Tocotrienol-Rich Fraction from Palm Oil Prevents Oxidative Damage in Diabetic Rats
Objectives: This study was carried out to determine the effects of tocotrienol-rich fraction (TRF) (200 mg/Kg) on biomarkers of oxidative stress on erythrocyte membranes and leukocyte deoxyribonucleic acid (DNA) damage in streptozotocin (STZ)-induced diabetic rats. Methods: Male rats (n = 40) were d...
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doaj-2f73056acde14dd39c24797bf11a30392020-11-25T02:54:31ZengSultan Qaboos UniversitySultan Qaboos University Medical Journal 2075-051X2075-05282014-02-01141951031813Tocotrienol-Rich Fraction from Palm Oil Prevents Oxidative Damage in Diabetic RatsFatmah A. Matough0Siti B. Budin1Zariyantey A. Hamid2Mariati Abdul-Rahman3Nasar Al-Wahaibi4Jamaludine Mohammed5Department of Biology, Faculty of Science, Sabha University, Sabha, LibyaProgramme of Biomedical Sciences, School of Diagnostic Applied Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, MalayasiaProgramme of Biomedical Sciences, School of Diagnostic Applied Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, MalayasiaDepartment of Clinical Oral Biology, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur, MalayasiaDepartment of Pathology, College of Medicine & Health Sciences, Sultan Qaboos University, Muscat, OmanProgramme of Biomedical Sciences, School of Diagnostic Applied Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, MalayasiaObjectives: This study was carried out to determine the effects of tocotrienol-rich fraction (TRF) (200 mg/Kg) on biomarkers of oxidative stress on erythrocyte membranes and leukocyte deoxyribonucleic acid (DNA) damage in streptozotocin (STZ)-induced diabetic rats. Methods: Male rats (n = 40) were divided randomly into four groups of 10: a normal group; a normal group with TRF; a diabetic group, and a diabetic group with TRF. Following four weeks of treatment, fasting blood glucose (FBG) levels, oxidative stress markers and the antioxidant status of the erythrocytes were measured. Results: FBG levels for the STZ-induced diabetic rats were significantly increased (P <0.001) when compared to the normal group and erythrocyte malondialdehyde levels were also significantly higher (P <0.0001) in this group. Decreased levels of reduced glutathione and increased levels of oxidised glutathione (P <0.001) were observed in STZ-induced diabetic rats when compared to the control group and diabetic group with TRF. The results of the superoxide dismutase and glutathione peroxidase activities were significantly lower in the STZ-induced diabetic rats than in the normal group (P <0.001). The levels of DNA damage, measured by the tail length and tail moment of the leukocyte, were significantly higher in STZ-induced diabetic (P <0.0001). TRF supplementation managed to normalise the level of DNA damage in diabetic rats treated with TRF. Conclusion: Daily supplementation with 200 mg/Kg of TRF for four weeks was found to reduce levels of oxidative stress markers by inhibiting lipid peroxidation and increasing the levels of antioxidant status in a prevention trial for STZ-induced diabetic rats.https://journals.squ.edu.om/index.php/squmj/article/view/1889antioxidantsdiabetes mellitusoxidative stresstocotrienols. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fatmah A. Matough Siti B. Budin Zariyantey A. Hamid Mariati Abdul-Rahman Nasar Al-Wahaibi Jamaludine Mohammed |
spellingShingle |
Fatmah A. Matough Siti B. Budin Zariyantey A. Hamid Mariati Abdul-Rahman Nasar Al-Wahaibi Jamaludine Mohammed Tocotrienol-Rich Fraction from Palm Oil Prevents Oxidative Damage in Diabetic Rats Sultan Qaboos University Medical Journal antioxidants diabetes mellitus oxidative stress tocotrienols. |
author_facet |
Fatmah A. Matough Siti B. Budin Zariyantey A. Hamid Mariati Abdul-Rahman Nasar Al-Wahaibi Jamaludine Mohammed |
author_sort |
Fatmah A. Matough |
title |
Tocotrienol-Rich Fraction from Palm Oil Prevents Oxidative Damage in Diabetic Rats |
title_short |
Tocotrienol-Rich Fraction from Palm Oil Prevents Oxidative Damage in Diabetic Rats |
title_full |
Tocotrienol-Rich Fraction from Palm Oil Prevents Oxidative Damage in Diabetic Rats |
title_fullStr |
Tocotrienol-Rich Fraction from Palm Oil Prevents Oxidative Damage in Diabetic Rats |
title_full_unstemmed |
Tocotrienol-Rich Fraction from Palm Oil Prevents Oxidative Damage in Diabetic Rats |
title_sort |
tocotrienol-rich fraction from palm oil prevents oxidative damage in diabetic rats |
publisher |
Sultan Qaboos University |
series |
Sultan Qaboos University Medical Journal |
issn |
2075-051X 2075-0528 |
publishDate |
2014-02-01 |
description |
Objectives: This study was carried out to determine the effects of tocotrienol-rich fraction (TRF) (200 mg/Kg) on biomarkers of oxidative stress on erythrocyte membranes and leukocyte deoxyribonucleic acid (DNA) damage in streptozotocin (STZ)-induced diabetic rats. Methods: Male rats (n = 40) were divided randomly into four groups of 10: a normal group; a normal group with TRF; a diabetic group, and a diabetic group with TRF. Following four weeks of treatment, fasting blood glucose (FBG) levels, oxidative stress markers and the antioxidant status of the erythrocytes were measured. Results: FBG levels for the STZ-induced diabetic rats were significantly increased (P <0.001) when compared to the normal group and erythrocyte malondialdehyde levels were also significantly higher (P <0.0001) in this group. Decreased levels of reduced glutathione and increased levels of oxidised glutathione (P <0.001) were observed in STZ-induced diabetic rats when compared to the control group and diabetic group with TRF. The results of the superoxide dismutase and glutathione peroxidase activities were significantly lower in the STZ-induced diabetic rats than in the normal group (P <0.001). The levels of DNA damage, measured by the tail length and tail moment of the leukocyte, were significantly higher in STZ-induced diabetic (P <0.0001). TRF supplementation managed to normalise the level of DNA damage in diabetic rats treated with TRF. Conclusion: Daily supplementation with 200 mg/Kg of TRF for four weeks was found to reduce levels of oxidative stress markers by inhibiting lipid peroxidation and increasing the levels of antioxidant status in a prevention trial for STZ-induced diabetic rats. |
topic |
antioxidants diabetes mellitus oxidative stress tocotrienols. |
url |
https://journals.squ.edu.om/index.php/squmj/article/view/1889 |
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