Interleukin-6 is not essential for bone turnover in hypothyroid mice.
Interleukin-6 (IL-6) has been shown to be involved in the pathogenesis of several bone diseases characterized by an imbalance between bone resorption and formation. The aim of the study was to estimate serum markers of bone turnover: osteoclast-derived tartrate-resistant acid phosphatase form 5a (TR...
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Via Medica
2008-01-01
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Series: | Folia Histochemica et Cytobiologica |
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doaj-2eea495bd3e7472b8ba454b3e424fd682020-11-24T22:44:43ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312008-01-0145438739210.5603/4512Interleukin-6 is not essential for bone turnover in hypothyroid mice.Maria GorskaJerzy WaleckiZofia BondyraWlodzimierz MusialPiotr MysliwiecKarol KaminskiAgnieszkar NikolajukBoguslaw SawickiMaria WinnickaRobert ZbuckiJanusz MysliwiecInterleukin-6 (IL-6) has been shown to be involved in the pathogenesis of several bone diseases characterized by an imbalance between bone resorption and formation. The aim of the study was to estimate serum markers of bone turnover: osteoclast-derived tartrate-resistant acid phosphatase form 5a (TRACP 5b) and osteocalcin in IL-6-deficient mice to assess the role of IL-6 in bone metabolism in hypothyroidism in mice. C57BL/6J (wild-type; WT) and C57BL/6J(IL6-/-Kopf) (IL-6 knock-out; IL6KO) mice randomly divided into 4 groups with 10 in each one: 1/ WT mice in hypothyroidism (WT-ht), 2/ WT controls, 3/ IL6KO mice with hypothyroidism (IL6KO-ht) and 4/ IL6KO controls. Experimental model of hypothyroidism was induced by intraperitoneal injection of propylthiouracyl. The serum levels of TRACP 5b and osteocalcin were determined by ELISA. Serum concentrations of TRACP 5b (median and interquartile ranges) were significantly decreased in both groups of mice with hypothyroidism: WT (3.2 (2.5-4.7) U/l) and IL6KO (2.6 (1.8-3.5) U/l) as compared to the respective controls. Similarly, serum osteocalcin levels were significantly reduced in both groups of mice in experimental hypothyroidism: WT (25.8 (23.0-28.2) ng/ml) and IL6KO (21.5(19.0-24.6) ng/ml) in comparison to the respective controls. There were no significant differences in bone turnover markers between IL6KO and WT mice both in hypothyroid and control animals. The results of the present study suggest that IL-6 does not play an important role in bone turnover in both euthyroid and hypothyroid mice.http://czasopisma.viamedica.pl/fhc/article/view/4512 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria Gorska Jerzy Walecki Zofia Bondyra Wlodzimierz Musial Piotr Mysliwiec Karol Kaminski Agnieszkar Nikolajuk Boguslaw Sawicki Maria Winnicka Robert Zbucki Janusz Mysliwiec |
spellingShingle |
Maria Gorska Jerzy Walecki Zofia Bondyra Wlodzimierz Musial Piotr Mysliwiec Karol Kaminski Agnieszkar Nikolajuk Boguslaw Sawicki Maria Winnicka Robert Zbucki Janusz Mysliwiec Interleukin-6 is not essential for bone turnover in hypothyroid mice. Folia Histochemica et Cytobiologica |
author_facet |
Maria Gorska Jerzy Walecki Zofia Bondyra Wlodzimierz Musial Piotr Mysliwiec Karol Kaminski Agnieszkar Nikolajuk Boguslaw Sawicki Maria Winnicka Robert Zbucki Janusz Mysliwiec |
author_sort |
Maria Gorska |
title |
Interleukin-6 is not essential for bone turnover in hypothyroid mice. |
title_short |
Interleukin-6 is not essential for bone turnover in hypothyroid mice. |
title_full |
Interleukin-6 is not essential for bone turnover in hypothyroid mice. |
title_fullStr |
Interleukin-6 is not essential for bone turnover in hypothyroid mice. |
title_full_unstemmed |
Interleukin-6 is not essential for bone turnover in hypothyroid mice. |
title_sort |
interleukin-6 is not essential for bone turnover in hypothyroid mice. |
publisher |
Via Medica |
series |
Folia Histochemica et Cytobiologica |
issn |
0239-8508 1897-5631 |
publishDate |
2008-01-01 |
description |
Interleukin-6 (IL-6) has been shown to be involved in the pathogenesis of several bone diseases characterized by an imbalance between bone resorption and formation. The aim of the study was to estimate serum markers of bone turnover: osteoclast-derived tartrate-resistant acid phosphatase form 5a (TRACP 5b) and osteocalcin in IL-6-deficient mice to assess the role of IL-6 in bone metabolism in hypothyroidism in mice. C57BL/6J (wild-type; WT) and C57BL/6J(IL6-/-Kopf) (IL-6 knock-out; IL6KO) mice randomly divided into 4 groups with 10 in each one: 1/ WT mice in hypothyroidism (WT-ht), 2/ WT controls, 3/ IL6KO mice with hypothyroidism (IL6KO-ht) and 4/ IL6KO controls. Experimental model of hypothyroidism was induced by intraperitoneal injection of propylthiouracyl. The serum levels of TRACP 5b and osteocalcin were determined by ELISA. Serum concentrations of TRACP 5b (median and interquartile ranges) were significantly decreased in both groups of mice with hypothyroidism: WT (3.2 (2.5-4.7) U/l) and IL6KO (2.6 (1.8-3.5) U/l) as compared to the respective controls. Similarly, serum osteocalcin levels were significantly reduced in both groups of mice in experimental hypothyroidism: WT (25.8 (23.0-28.2) ng/ml) and IL6KO (21.5(19.0-24.6) ng/ml) in comparison to the respective controls. There were no significant differences in bone turnover markers between IL6KO and WT mice both in hypothyroid and control animals. The results of the present study suggest that IL-6 does not play an important role in bone turnover in both euthyroid and hypothyroid mice. |
url |
http://czasopisma.viamedica.pl/fhc/article/view/4512 |
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