava8G. Rajasheker9A. Chitikineni10Rajeev K. Varshney11P. Rathnagiri12M. Lakshmi Narasu13P. B. Kavi Kishor14Department of Genetics, Osmania University, Hyderabad 500 007, IndiaDepartment of Genetics, Osmania University, Hyderabad 500 007, IndiaDepartment of Biological Sciences, Florida A&M University, Tallahassee, FL 32307, USACenter of Excellence in Genomics & Systems Biology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad 502 324, IndiaDepartment of Genetics, Osmania University, Hyderabad 500 007, IndiaCenter of Excellence in Genomics & Systems Biology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad 502 324, IndiaIITA-Kenya c/o International Livestock Research Institute (ILRI), PO Box 30709, Nairobi 00100, KenyaDepartment of Biological Sciences, Florida A&M University, Tallahassee, FL 32307, USACenter of Excellence in Genomics & Systems Biology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad 502 324, IndiaDepartment of Genetics, Osmania University, Hyderabad 500 007, IndiaCenter of Excellence in Genomics & Systems Biology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad 502 324, IndiaCenter of Excellence in Genomics & Systems Biology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad 502 324, IndiaGenomix CARL Pvt. Ltd. Rayalapuram Road, Pulivendula, 516 390, Kadapa, Andhra Pradesh, IndiaCentre for Biotechnology, Institute of Science & Technology, JNT University, Hyderabad 500 085, IndiaDepartment of Genetics, Osmania University, Hyderabad 500 007, IndiaNa+ transporters play an important role during salt stress and development. The present study is aimed at genome-wide identification, in silico analysis of sodium-proton antiporter (NHX) and sodium-proton exchanger (NHE)-type transporters in Sorghum bicolor and their expression patterns under varied abiotic stress conditions. In Sorghum, seven NHX and nine NHE homologs were identified. Amiloride (a known inhibitor of Na+/H+ exchanger activity) binding motif was noticed in both types of the transporters. Chromosome 2 was found to be a hotspot region with five sodium transporters. Phylogenetic analysis inferred six ortholog and three paralog groups. To gain an insight into functional divergence of SbNHX/NHE transporters, real-time gene expression was performed under salt, drought, heat, and cold stresses in embryo, root, stem, and leaf tissues. Expression patterns revealed that both SbNHXs and SbNHEs are responsive either to single or multiple abiotic stresses. The predicted protein–protein interaction networks revealed that only SbNHX7 is involved in the calcineurin B-like proteins (CBL)- CBL interacting protein kinases (CIPK) pathway. The study provides insights into the functional divergence of SbNHX/NHE transporter genes with tissue specific expressions in Sorghum under different abiotic stress conditions.http://www.mdpi.com/2073-4425/9/5/236abiotic stressamilorideCBL-CIPK pathwaysodium-proton exchangersodium-proton antiporterSorghum bicolor |
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language |
English |
format |
Article |
sources |
DOAJ |
author |
P. Hima Kumari S. Anil Kumar Katam Ramesh Palakolanu Sudhakar Reddy M. Nagaraju A. Bhanu Prakash Trushar Shah Ashley Henderson Rakesh K. Srivastava G. Rajasheker A. Chitikineni Rajeev K. Varshney P. Rathnagiri M. Lakshmi Narasu P. B. Kavi Kishor |
spellingShingle |
P. Hima Kumari S. Anil Kumar Katam Ramesh Palakolanu Sudhakar Reddy M. Nagaraju A. Bhanu Prakash Trushar Shah Ashley Henderson Rakesh K. Srivastava G. Rajasheker A. Chitikineni Rajeev K. Varshney P. Rathnagiri M. Lakshmi Narasu P. B. Kavi Kishor Genome-Wide Identification and Analysis of Arabidopsis Sodium Proton Antiporter (NHX) and Human Sodium Proton Exchanger (NHE) Homologs in Sorghum bicolor Genes abiotic stress amiloride CBL-CIPK pathway sodium-proton exchanger sodium-proton antiporter Sorghum bicolor |
author_facet |
P. Hima Kumari S. Anil Kumar Katam Ramesh Palakolanu Sudhakar Reddy M. Nagaraju A. Bhanu Prakash Trushar Shah Ashley Henderson Rakesh K. Srivastava G. Rajasheker A. Chitikineni Rajeev K. Varshney P. Rathnagiri M. Lakshmi Narasu P. B. Kavi Kishor |
author_sort |
P. Hima Kumari |
title |
Genome-Wide Identification and Analysis of Arabidopsis Sodium Proton Antiporter (NHX) and Human Sodium Proton Exchanger (NHE) Homologs in Sorghum bicolor |
title_short |
Genome-Wide Identification and Analysis of Arabidopsis Sodium Proton Antiporter (NHX) and Human Sodium Proton Exchanger (NHE) Homologs in Sorghum bicolor |
title_full |
Genome-Wide Identification and Analysis of Arabidopsis Sodium Proton Antiporter (NHX) and Human Sodium Proton Exchanger (NHE) Homologs in Sorghum bicolor |
title_fullStr |
Genome-Wide Identification and Analysis of Arabidopsis Sodium Proton Antiporter (NHX) and Human Sodium Proton Exchanger (NHE) Homologs in Sorghum bicolor |
title_full_unstemmed |
Genome-Wide Identification and Analysis of Arabidopsis Sodium Proton Antiporter (NHX) and Human Sodium Proton Exchanger (NHE) Homologs in Sorghum bicolor |
title_sort |
genome-wide identification and analysis of arabidopsis sodium proton antiporter (nhx) and human sodium proton exchanger (nhe) homologs in sorghum bicolor |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2018-05-01 |
description |
Na+ transporters play an important role during salt stress and development. The present study is aimed at genome-wide identification, in silico analysis of sodium-proton antiporter (NHX) and sodium-proton exchanger (NHE)-type transporters in Sorghum bicolor and their expression patterns under varied abiotic stress conditions. In Sorghum, seven NHX and nine NHE homologs were identified. Amiloride (a known inhibitor of Na+/H+ exchanger activity) binding motif was noticed in both types of the transporters. Chromosome 2 was found to be a hotspot region with five sodium transporters. Phylogenetic analysis inferred six ortholog and three paralog groups. To gain an insight into functional divergence of SbNHX/NHE transporters, real-time gene expression was performed under salt, drought, heat, and cold stresses in embryo, root, stem, and leaf tissues. Expression patterns revealed that both SbNHXs and SbNHEs are responsive either to single or multiple abiotic stresses. The predicted protein–protein interaction networks revealed that only SbNHX7 is involved in the calcineurin B-like proteins (CBL)- CBL interacting protein kinases (CIPK) pathway. The study provides insights into the functional divergence of SbNHX/NHE transporter genes with tissue specific expressions in Sorghum under different abiotic stress conditions. |
topic |
abiotic stress amiloride CBL-CIPK pathway sodium-proton exchanger sodium-proton antiporter Sorghum bicolor |
url |
http://www.mdpi.com/2073-4425/9/5/236 |
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