Beta-adrenergic receptors are critical for weight loss but not for other metabolic adaptations to the consumption of a ketogenic diet in male mice
Objective: We have previously shown that the consumption of a low-carbohydrate ketogenic diet (KD) by mice leads to a distinct physiologic state associated with weight loss, increased metabolic rate, and improved insulin sensitivity [1]. Furthermore, we identified fibroblast growth factor 21 (FGF21)...
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doaj-2ec819be14d54f0ba83cd4ac23e0a9cf2020-11-24T22:05:38ZengElsevierMolecular Metabolism2212-87782017-08-016885486210.1016/j.molmet.2017.05.017Beta-adrenergic receptors are critical for weight loss but not for other metabolic adaptations to the consumption of a ketogenic diet in male miceNicholas Douris0Bhavna N. Desai1ffolliott M. Fisher2Theodore Cisu3Alan J. Fowler4Eleen Zarebidaki5Ngoc Ly T. Nguyen6Donald A. Morgan7Timothy J. Bartness8Kamal Rahmouni9Jeffrey S. Flier10Eleftheria Maratos-Flier11Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USADepartment of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USADepartment of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USADepartment of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USADepartment of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USADepartment of Biology and Center for Obesity Reversal, Georgia State University, Atlanta, GA 30302-4010, USADepartment of Biology and Center for Obesity Reversal, Georgia State University, Atlanta, GA 30302-4010, USADepartment of Pharmacology, University of Iowa, Carver College of Medicine, Iowa City, IA 52242, USADepartment of Biology and Center for Obesity Reversal, Georgia State University, Atlanta, GA 30302-4010, USADepartment of Pharmacology, University of Iowa, Carver College of Medicine, Iowa City, IA 52242, USADepartment of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USADepartment of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USAObjective: We have previously shown that the consumption of a low-carbohydrate ketogenic diet (KD) by mice leads to a distinct physiologic state associated with weight loss, increased metabolic rate, and improved insulin sensitivity [1]. Furthermore, we identified fibroblast growth factor 21 (FGF21) as a necessary mediator of the changes, as mice lacking FGF21 fed KD gain rather than lose weight [2]. FGF21 activates the sympathetic nervous system (SNS) [3], which is a key regulator of metabolic rate. Thus, we considered that the SNS may play a role in mediating the metabolic adaption to ketosis. Methods: To test this hypothesis, we measured the response of mice lacking all three β-adrenergic receptors (β-less mice) to KD feeding. Results: In contrast to wild-type (WT) controls, β-less mice gained weight, increased adipose tissue depots mass, and did not increase energy expenditure when consuming KD. Remarkably, despite weight-gain, β-less mice were insulin sensitive. KD-induced changes in hepatic gene expression of β-less mice were similar to those seen in WT controls eating KD. Expression of FGF21 mRNA rose over 60-fold in both WT and β-less mice fed KD, and corresponding circulating FGF21 levels were 12.5 ng/ml in KD-fed wild type controls and 35.5 ng/ml in KD-fed β-less mice. Conclusions: The response of β-less mice distinguishes at least two distinct categories of physiologic effects in mice consuming KD. In the liver, KD regulates peroxisome proliferator-activated receptor alpha (PPARα)-dependent pathways through an action of FGF21 independent of the SNS and beta-adrenergic receptors. In sharp contrast, induction of interscapular brown adipose tissue (BAT) and increased energy expenditure absolutely require SNS signals involving action on one or more β-adrenergic receptors. In this way, the key metabolic actions of FGF21 in response to KD have diverse effector mechanisms.http://www.sciencedirect.com/science/article/pii/S2212877817303071Ketogenic dietWeight lossSympathetic nervous systemβ-Adrenergic receptors |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nicholas Douris Bhavna N. Desai ffolliott M. Fisher Theodore Cisu Alan J. Fowler Eleen Zarebidaki Ngoc Ly T. Nguyen Donald A. Morgan Timothy J. Bartness Kamal Rahmouni Jeffrey S. Flier Eleftheria Maratos-Flier |
