Production of Dextran from Sugar Cane Molasses by Leuconostoc mesenteroides

• Main Authors: M Faramarzi, Y Rahimi Kashkouli, HR RahimiKashkouli, D Gholamzadeh
• Format: Article
• Language: fas
• Published: Yasuj University Of Medical Sciences 2013-07-01
• Series: Armaghane Danesh Bimonthly Journal
• Subjects: Key words: Dextran; Molasses; Leuconostoc Mesenteroides
• Online Access: http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-225&slc_lang=en&sid=1

Abstract Background & aim: Dextran is a polysaccharide consisting of glucose monomers that are widely used in medicine as a blood volume extender. The aim of this study was to produce dextran from cane molasses using Leuconostoc mesenteroides bacteria. Methods: In this experimental study, for bacterial growth and dextran production, sugarcane molasses was added to the culture medium at different concentrations. Dextran sedimentation was obtained by shaking and centrifugation by adding ethanol after 48 hours. Response surface design was used for qualitative identification of the polarization of dextran and statistical analysis methods. Results: After assessing the separation and interactive effects of the parameters on the optimum amount of dextran produced from sugarcane molasses as 50 g, 35 º C and 5/8 = pH , the Dextran produced was more than 82 g/l. The correlation of the computational model for the dextran produced was 99.5%, which indicated excellent agreement with the experimental and computational models of high accuracy. Conclusion: Dextran produced by Leuconostoc mesenteroides bacteria and sugarcane molasses as substrate, is a cheap and affordable compared to current methods of dextran production. In addition to producing a clinical product, the molasses pollution could be dramatically decreased. Key words: Dextran, Molasses, Leuconostoc Mesenteroides