Striatal Distribution and Cytoarchitecture of Dopamine Receptor Subtype 1 and 2: Evidence from Double-Labeling Transgenic Mice
As the main input nucleus of the basal ganglion, the striatum executes different functions, including motivation, reward and attention. The functions of the striatum highly rely on its subregions that receive projections from various cortical areas and the distribution of striatonigral neurons that...
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Frontiers Media S.A.
2017-08-01
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Series: | Frontiers in Neural Circuits |
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Online Access: | http://journal.frontiersin.org/article/10.3389/fncir.2017.00057/full |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Keke Ren Keke Ren Baolin Guo Chunqiu Dai Chunqiu Dai Han Yao Tangna Sun Xia Liu Zhantao Bai Wenting Wang Shengxi Wu |
spellingShingle |
Keke Ren Keke Ren Baolin Guo Chunqiu Dai Chunqiu Dai Han Yao Tangna Sun Xia Liu Zhantao Bai Wenting Wang Shengxi Wu Striatal Distribution and Cytoarchitecture of Dopamine Receptor Subtype 1 and 2: Evidence from Double-Labeling Transgenic Mice Frontiers in Neural Circuits striatum dopamine receptor subtype 1 dopamine receptor subtype 2 medium-sized spiny neurons BAC transgenic mice |
author_facet |
Keke Ren Keke Ren Baolin Guo Chunqiu Dai Chunqiu Dai Han Yao Tangna Sun Xia Liu Zhantao Bai Wenting Wang Shengxi Wu |
author_sort |
Keke Ren |
title |
Striatal Distribution and Cytoarchitecture of Dopamine Receptor Subtype 1 and 2: Evidence from Double-Labeling Transgenic Mice |
title_short |
Striatal Distribution and Cytoarchitecture of Dopamine Receptor Subtype 1 and 2: Evidence from Double-Labeling Transgenic Mice |
title_full |
Striatal Distribution and Cytoarchitecture of Dopamine Receptor Subtype 1 and 2: Evidence from Double-Labeling Transgenic Mice |
title_fullStr |
Striatal Distribution and Cytoarchitecture of Dopamine Receptor Subtype 1 and 2: Evidence from Double-Labeling Transgenic Mice |
title_full_unstemmed |
Striatal Distribution and Cytoarchitecture of Dopamine Receptor Subtype 1 and 2: Evidence from Double-Labeling Transgenic Mice |
title_sort |
striatal distribution and cytoarchitecture of dopamine receptor subtype 1 and 2: evidence from double-labeling transgenic mice |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neural Circuits |
issn |
1662-5110 |
publishDate |
2017-08-01 |
description |
As the main input nucleus of the basal ganglion, the striatum executes different functions, including motivation, reward and attention. The functions of the striatum highly rely on its subregions that receive projections from various cortical areas and the distribution of striatonigral neurons that express D1 dopamine (DA) receptors (or D1 medium-sized spiny neurons, D1 MSNs) and striatopallidal neurons that express D2 DA receptors (or D2 MSNs). Using bacterial artificial chromosome (BAC) transgenic mice, several studies have recently been performed on the spatial distribution of D1 and D2 MSNs. However, these studies mainly focused on enumeration of either D1-enhanced fluorescent protein (eGFP) or D2-eGFP in mice. In the present work, we used Drd1a-tdTamato and Drd2-eGFP double BAC transgenic mice to evaluate the spatial pattern of D1 MSNs (red fluorescence) and D2 MSNs (green fluorescence) along the rostro-caudal axis of the dorsal striatum. The dorsal striatum was divided into three subregions: rostral caudoputamen (CPr), intermediate CP (CPi), and caudal CP (CPc) across the rostral–caudal extent of the striatum. The results demonstrate that D1 and D2 MSNs were intermingled with each other in most of these regions. The cell density of D1 MSNs was slightly higher than D2 MSNs through CPr, CPi, and CPc, though it did not reach significance. However, in CPi, the ratio of D1/D2 in the ventromedial CPi group was significantly higher than those in dorsolateral, dorsomedial, and ventrolateral CPi. There was similar proportion of cells that co-expressed D1 and D2 receptors. Moreover, we demonstrated a pathway-specific activation pattern of D1 MSNs and D2 MSNs in a manic like mouse model induced by D-Amphetamine by utilizing this double transgenic mice and c-fos immunoreactivity. Our results may provide a morphological basis for the function or pathophysiology of striatonigral and striatopallidal neurons with diverse cortical inputs to the dorsal striatum. |
topic |
