Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I
Abstract The objective of the study was to report on a fatal case of feline toxoplasmosis with coinfection with the feline leukemia virus (FeLV). A domestic cat (Felis silvestris catus) presented intense dyspnea and died three days later. In the necropsy, the lungs were firm, without collapse and wi...
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Colégio Brasileiro de Parasitologia Veterinaria
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doaj-2ee76adf88e94728bc389648a59ce5bc2020-11-24T23:29:54ZengColégio Brasileiro de Parasitologia Veterinaria Revista Brasileira de Parasitologia Veterinária1984-296126217718410.1590/s1984-29612017025S1984-29612017000200177Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type IHilda Fátima de Jesus PenaCamila Mariellen EvangelistaRenata Assis CasagrandeGiovana BiezusClaudia Salete WisserPaulo Eduardo FerianAnderson Barbosa de MouraVeronica Machado RolimDavid DriemeierSolange OliveiraBruna Farias AlvesSolange Maria GennariSandra Davi TraversoAbstract The objective of the study was to report on a fatal case of feline toxoplasmosis with coinfection with the feline leukemia virus (FeLV). A domestic cat (Felis silvestris catus) presented intense dyspnea and died three days later. In the necropsy, the lungs were firm, without collapse and with many white areas; moderate lymphadenomegaly and splenomegaly were also observed. The histopathological examination showed severe necrotic interstitial bronchopneumonia and mild necrotic hepatitis, associated with intralesional cysts and tachyzoites of Toxoplasma gondii that were positive by anti-T. gondii immunohistochemical (IHC) evaluation. The bone marrow showed chronic myeloid leukemia and the neoplastic cells were positive by anti-FeLV IHC evaluation. DNA extracted from lungs was positive for T. gondii by PCR targeting REP-529. T. gondii was characterized by PCR-RFLP and by the microsatellites technique. ToxoDB-PCR-RFLP #10, i.e. the archetypal type I, was identified. Microsatellite analysis showed that the strain was a variant of type I with two atypical alleles. This was the first time that a T. gondii clonal type I genotype was correlated with a case of acute toxoplasmosis in a host in Brazil.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000200177&lng=en&tlng=enFelis silvestris catusFeLVgenotypingimmunohistochemistrymicrosatellite markers |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hilda Fátima de Jesus Pena Camila Mariellen Evangelista Renata Assis Casagrande Giovana Biezus Claudia Salete Wisser Paulo Eduardo Ferian Anderson Barbosa de Moura Veronica Machado Rolim David Driemeier Solange Oliveira Bruna Farias Alves Solange Maria Gennari Sandra Davi Traverso |
spellingShingle |
Hilda Fátima de Jesus Pena Camila Mariellen Evangelista Renata Assis Casagrande Giovana Biezus Claudia Salete Wisser Paulo Eduardo Ferian Anderson Barbosa de Moura Veronica Machado Rolim David Driemeier Solange Oliveira Bruna Farias Alves Solange Maria Gennari Sandra Davi Traverso Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I Revista Brasileira de Parasitologia Veterinária Felis silvestris catus FeLV genotyping immunohistochemistry microsatellite markers |
author_facet |
Hilda Fátima de Jesus Pena Camila Mariellen Evangelista Renata Assis Casagrande Giovana Biezus Claudia Salete Wisser Paulo Eduardo Ferian Anderson Barbosa de Moura Veronica Machado Rolim David Driemeier Solange Oliveira Bruna Farias Alves Solange Maria Gennari Sandra Davi Traverso |
author_sort |
Hilda Fátima de Jesus Pena |
title |
Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I |
title_short |
Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I |
title_full |
Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I |
title_fullStr |
Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I |
title_full_unstemmed |
Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I |
title_sort |
fatal toxoplasmosis in an immunosuppressed domestic cat from brazil caused by toxoplasma gondii clonal type i |
publisher |
Colégio Brasileiro de Parasitologia Veterinaria |
series |
Revista Brasileira de Parasitologia Veterinária |
issn |
1984-2961 |
description |
Abstract The objective of the study was to report on a fatal case of feline toxoplasmosis with coinfection with the feline leukemia virus (FeLV). A domestic cat (Felis silvestris catus) presented intense dyspnea and died three days later. In the necropsy, the lungs were firm, without collapse and with many white areas; moderate lymphadenomegaly and splenomegaly were also observed. The histopathological examination showed severe necrotic interstitial bronchopneumonia and mild necrotic hepatitis, associated with intralesional cysts and tachyzoites of Toxoplasma gondii that were positive by anti-T. gondii immunohistochemical (IHC) evaluation. The bone marrow showed chronic myeloid leukemia and the neoplastic cells were positive by anti-FeLV IHC evaluation. DNA extracted from lungs was positive for T. gondii by PCR targeting REP-529. T. gondii was characterized by PCR-RFLP and by the microsatellites technique. ToxoDB-PCR-RFLP #10, i.e. the archetypal type I, was identified. Microsatellite analysis showed that the strain was a variant of type I with two atypical alleles. This was the first time that a T. gondii clonal type I genotype was correlated with a case of acute toxoplasmosis in a host in Brazil. |
topic |
Felis silvestris catus FeLV genotyping immunohistochemistry microsatellite markers |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000200177&lng=en&tlng=en |
work_keys_str_mv |
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