4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation
Abstract As the most versatile and promising cell source, stem cells have been studied in regenerative medicine for two decades. Currently available culturing techniques utilize a 2D or 3D microenvironment for supporting the growth and proliferation of stem cells. However, these culture systems fail...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wiley
2020-03-01
|
Series: | Advanced Science |
Subjects: | |
Online Access: | https://doi.org/10.1002/advs.201902403 |
id |
doaj-2f5aba4e4b704b04a5ff632a3e2b70eb |
---|---|
record_format |
Article |
spelling |
doaj-2f5aba4e4b704b04a5ff632a3e2b70eb2020-11-25T02:38:06ZengWileyAdvanced Science2198-38442020-03-0176n/an/a10.1002/advs.2019024034D Self‐Morphing Culture Substrate for Modulating Cell DifferentiationShida Miao0Haitao Cui1Timothy Esworthy2Bhushan Mahadik3Se‐jun Lee4Xuan Zhou5Sung Yun Hann6John P. Fisher7Lijie Grace Zhang8Department of Mechanical and Aerospace Engineering The George Washington University 3590 Science and Engineering Hall, 800 22nd Street NW Washington DC 20052 USADepartment of Mechanical and Aerospace Engineering The George Washington University 3590 Science and Engineering Hall, 800 22nd Street NW Washington DC 20052 USADepartment of Mechanical and Aerospace Engineering The George Washington University 3590 Science and Engineering Hall, 800 22nd Street NW Washington DC 20052 USAFischell Department of Bioengineering University of Maryland 3238 Jeong H. Kim Engineering Building College Park MD 20742 USADepartment of Mechanical and Aerospace Engineering The George Washington University 3590 Science and Engineering Hall, 800 22nd Street NW Washington DC 20052 USADepartment of Mechanical and Aerospace Engineering The George Washington University 3590 Science and Engineering Hall, 800 22nd Street NW Washington DC 20052 USADepartment of Mechanical and Aerospace Engineering The George Washington University 3590 Science and Engineering Hall, 800 22nd Street NW Washington DC 20052 USAFischell Department of Bioengineering University of Maryland 3238 Jeong H. Kim Engineering Building College Park MD 20742 USADepartment of Mechanical and Aerospace Engineering The George Washington University 3590 Science and Engineering Hall, 800 22nd Street NW Washington DC 20052 USAAbstract As the most versatile and promising cell source, stem cells have been studied in regenerative medicine for two decades. Currently available culturing techniques utilize a 2D or 3D microenvironment for supporting the growth and proliferation of stem cells. However, these culture systems fail to fully reflect the supportive biological environment in which stem cells reside in vivo, which contain dynamic biophysical growth cues. Herein, a 4D programmable culture substrate with a self‐morphing capability is presented as a means to enhance dynamic cell growth and induce differentiation of stem cells. To function as a model system, a 4D neural culture substrate is fabricated using a combination of printing and imprinting techniques keyed to the different biological features of neural stem cells (NSCs) at different differentiation stages. Results show the 4D culture substrate demonstrates a time‐dependent self‐morphing process that plays an essential role in regulating NSC behaviors in a spatiotemporal manner and enhances neural differentiation of NSCs along with significant axonal alignment. This study of a customized, dynamic substrate revolutionizes current stem cell therapies, and can further have a far‐reaching impact on improving tissue regeneration and mimicking specific disease progression, as well as other impacts on materials and life science research.https://doi.org/10.1002/advs.2019024034D culture substratescell differentiationneural regenerationprogrammable culture substratesregenerative medicinestem cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shida Miao Haitao Cui Timothy Esworthy Bhushan Mahadik Se‐jun Lee Xuan Zhou Sung Yun Hann John P. Fisher Lijie Grace Zhang |
spellingShingle |
Shida Miao Haitao Cui Timothy Esworthy Bhushan Mahadik Se‐jun Lee Xuan Zhou Sung Yun Hann John P. Fisher Lijie Grace Zhang 4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation Advanced Science 4D culture substrates cell differentiation neural regeneration programmable culture substrates regenerative medicine stem cells |
author_facet |
Shida Miao Haitao Cui Timothy Esworthy Bhushan Mahadik Se‐jun Lee Xuan Zhou Sung Yun Hann John P. Fisher Lijie Grace Zhang |
author_sort |
Shida Miao |
title |
4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation |
title_short |
4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation |
title_full |
4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation |
title_fullStr |
4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation |
title_full_unstemmed |
4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation |
title_sort |
4d self‐morphing culture substrate for modulating cell differentiation |
publisher |
Wiley |
series |
Advanced Science |
issn |
2198-3844 |
publishDate |
2020-03-01 |
description |
Abstract As the most versatile and promising cell source, stem cells have been studied in regenerative medicine for two decades. Currently available culturing techniques utilize a 2D or 3D microenvironment for supporting the growth and proliferation of stem cells. However, these culture systems fail to fully reflect the supportive biological environment in which stem cells reside in vivo, which contain dynamic biophysical growth cues. Herein, a 4D programmable culture substrate with a self‐morphing capability is presented as a means to enhance dynamic cell growth and induce differentiation of stem cells. To function as a model system, a 4D neural culture substrate is fabricated using a combination of printing and imprinting techniques keyed to the different biological features of neural stem cells (NSCs) at different differentiation stages. Results show the 4D culture substrate demonstrates a time‐dependent self‐morphing process that plays an essential role in regulating NSC behaviors in a spatiotemporal manner and enhances neural differentiation of NSCs along with significant axonal alignment. This study of a customized, dynamic substrate revolutionizes current stem cell therapies, and can further have a far‐reaching impact on improving tissue regeneration and mimicking specific disease progression, as well as other impacts on materials and life science research. |
topic |
4D culture substrates cell differentiation neural regeneration programmable culture substrates regenerative medicine stem cells |
url |
https://doi.org/10.1002/advs.201902403 |
work_keys_str_mv |
AT shidamiao 4dselfmorphingculturesubstrateformodulatingcelldifferentiation AT haitaocui 4dselfmorphingculturesubstrateformodulatingcelldifferentiation AT timothyesworthy 4dselfmorphingculturesubstrateformodulatingcelldifferentiation AT bhushanmahadik 4dselfmorphingculturesubstrateformodulatingcelldifferentiation AT sejunlee 4dselfmorphingculturesubstrateformodulatingcelldifferentiation AT xuanzhou 4dselfmorphingculturesubstrateformodulatingcelldifferentiation AT sungyunhann 4dselfmorphingculturesubstrateformodulatingcelldifferentiation AT johnpfisher 4dselfmorphingculturesubstrateformodulatingcelldifferentiation AT lijiegracezhang 4dselfmorphingculturesubstrateformodulatingcelldifferentiation |
_version_ |
1724792858779582464 |