striatum dopamine receptor subtype 1 dopamine receptor subtype 2 medium-sized spiny neurons BAC transgenic mice |
url |
http://journal.frontiersin.org/article/10.3389/fncir.2017.00057/full |
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doaj-2f966857d9c74386a92d5488da61d34d2020-11-25T00:05:16ZengFrontiers Media S.A.Frontiers in Neural Circuits1662-51102017-08-011110.3389/fncir.2017.00057277445Striatal Distribution and Cytoarchitecture of Dopamine Receptor Subtype 1 and 2: Evidence from Double-Labeling Transgenic MiceKeke Ren0Keke Ren1Baolin Guo2Chunqiu Dai3Chunqiu Dai4Han Yao5Tangna Sun6Xia Liu7Zhantao Bai8Wenting Wang9Shengxi Wu10Department of Neurobiology and Collaborative Innovation Center for Brain Science, School of Basic Medicine, Fourth Military Medical UniversityXi’an, ChinaCollege of Life Sciences and Research Center for Resource Peptide Drugs, Shaanxi Engineering and Technological Research Center for Conversation and Utilization of Regional Biological Resources, Yanan UniversityYanan, ChinaDepartment of Neurobiology and Collaborative Innovation Center for Brain Science, School of Basic Medicine, Fourth Military Medical UniversityXi’an, ChinaDepartment of Neurobiology and Collaborative Innovation Center for Brain Science, School of Basic Medicine, Fourth Military Medical UniversityXi’an, ChinaThe Fifth Camp, The First Cadet Brigade, Fourth Military Medical UniversityXi’an, ChinaDepartment of Neurobiology and Collaborative Innovation Center for Brain Science, School of Basic Medicine, Fourth Military Medical UniversityXi’an, ChinaDepartment of Neurology, Tangdu Hospital, Fourth Military Medical UniversityXi’an, ChinaCollege of Life Sciences and Research Center for Resource Peptide Drugs, Shaanxi Engineering and Technological Research Center for Conversation and Utilization of Regional Biological Resources, Yanan UniversityYanan, ChinaCollege of Life Sciences and Research Center for Resource Peptide Drugs, Shaanxi Engineering and Technological Research Center for Conversation and Utilization of Regional Biological Resources, Yanan UniversityYanan, ChinaDepartment of Neurobiology and Collaborative Innovation Center for Brain Science, School of Basic Medicine, Fourth Military Medical UniversityXi’an, ChinaDepartment of Neurobiology and Collaborative Innovation Center for Brain Science, School of Basic Medicine, Fourth Military Medical UniversityXi’an, ChinaAs the main input nucleus of the basal ganglion, the striatum executes different functions, including motivation, reward and attention. The functions of the striatum highly rely on its subregions that receive projections from various cortical areas and the distribution of striatonigral neurons that express D1 dopamine (DA) receptors (or D1 medium-sized spiny neurons, D1 MSNs) and striatopallidal neurons that express D2 DA receptors (or D2 MSNs). Using bacterial artificial chromosome (BAC) transgenic mice, several studies have recently been performed on the spatial distribution of D1 and D2 MSNs. However, these studies mainly focused on enumeration of either D1-enhanced fluorescent protein (eGFP) or D2-eGFP in mice. In the present work, we used Drd1a-tdTamato and Drd2-eGFP double BAC transgenic mice to evaluate the spatial pattern of D1 MSNs (red fluorescence) and D2 MSNs (green fluorescence) along the rostro-caudal axis of the dorsal striatum. The dorsal striatum was divided into three subregions: rostral caudoputamen (CPr), intermediate CP (CPi), and caudal CP (CPc) across the rostral–caudal extent of the striatum. The results demonstrate that D1 and D2 MSNs were intermingled with each other in most of these regions. The cell density of D1 MSNs was slightly higher than D2 MSNs through CPr, CPi, and CPc, though it did not reach significance. However, in CPi, the ratio of D1/D2 in the ventromedial CPi group was significantly higher than those in dorsolateral, dorsomedial, and ventrolateral CPi. There was similar proportion of cells that co-expressed D1 and D2 receptors. Moreover, we demonstrated a pathway-specific activation pattern of D1 MSNs and D2 MSNs in a manic like mouse model induced by D-Amphetamine by utilizing this double transgenic mice and c-fos immunoreactivity. Our results may provide a morphological basis for the function or pathophysiology of striatonigral and striatopallidal neurons with diverse cortical inputs to the dorsal striatum.http://journal.frontiersin.org/article/10.3389/fncir.2017.00057/fullstriatumdopamine receptor subtype 1dopamine receptor subtype 2medium-sized spiny neuronsBAC transgenic mice |