spellingShingle |
Nicholas Douris Bhavna N. Desai ffolliott M. Fisher Theodore Cisu Alan J. Fowler Eleen Zarebidaki Ngoc Ly T. Nguyen Donald A. Morgan Timothy J. Bartness Kamal Rahmouni Jeffrey S. Flier Eleftheria Maratos-Flier Beta-adrenergic receptors are critical for weight loss but not for other metabolic adaptations to the consumption of a ketogenic diet in male mice Molecular Metabolism Ketogenic diet Weight loss Sympathetic nervous system β-Adrenergic receptors |
author_facet |
Nicholas Douris Bhavna N. Desai ffolliott M. Fisher Theodore Cisu Alan J. Fowler Eleen Zarebidaki Ngoc Ly T. Nguyen Donald A. Morgan Timothy J. Bartness Kamal Rahmouni Jeffrey S. Flier Eleftheria Maratos-Flier |
author_sort |
Nicholas Douris |
title |
Beta-adrenergic receptors are critical for weight loss but not for other metabolic adaptations to the consumption of a ketogenic diet in male mice |
title_short |
Beta-adrenergic receptors are critical for weight loss but not for other metabolic adaptations to the consumption of a ketogenic diet in male mice |
title_full |
Beta-adrenergic receptors are critical for weight loss but not for other metabolic adaptations to the consumption of a ketogenic diet in male mice |
title_fullStr |
Beta-adrenergic receptors are critical for weight loss but not for other metabolic adaptations to the consumption of a ketogenic diet in male mice |
title_full_unstemmed |
Beta-adrenergic receptors are critical for weight loss but not for other metabolic adaptations to the consumption of a ketogenic diet in male mice |
title_sort |
beta-adrenergic receptors are critical for weight loss but not for other metabolic adaptations to the consumption of a ketogenic diet in male mice |
publisher |
Elsevier |
series |
Molecular Metabolism |
issn |
2212-8778 |
publishDate |
2017-08-01 |
description |
Objective: We have previously shown that the consumption of a low-carbohydrate ketogenic diet (KD) by mice leads to a distinct physiologic state associated with weight loss, increased metabolic rate, and improved insulin sensitivity [1]. Furthermore, we identified fibroblast growth factor 21 (FGF21) as a necessary mediator of the changes, as mice lacking FGF21 fed KD gain rather than lose weight [2]. FGF21 activates the sympathetic nervous system (SNS) [3], which is a key regulator of metabolic rate. Thus, we considered that the SNS may play a role in mediating the metabolic adaption to ketosis.
Methods: To test this hypothesis, we measured the response of mice lacking all three β-adrenergic receptors (β-less mice) to KD feeding.
Results: In contrast to wild-type (WT) controls, β-less mice gained weight, increased adipose tissue depots mass, and did not increase energy expenditure when consuming KD. Remarkably, despite weight-gain, β-less mice were insulin sensitive. KD-induced changes in hepatic gene expression of β-less mice were similar to those seen in WT controls eating KD. Expression of FGF21 mRNA rose over 60-fold in both WT and β-less mice fed KD, and corresponding circulating FGF21 levels were 12.5 ng/ml in KD-fed wild type controls and 35.5 ng/ml in KD-fed β-less mice.
Conclusions: The response of β-less mice distinguishes at least two distinct categories of physiologic effects in mice consuming KD. In the liver, KD regulates peroxisome proliferator-activated receptor alpha (PPARα)-dependent pathways through an action of FGF21 independent of the SNS and beta-adrenergic receptors. In sharp contrast, induction of interscapular brown adipose tissue (BAT) and increased energy expenditure absolutely require SNS signals involving action on one or more β-adrenergic receptors. In this way, the key metabolic actions of FGF21 in response to KD have diverse effector mechanisms. |
topic |
Ketogenic diet Weight loss Sympathetic nervous system β-Adrenergic receptors |
url |
http://www.sciencedirect.com/science/article/pii/S2212877817303071 |
work_keys_str_mv |
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