Emerging Technologies in Mass Spectrometry-Based DNA Adductomics
The measurement of DNA adducts, the covalent modifications of DNA upon the exposure to the environmental and dietary genotoxicants and endogenously produced electrophiles, provides molecular evidence for DNA damage. With the recent improvements in the sensitivity and scanning speed of mass spectrome...
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doaj-2f63706ae42e4d8d9794307f304fc2752020-11-25T01:31:22ZengMDPI AGHigh-Throughput2571-51352019-05-01821310.3390/ht8020013ht8020013Emerging Technologies in Mass Spectrometry-Based DNA AdductomicsJingshu Guo0Robert J. Turesky1Masonic Cancer Center and Department of Medicinal Chemistry, University of Minnesota, 2231 6th St. SE, Minneapolis, MI 55455, USAMasonic Cancer Center and Department of Medicinal Chemistry, University of Minnesota, 2231 6th St. SE, Minneapolis, MI 55455, USAThe measurement of DNA adducts, the covalent modifications of DNA upon the exposure to the environmental and dietary genotoxicants and endogenously produced electrophiles, provides molecular evidence for DNA damage. With the recent improvements in the sensitivity and scanning speed of mass spectrometry (MS) instrumentation, particularly high-resolution MS, it is now feasible to screen for the totality of DNA damage in the human genome through DNA adductomics approaches. Several MS platforms have been used in DNA adductomic analysis, each of which has its strengths and limitations. The loss of 2′-deoxyribose from the modified nucleoside upon collision-induced dissociation is the main transition feature utilized in the screening of DNA adducts. Several advanced data-dependent and data-independent scanning techniques originated from proteomics and metabolomics have been tailored for DNA adductomics. The field of DNA adductomics is an emerging technology in human exposure assessment. As the analytical technology matures and bioinformatics tools become available for analysis of the MS data, DNA adductomics can advance our understanding about the role of chemical exposures in DNA damage and disease risk.https://www.mdpi.com/2571-5135/8/2/13CarcinogensDNA adductsadductomicsmass spectrometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jingshu Guo Robert J. Turesky |
spellingShingle |
Jingshu Guo Robert J. Turesky Emerging Technologies in Mass Spectrometry-Based DNA Adductomics High-Throughput Carcinogens DNA adducts adductomics mass spectrometry |
author_facet |
Jingshu Guo Robert J. Turesky |
author_sort |
Jingshu Guo |
title |
Emerging Technologies in Mass Spectrometry-Based DNA Adductomics |
title_short |
Emerging Technologies in Mass Spectrometry-Based DNA Adductomics |
title_full |
Emerging Technologies in Mass Spectrometry-Based DNA Adductomics |
title_fullStr |
Emerging Technologies in Mass Spectrometry-Based DNA Adductomics |
title_full_unstemmed |
Emerging Technologies in Mass Spectrometry-Based DNA Adductomics |
title_sort |
emerging technologies in mass spectrometry-based dna adductomics |
publisher |
MDPI AG |
series |
High-Throughput |
issn |
2571-5135 |
publishDate |
2019-05-01 |
description |
The measurement of DNA adducts, the covalent modifications of DNA upon the exposure to the environmental and dietary genotoxicants and endogenously produced electrophiles, provides molecular evidence for DNA damage. With the recent improvements in the sensitivity and scanning speed of mass spectrometry (MS) instrumentation, particularly high-resolution MS, it is now feasible to screen for the totality of DNA damage in the human genome through DNA adductomics approaches. Several MS platforms have been used in DNA adductomic analysis, each of which has its strengths and limitations. The loss of 2′-deoxyribose from the modified nucleoside upon collision-induced dissociation is the main transition feature utilized in the screening of DNA adducts. Several advanced data-dependent and data-independent scanning techniques originated from proteomics and metabolomics have been tailored for DNA adductomics. The field of DNA adductomics is an emerging technology in human exposure assessment. As the analytical technology matures and bioinformatics tools become available for analysis of the MS data, DNA adductomics can advance our understanding about the role of chemical exposures in DNA damage and disease risk. |
topic |
Carcinogens DNA adducts adductomics mass spectrometry |
url |
https://www.mdpi.com/2571-5135/8/2/13 |
work_keys_str_mv |
AT jingshuguo emergingtechnologiesinmassspectrometrybaseddnaadductomics AT robertjturesky emergingtechnologiesinmassspectrometrybaseddnaadductomics |
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