Mathematical modelling of CRISPR-Cas system effects on biofilm formation
Clustered regularly interspaced short palindromic repeats (CRISPR), linked with CRISPR associated (Cas) genes, can confer adaptive immunity to bacteria, against bacteriophage infections. Thus from a therapeutic standpoint, CRISPR immunity increases biofilm resistance to phage therapy. Recently, howe...
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Online Access: | http://dx.doi.org/10.1080/17513758.2017.1314025 |
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doaj-2f69ac2747df4c34af6bf84ebdaf713b2020-11-25T01:14:55ZengTaylor & Francis GroupJournal of Biological Dynamics1751-37581751-37662017-08-0111026428410.1080/17513758.2017.13140251314025Mathematical modelling of CRISPR-Cas system effects on biofilm formationQasim Ali0Lindi M. Wahl1University of Western OntarioUniversity of Western OntarioClustered regularly interspaced short palindromic repeats (CRISPR), linked with CRISPR associated (Cas) genes, can confer adaptive immunity to bacteria, against bacteriophage infections. Thus from a therapeutic standpoint, CRISPR immunity increases biofilm resistance to phage therapy. Recently, however, CRISPR-Cas genes have been implicated in reducing biofilm formation in lysogenized cells. Thus CRISPR immunity can have complex effects on phage–host–lysogen interactions, particularly in a biofilm. In this contribution, we develop and analyse a series of dynamical systems to elucidate and disentangle these interactions. Two competition models are used to study the effects of lysogens (first model) and CRISPR-immune bacteria (second model) in the biofilm. In the third model, the effect of delivering lysogens to a CRISPR-immune biofilm is investigated. Using standard analyses of equilibria, stability and bifurcations, our models predict that lysogens may be able to displace CRISPR-immune bacteria in a biofilm, and thus suggest strategies to eliminate phage-resistant biofilms.http://dx.doi.org/10.1080/17513758.2017.1314025BacteriabacteriophagebiofilmCRISPR-Cas systemlysogens |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qasim Ali Lindi M. Wahl |
spellingShingle |
Qasim Ali Lindi M. Wahl Mathematical modelling of CRISPR-Cas system effects on biofilm formation Journal of Biological Dynamics Bacteria bacteriophage biofilm CRISPR-Cas system lysogens |
author_facet |
Qasim Ali Lindi M. Wahl |
author_sort |
Qasim Ali |
title |
Mathematical modelling of CRISPR-Cas system effects on biofilm formation |
title_short |
Mathematical modelling of CRISPR-Cas system effects on biofilm formation |
title_full |
Mathematical modelling of CRISPR-Cas system effects on biofilm formation |
title_fullStr |
Mathematical modelling of CRISPR-Cas system effects on biofilm formation |
title_full_unstemmed |
Mathematical modelling of CRISPR-Cas system effects on biofilm formation |
title_sort |
mathematical modelling of crispr-cas system effects on biofilm formation |
publisher |
Taylor & Francis Group |
series |
Journal of Biological Dynamics |
issn |
1751-3758 1751-3766 |
publishDate |
2017-08-01 |
description |
Clustered regularly interspaced short palindromic repeats (CRISPR), linked with CRISPR associated (Cas) genes, can confer adaptive immunity to bacteria, against bacteriophage infections. Thus from a therapeutic standpoint, CRISPR immunity increases biofilm resistance to phage therapy. Recently, however, CRISPR-Cas genes have been implicated in reducing biofilm formation in lysogenized cells. Thus CRISPR immunity can have complex effects on phage–host–lysogen interactions, particularly in a biofilm. In this contribution, we develop and analyse a series of dynamical systems to elucidate and disentangle these interactions. Two competition models are used to study the effects of lysogens (first model) and CRISPR-immune bacteria (second model) in the biofilm. In the third model, the effect of delivering lysogens to a CRISPR-immune biofilm is investigated. Using standard analyses of equilibria, stability and bifurcations, our models predict that lysogens may be able to displace CRISPR-immune bacteria in a biofilm, and thus suggest strategies to eliminate phage-resistant biofilms. |
topic |
Bacteria bacteriophage biofilm CRISPR-Cas system lysogens |
url |
http://dx.doi.org/10.1080/17513758.2017.1314025 |
work_keys_str_mv |
AT qasimali mathematicalmodellingofcrisprcassystemeffectsonbiofilmformation AT lindimwahl mathematicalmodellingofcrisprcassystemeffectsonbiofilmformation |
_version_ |
1725155581432430592 |