Critical Minireview: The Fate of tRNACys during Oxidative Stress in Bacillus subtilis
Oxidative stress occurs when cells are exposed to elevated levels of reactive oxygen species that can damage biological molecules. One bacterial response to oxidative stress involves disulfide bond formation either between protein thiols or between protein thiols and low-molecular-weight (LMW) thiol...
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doaj-2f23236f1f2c419084be2d19c85b2a832020-11-24T21:40:51ZengMDPI AGBiomolecules2218-273X2017-01-0171610.3390/biom7010006biom7010006Critical Minireview: The Fate of tRNACys during Oxidative Stress in Bacillus subtilisJuan Campos Guillen0George H. Jones1Carlos Saldaña Gutiérrez2José Luis Hernández-Flores3Julio Alfonso Cruz Medina4José Humberto Valenzuela Soto5Sergio Pacheco Hernández6Sergio Romero Gómez7Verónica Morales Tlalpan8Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, 76010 Querétaro, Qro., MexicoDepartment of Biology, Emory University, Atlanta, GA 30322, USAFacultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, 76010 Querétaro, Qro., MexicoCentro de Investigación y de Estudios Avanzados del IPN, P.O. Box 629, 36500 Irapuato, GTO, MexicoFacultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, 76010 Querétaro, Qro., MexicoCONACYT, Centro de Investigación en Química Aplicada, Blvd. Enrique Reyna Hermosillo No. 140, 25294 Saltillo, Coahuila, MexicoFacultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, 76010 Querétaro, Qro., MexicoFacultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, 76010 Querétaro, Qro., MexicoFacultad de Medicina, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, 76010 Querétaro, Qro., MexicoOxidative stress occurs when cells are exposed to elevated levels of reactive oxygen species that can damage biological molecules. One bacterial response to oxidative stress involves disulfide bond formation either between protein thiols or between protein thiols and low-molecular-weight (LMW) thiols. Bacillithiol was recently identified as a major low-molecular-weight thiol in Bacillus subtilis and related Firmicutes. Four genes (bshA, bshB1, bshB2, and bshC) are involved in bacillithiol biosynthesis. The bshA and bshB1 genes are part of a seven-gene operon (ypjD), which includes the essential gene cca, encoding CCA-tRNA nucleotidyltransferase. The inclusion of cca in the operon containing bacillithiol biosynthetic genes suggests that the integrity of the 3′ terminus of tRNAs may also be important in oxidative stress. The addition of the 3′ terminal CCA sequence by CCA-tRNA nucleotidyltransferase to give rise to a mature tRNA and functional molecules ready for aminoacylation plays an essential role during translation and expression of the genetic code. Any defects in these processes, such as the accumulation of shorter and defective tRNAs under oxidative stress, might exert a deleterious effect on cells. This review summarizes the physiological link between tRNACys regulation and oxidative stress in Bacillus.http://www.mdpi.com/2218-273X/7/1/6bacillithiolBacillusoxidative stresstRNA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juan Campos Guillen George H. Jones Carlos Saldaña Gutiérrez José Luis Hernández-Flores Julio Alfonso Cruz Medina José Humberto Valenzuela Soto Sergio Pacheco Hernández Sergio Romero Gómez Verónica Morales Tlalpan |
spellingShingle |
Juan Campos Guillen George H. Jones Carlos Saldaña Gutiérrez José Luis Hernández-Flores Julio Alfonso Cruz Medina José Humberto Valenzuela Soto Sergio Pacheco Hernández Sergio Romero Gómez Verónica Morales Tlalpan Critical Minireview: The Fate of tRNACys during Oxidative Stress in Bacillus subtilis Biomolecules bacillithiol Bacillus oxidative stress tRNA |
author_facet |
Juan Campos Guillen George H. Jones Carlos Saldaña Gutiérrez José Luis Hernández-Flores Julio Alfonso Cruz Medina José Humberto Valenzuela Soto Sergio Pacheco Hernández Sergio Romero Gómez Verónica Morales Tlalpan |
author_sort |
Juan Campos Guillen |
title |
Critical Minireview: The Fate of tRNACys during Oxidative Stress in Bacillus subtilis |
title_short |
Critical Minireview: The Fate of tRNACys during Oxidative Stress in Bacillus subtilis |
title_full |
Critical Minireview: The Fate of tRNACys during Oxidative Stress in Bacillus subtilis |
title_fullStr |
Critical Minireview: The Fate of tRNACys during Oxidative Stress in Bacillus subtilis |
title_full_unstemmed |
Critical Minireview: The Fate of tRNACys during Oxidative Stress in Bacillus subtilis |
title_sort |
critical minireview: the fate of trnacys during oxidative stress in bacillus subtilis |
publisher |
MDPI AG |
series |
Biomolecules |
issn |
2218-273X |
publishDate |
2017-01-01 |
description |
Oxidative stress occurs when cells are exposed to elevated levels of reactive oxygen species that can damage biological molecules. One bacterial response to oxidative stress involves disulfide bond formation either between protein thiols or between protein thiols and low-molecular-weight (LMW) thiols. Bacillithiol was recently identified as a major low-molecular-weight thiol in Bacillus subtilis and related Firmicutes. Four genes (bshA, bshB1, bshB2, and bshC) are involved in bacillithiol biosynthesis. The bshA and bshB1 genes are part of a seven-gene operon (ypjD), which includes the essential gene cca, encoding CCA-tRNA nucleotidyltransferase. The inclusion of cca in the operon containing bacillithiol biosynthetic genes suggests that the integrity of the 3′ terminus of tRNAs may also be important in oxidative stress. The addition of the 3′ terminal CCA sequence by CCA-tRNA nucleotidyltransferase to give rise to a mature tRNA and functional molecules ready for aminoacylation plays an essential role during translation and expression of the genetic code. Any defects in these processes, such as the accumulation of shorter and defective tRNAs under oxidative stress, might exert a deleterious effect on cells. This review summarizes the physiological link between tRNACys regulation and oxidative stress in Bacillus. |
topic |
bacillithiol Bacillus oxidative stress tRNA |
url |
http://www.mdpi.com/2218-273X/7/1/6 |
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